LOCUS       sequence-206172-       12720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express Cpf1 from EFS promoter, as well as a 
            Cpf1 FlipArray from U6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12720)
  AUTHORS   Chow RD, Kim HR, Chen S
  TITLE     Programmable sequential mutagenesis by inducible Cpf1 crRNA array 
            inversion.
  JOURNAL   Nat Commun. 2018 May 15;9(1):1903. doi: 10.1038/s41467-018-04158-z.
  PUBMED    29765043
REFERENCE   2  (bases 1 to 12720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-15"; volume: "9(1):1903. doi"; pages: " 
            10.1038/s41467-018-04158-z"
COMMENT     Sequence Label: pLenti-LbCpf1-U6_FlipArray
FEATURES             Location/Qualifiers
     source          1..12720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(3635..3655)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3658..3667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3675..3886)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     promoter        3937..4185
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3937..3957
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4108..4127
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(4214..4247)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4283..4316
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4371..4488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4786..4830)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5015..5248
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5741..5866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5913..6093
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6111..6309)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6136..6156)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6311..6690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6862..6881
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6956..7060
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7061..7129,7130..7921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7279..7298)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8092..8680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8581..8600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8834..8851
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8968..8989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9004..9009,9010..9027,9028..9034)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9042..9058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9047..9069
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9066..9082
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9066..9082
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9131..9252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9142..9161)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9196..9215
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9382..9756)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(9775..9822)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(9870..10199)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9884..10019
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9938..9957)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     10184..10204
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(10213..10641)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10323..10344)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10535..10554
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    10687..10911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     10900..10917
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             10943..11176
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    11248..11836
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             11354..11365
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     11763..11783
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11852..12448)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11935..11955)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(12449..12505)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(12515..12595)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(12602..12649)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(join(12650..12720,1..3610))
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
ORIGIN
        1 tctcgtcctc ggccttcttg aactggccga tggcccacag cacctttctg gcgatgttat
       61 aggcgccatt ggcgtcggcg ttctttggca ggatggcatt ctcctgggcc tcatagttcc
      121 ggctatcgta gaagatgccg tcggagttct tcacagggct gatcagaaaa tccacgtcgg
      181 tgcggcctgt gatgctgttc cgcatctgca gcatcaggct catcagggcc ataaagctag
      241 agtagaaggc cttgtcggac tgctcgcaca gcagggctct gatatcgccc tgctgataat
      301 tgatgccgta cttgttgaac agctccttat aggcgctggt caggcacacc tcctcccagt
      361 cgaacacgtt gttcttctta ggattccgga agattctgat ccggttgccg taggagtaca
      421 gcttccactt cttgatgtaa tcggcgtctg tgcgagagaa gttcttatag tccagggcaa
      481 actcgaacag atcctcctcg ggcacgtaca tgatcctgtc aaaggagctg atgaacttct
      541 tggaatcggc gatgctggta tacttggttt tcagcaggtt cacaaagccg gtagatggat
      601 cgatcttgga tgtcagccag gcagggatgt aaaagatgaa gccgttctgg gtagacatgg
      661 acttaaagct ctcgaactta ttggtgatct gatagccctt cagggcgccg cctgttgcac
      721 aaggattaga cttcttgtcc accatgtagt tcagcttatc gatcagcatc ttctcgaact
      781 tctgatacac ctgcttctcc accttcacgc ggctattctt aaagccagag ttcaggtcct
      841 ccagggcgat cacggcatcg tacttctcca ccagctcgca gatcttgtgc accacctgag
      901 agatatagcc ggccttcagc tccttgatat tctcgatgga ggtccagttc tggcgggcct
      961 cgaacctctc cttctccttc ttgtccagca gagagtggta atctgtcttg atcctgatgc
     1021 cgttgaagtt gttgatgatc tcgttcaggg aatactgctc cacgatgttg cccttgccgt
     1081 ccaccaccac gatatacagc agattgcgct cgcccctatc gatgccgatc acataggggt
     1141 tatcgtcgtg cttcagcagc acgcgcacct ctgtattgat cttgaagatg ttcttggggc
     1201 acttattgat ggcgattggg atgtgcagct cgtactggtc ctcagaaaac ctcttatcct
     1261 tatacacgtc gtaggacagg gttgtggttt tcttgggatt atctggattc ttgttggcga
     1321 taggggagtt ggctgggtgc accaccagct cctccttctt cagggaggcg cgcctcatga
     1381 acagctctgc tcctccgctc agcctgatct gtccgtgatt gttctcgtca aacagcagct
     1441 tgaagtacat ggtgtgcaga ttgggtgtgc cgtgagactt atcggaaaag tccttgttat
     1501 agatctggaa catatacagc ttgccctcct ccaccagctt atccacctcc ttcttgctgg
     1561 cagactcgaa gctcacctta tagccctgct cctccacctc tctgtaaaag ccggcgatgt
     1621 ccttatactt ctctgtctca gaaaagttga aatcgtaggc attggaccac tttggatacc
     1681 gggagatgct atccttaaag aagtcgatca gcttgtgaca gtcattcagg ttaaacatat
     1741 cgcccttctt gaatgtgcca ttcttgtaga tcttctggat gtcctcgctg gggttatagt
     1801 aggccatcca cttcttagaa aagaacacct ttggcagcat cttattaggg ccgggcagca
     1861 gcttatagtt gatcttctcg taattgccgt tcacatcgtc cttgtcgatc ttctgcaggc
     1921 acttggcgta cttcttatcc atgatggcca gatagtactt ggagccgtat ctcaggatgg
     1981 tggcccgata gtctgtctcc ttatccttgt cccagccgcc catgaactga gggttctgaa
     2041 aatacagctt gaacttatcc ttagagtagg gcttctgggt cacataattg cggatggcat
     2101 cgtagatgtg gtccaccttc agcaggatgt cgtaggccag cacaaaatcg ccatagaagg
     2161 actcgtccct gtttgtctcc ttgccctcgc caaagaaggc cttgatgtaa ttctcgaagc
     2221 tcttcacaga atccagcagg tccttcatga tggccaccac ggcgtcgttc ttcttcaggc
     2281 tcttctccag cacaaaatcg gcgtcgaaca gcttctcaga ggagccatac accttgtaga
     2341 tctcatccac cttctggatg atgatctcct tcagcttctc caccacagac agatcggcgt
     2401 cggcgtactc ctgcagctgc tccagagaaa aggagccgat cttcttgaag gactttctcc
     2461 gatcgtcctc gtacttctcg gtcaccacgg ccttcttctt caggtggata tcgtcatact
     2521 cggcattcca cttgtcccgg atcacgttcc actcgccgaa gatatccttg gagattgtgc
     2581 tgatggcggg gccgttcttc acaaagatgc cggcgctaga gtactcgtca aaattcttga
     2641 acagcttctc cagcttcttg atggagctga agatctcgct gttcttgttc agggtgtttc
     2701 taaacacctc cagcacctcc tcatcggatg tatagccctc gccgtagaag ctcagagact
     2761 cccgatcgct cagcacctgc ttatacagtg gcttaaactt aggcagcttc tgcttggttt
     2821 tctgattata caggttgatg tactcgttca ggcccttgat cttctcgccg ctctcggtca
     2881 cgaagccgcc gatgatggcg ttatacacgt cgatgccctc ctgtgtcagc acaaagttaa
     2941 agaactcgcc ctcaaagaaa tcctccacat catagtcgct gttcaggatc ttctccttga
     3001 tctcctgcac ctcgtgctta tcaaagatgg cgtccacctt ctcgaagatg tccatattag
     3061 agatgtagcg ggtcagattc tcgttgatac acctgaaggc gatggatgtg ctcttggcct
     3121 cctcggaaaa catattctct ctgttatcaa agaagccggt gaaggctgtg gtaaagccat
     3181 tgaagctgtt caccagggcg atctcgtcct tatcgtccag gaactctggc aggattgtct
     3241 cgatgatatc cttcttaaac agggacttgt agccctcgtt gcccttgaag gccttggcga
     3301 tctccttccg cagattgatc tccaggttct ccagctcctt attctccttc tcggttctgg
     3361 ttttcttccg gaacaggctg atgtaattgt tcagattctt cagcttgatg ctgtgcagca
     3421 cgtcgttgat aaaagacaga tagtagcgat ccagcagctt cttcacgccc ttataatcct
     3481 cggctctctt ctcgtcctcc accagcagcc gcttattgtc gatgttctcc tgggtcttgc
     3541 ccacagggat ggccttgaac ctcagggtct tagacaggga gtagcagttt gtaaacttct
     3601 ccagcttgct ggctgctggg actccgtgga taccgacctt ccgcttcttc tttggggcca
     3661 tggtggcacc ggacctgtgt tctggcggca aacccgttgc gaaaaagaac gttcacggcg
     3721 actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag ccagtacacg
     3781 acatcacttt cccagtttac cccgcgccac cttctctagg caccggatca attgccgacc
     3841 cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg acgggcaccg
     3901 gagccaattc ccactccttt caagacctct cgagaagagg gcctatttcc catgattcct
     3961 tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta
     4021 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     4081 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     4141 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgtaat ttctactaag
     4201 tgtagatagc gctataactt cgtatagcat acattatacg aacggtataa tttctactaa
     4261 gtgtagatga gacggacgtc tcataacttc gtataatgta tgctatacga acggtatttt
     4321 tttaattaaa ttaaccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga
     4381 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
     4441 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat
     4501 actgccattt gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta atcgaatgga
     4561 tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct
     4621 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
     4681 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
     4741 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
     4801 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
     4861 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
     4921 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
     4981 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
     5041 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
     5101 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
     5161 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
     5221 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
     5281 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
     5341 atataattca cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg
     5401 ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt
     5461 ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct actgtattat
     5521 ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt
     5581 gtctacagcc ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc
     5641 tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa
     5701 ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc
     5761 tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc
     5821 ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc
     5881 ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa
     5941 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     6001 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     6061 ccaggctcag atctggtcta accagagaga cccagtacag gcaaaacgcg ctgcttatat
     6121 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
     6181 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
     6241 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
     6301 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     6361 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     6421 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     6481 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     6541 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     6601 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     6661 actattaata actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa
     6721 gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc
     6781 ttgttgtagc ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga
     6841 gcagatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccataggg
     6901 gatcgggaga tctcccgatc cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg
     6961 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7021 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7081 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7141 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7201 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7261 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7321 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7381 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7441 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7501 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7561 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7621 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7681 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7741 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7801 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7861 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7921 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7981 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8221 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8761 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8821 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8881 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8941 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     9001 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9061 tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg tcgacggtat
     9121 acagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     9181 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     9241 aataaacaag ttggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
     9301 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
     9361 atcgaaatct cgtagcacgt gtcagtcctg ctcctcggcc acgaagtgca cgcagttgcc
     9421 ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct cggtcatggc
     9481 cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg cgtacagctc
     9541 gtccaggccg cgcacccaca cccaggccag ggtgttgtcc ggcaccacct ggtcctggac
     9601 cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa
     9661 gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc
     9721 ggtgagcacc ggaacggcac tggtcaactt ggccatggtt tagttcctca ccttgtcgta
     9781 ttatactatg ccgatatact atgccgatga ttaattgtca acacgtgctg atcagatccg
     9841 aaaatggata tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct
     9901 cctcactact tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa
     9961 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
    10021 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
    10081 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
    10141 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
    10201 ttccacagaa ttaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
    10261 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
    10321 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
    10381 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
    10441 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
    10501 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
    10561 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
    10621 atgcgccgct acagggcgcg tggggatacc ccctagagcc ccagctggtt ctttccgcct
    10681 cagaagccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc
    10741 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg
    10801 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg
    10861 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg
    10921 atcagcgggt ttaaacgggc cctgctagag attttccaca ctgactaaaa gggtctgagg
    10981 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
    11041 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
    11101 atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct tcgttgggag
    11161 tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc
    11221 ttgtaagtca ttggtcttaa agtcgacgcg gggaggcggc ccaaagggag atccgactcg
    11281 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
    11341 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
    11401 aggtggcaac acaggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg
    11461 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
    11521 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
    11581 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
    11641 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
    11701 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
    11761 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
    11821 aatccagagg ttgattgtcg acttaacgcg ttcaggcacc gggcttgcgg gtcatgcacc
    11881 aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt
    11941 agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg
    12001 ccgcctccac tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg
    12061 agactccgac ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc
    12121 cttccatctg ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga
    12181 tctcggcgaa caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg
    12241 cgccgtcgtc cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt
    12301 cttgcagctc ggtgacccgc tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg
    12361 ggtagtcggc gaacgcggcg gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg
    12421 cgaggcgcac cgtgggcttg tactcggtcg gtccaggatt ctcttcgaca tctccggctt
    12481 gtttcagcag agagaagttt gttgcgccgg atccggcata gtcggggaca tcatatgggt
    12541 atgcataatc aggcacgtcg tagggataag cgtaatctgg aacatcgtat gggtaggatc
    12601 cctttttctt ttttgcctgg ccggcctttt tcgtggccgc cggccttttg tgcttcacgc
    12661 tggtctgggc gtactccagc cactccttgt tagagatggc gatcttcacc ttatccagct
//
