LOCUS       sequence-206181-       12542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EasyCloneYALI system-based yeast integrative vector expressing Cas9 
            carrying hygromycin resistance marker, integration into Yarrowia 
            lipolytica chromosomal location IntB, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Holkenbrink C, Dam MI, Kildegaard KR, Beder J, Dahlin J, Domenech 
            Belda D, Borodina I
  TITLE     EasyCloneYALI: CRISPR/Cas9-Based Synthetic Toolbox for Engineering 
            of the Yeast Yarrowia lipolytica.
  JOURNAL   Biotechnol J. 2018 Jan 29. doi: 10.1002/biot.201700543.
  PUBMED    29377615
REFERENCE   2  (bases 1 to 12542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2018 Jan 29. doi: 10.1002/biot.201700543."
COMMENT     Sequence Label: pCfB4906
FEATURES             Location/Qualifiers
     source          1..12542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1311..5414
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1311..5414
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5427..5447
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    6820..6853
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7856..8884
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFNVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     complement(8901..8919)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    9118..9151
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(9737..9770)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(9977..9996)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(10002..10021)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        10063..10154
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10155..10223,10224..11015)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10373..10392)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11137..11725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11626..11645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11879..11896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     11994..12011
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11995..12011
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12481..12514
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gaatgcgtgc gatcccacag ttctcactca gatcatggag actctaacct tgagacatca
       61 attatcagct ctcgaggata atgttagtgc agttccagga ctcattgtgc aactgtcacc
      121 acggcatttt gggtctgttc ttttgaagta cagaaatatc ctcattgttg gtatactttg
      181 ggactttttc ttgttacaga agaataaaaa aacctcgact gatgtactaa ttacatggtt
      241 aacatcccca aggtcaaagt acagatattg taccgacttc tgaaatttgt gggatccaca
      301 cacggacctc tgcgatgata caatatcatg gttccatggt ctcttgaatc acaccactca
      361 ataataaaca ccagctcttt caatccaact tagctttctt ccacttgaac ataggccatc
      421 cccccttctt tctattttac ataaatagca agatccttac ccctacatgt ctcataacac
      481 aatctcaact tgacttccca taagaagttc actcagtcat gaaagtctta gtactggacg
      541 tgcaacgctt cagatgtgac catatactta ggcacgcaac taacatgaat gaatacgata
      601 tacatcaaag actatgatac gcagtattgc acactgtacg agtaagggca ctagccactg
      661 cactcaagtg aaaccgttgc ccgggtacga gtatgagtat gtacagtatg tttagtattg
      721 tacttggaca atgcttgtat cgtacattct caagtgtcaa acataaatat ccgttgctat
      781 atcctcgcac caccacgtag ctcgctatat ccctgtgttg aatccatcca tcttggattg
      841 ccaattgtgc acacagaacc gggcactcac ttccccatcc acacttcaac ggaatgcgtg
      901 cgatagagac cgggttggcg gcgcatttgt gtcccaaaaa acagccccaa ttgccccaat
      961 tgaccccaaa ttgacccagt agcgggccca accccggcga gagccccctt ctccccacat
     1021 atcaaacctc ccccggttcc cacacttgcc gttaagggcg tagggtactg cagtctggaa
     1081 tctacgcttg ttcagacttt gtactagttt ctttgtctgg ccatccgggt aacccatgcc
     1141 ggacgcaaaa tagactactg aaaatttttt tgctttgtgg ttgggacttt agccaagggt
     1201 ataaaagacc accgtccccg aattaccttt cctcttcttt tctctctctc cttgtcaact
     1261 cacacccgaa atcgttaagc atttccttct gagtataaga atcattcaaa atggacaaga
     1321 agtactctat cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg
     1381 agtacaaggt gccctctaag aagttcaagg tcctgggcaa caccgaccga cactctatca
     1441 agaagaacct gatcggcgcc ctgctgttcg actctggcga gactgccgag gccacccgac
     1501 tgaagcgaac cgcccgacga cgatacaccc gacgaaagaa ccgaatctgc tacctgcaag
     1561 agatcttctc taacgagatg gccaaggtgg acgactcgtt cttccaccga ctcgaggaat
     1621 ctttcctggt cgaggaagat aagaagcacg agcgacaccc catcttcggc aacatcgtgg
     1681 acgaggtggc ctaccacgag aagtacccca ccatctacca cctccgaaag aagctggttg
     1741 actctaccga caaggccgac ctgcgactga tctacctggc cctggcccac atgatcaagt
     1801 tccgaggcca cttcctgatc gagggcgacc tgaaccccga caactctgac gtggacaagc
     1861 tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcct
     1921 ctggcgtgga cgccaaggcc atcctgtctg cccgactgtc taagtctcga cgactcgaga
     1981 acctgattgc ccagctgccc ggcgagaaga agaacggcct gttcggcaac ctgatcgccc
     2041 tgtctctggg cctgaccccc aacttcaagt ctaacttcga cctggccgag gacgccaagc
     2101 tgcagctgtc taaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg
     2161 accagtacgc cgacctgttc ctggccgcca agaacctgtc tgacgccatc ctgctgtctg
     2221 acatcctgcg agtgaacacc gagatcacca aggcccccct gtccgcctct atgatcaagc
     2281 gatacgacga gcaccaccag gacctgaccc tgctgaaggc cctggtgcga cagcagctgc
     2341 ccgagaagta caaagagatt ttcttcgacc agtctaagaa cggctacgcc ggctacatcg
     2401 acggcggagc ctctcaagag gaattctaca agttcatcaa gcccatcctc gagaagatgg
     2461 acggcaccga ggaactgctg gtgaagctga accgagagga cctgctgcga aagcagcgaa
     2521 ccttcgacaa cggctctatc ccccaccaga tccacctggg cgagctgcac gccattctgc
     2581 gacgacaaga ggacttctac cccttcctga aagacaaccg agagaagatc gagaagattc
     2641 tgaccttccg aatcccctac tacgtgggcc ccctggcccg aggcaactct cgattcgcct
     2701 ggatgacccg aaagtctgag gaaactatca ccccctggaa cttcgaggaa gtggtggaca
     2761 agggcgcctc tgcccagtct ttcatcgagc gaatgaccaa cttcgacaag aacctgccca
     2821 acgagaaggt gctgcccaag cactctctgc tgtacgagta cttcaccgtg tacaacgagc
     2881 tgaccaaggt gaagtacgtg accgagggaa tgcgaaagcc cgccttcctg tctggcgagc
     2941 agaagaaggc catcgttgac ctgctgttca agaccaaccg aaaggtgacc gtgaagcagc
     3001 tgaaagagga ctacttcaaa aagatcgagt gcttcgactc cgtcgagatc tctggcgtcg
     3061 aggaccgatt caacgcctct ctgggcacct accacgacct gctgaagatt atcaaggaca
     3121 aggacttcct ggacaacgag gaaaacgagg acatcctcga ggacatcgtg ctgaccctga
     3181 ccctgttcga ggaccgagag atgattgagg aacgactcaa gacctacgcc cacctgttcg
     3241 acgacaaggt gatgaagcag ctcaagcgac gacgatacac tggctggggc cgactgtctc
     3301 gaaagctgat caacggcatc cgagacaagc agtctggcaa gaccatcctg gactttctga
     3361 agtctgacgg cttcgccaac cgaaacttca tgcagctgat ccacgacgac tccctgacct
     3421 tcaaagagga catccagaag gcccaggttt ctggccaggg cgactctctg cacgagcaca
     3481 ttgccaacct ggccggctct cccgccatca agaagggcat cctgcagacc gtgaaggtgg
     3541 tggacgagct ggtgaaggtg atgggccgac acaagcccga gaacatcgtg atcgagatgg
     3601 cccgagagaa ccagaccacc cagaagggcc agaagaactc tcgagagcga atgaagcgaa
     3661 tcgaggaagg catcaaagaa ctgggctctc agatcctgaa agaacacccc gtcgagaaca
     3721 cccagctgca gaacgagaag ctgtacctgt actacctgca gaacggccga gacatgtacg
     3781 tggaccaaga actggacatc aaccgactgt ctgactacga cgtggaccat atcgtgcccc
     3841 agtcttttct gaaggacgac tctatcgaca acaaggtgct gacccgatct gacaagaacc
     3901 gaggcaagtc tgacaacgtg ccctctgaag aagtggtcaa gaagatgaag aactactggc
     3961 gacagctgct gaacgccaag ctgatcaccc agcgaaagtt cgacaacctg accaaggccg
     4021 agcgaggcgg cctgtctgag ctggacaagg ccggcttcat caagcgacag ctggtcgaga
     4081 ctcgacagat caccaagcac gtggcccaga tcctggactc tcgaatgaac accaagtacg
     4141 acgagaacga caagctgatt cgagaggtga aggtcatcac cctgaagtct aagctggtgt
     4201 ctgacttccg aaaggacttc cagttctaca aggtgcgaga gatcaacaac taccaccacg
     4261 cccacgacgc ctacctgaac gccgtggtgg gcaccgccct gatcaagaag taccctaagc
     4321 tcgagtctga gttcgtgtac ggcgactaca aggtgtacga cgtgcgaaag atgatcgcca
     4381 agtctgagca agagatcggc aaggctaccg ccaagtactt cttctactct aacatcatga
     4441 actttttcaa gaccgagatt accctggcca acggcgagat ccgaaagcga cccctgatcg
     4501 agactaacgg cgagactggc gagatcgtgt gggacaaggg ccgagacttc gccaccgtgc
     4561 gaaaggtgct gtctatgccc caggtgaaca tcgtgaagaa gaccgaggtc cagaccggcg
     4621 gcttctctaa agagtctatc ctgcccaagc gaaactctga caagctgatc gcccgaaaga
     4681 aggactggga ccccaagaag tacggcggct tcgactctcc caccgtggcc tactctgtgc
     4741 tggtggtggc caaggtcgag aagggcaagt ctaagaagct gaagtctgtc aaagagctgc
     4801 tcggcatcac tatcatggaa cgatcttcgt tcgagaagaa ccctatcgac ttcctcgagg
     4861 ccaagggcta caaagaggtg aagaaggacc tgatcatcaa gctgcccaag tactccctgt
     4921 tcgagctgga gaacggccga aagcgaatgc tggcctctgc cggcgagctg cagaagggca
     4981 acgagctggc cctgccctct aagtacgtga acttcctgta cctggcctct cactacgaga
     5041 agctgaaggg ctctcccgag gacaacgagc agaagcagct gttcgtcgag cagcacaagc
     5101 actacctgga cgagatcatc gagcagatct ctgagttctc taagcgagtg atcctggccg
     5161 acgccaacct ggacaaggtg ctgtccgcct acaacaagca ccgagacaag cccatccgag
     5221 agcaggccga gaacatcatc cacctgttca ccctgaccaa cctgggagcc cccgctgcct
     5281 tcaagtactt cgacaccacc atcgaccgaa agcgatacac ctctaccaaa gaggtgctgg
     5341 acgccaccct gatccaccag tctatcaccg gcctgtacga gactcgaatc gacctgtctc
     5401 agctgggcgg cgactctcga gccgacccta agaagaagcg aaaggtctaa ccccacgttg
     5461 ccggtcttgc ctcctactac ctgtccatca atgacgaggt tctcacccct gcccaggtcg
     5521 aggctcttat tactgagtcc aacaccggtg ttcttcccac caccaacctc aagggctctc
     5581 ccaacgctgt tgcctacaac ggtgttggca tttaggcaat taacagatag tttgccggtg
     5641 ataattctct taacctccca cactcctttg acataacgat ttatgtaacg aaactgaaat
     5701 ttgaccagat attgttgtaa atagaaaatc tggcttgtag gtggcaaaat cccgtctttg
     5761 ttcatcaatt ccctctgtga ctactcgtca tccctttatg ttcgactgtc gtatttttat
     5821 tttccataca tacgcaagtg agatgcccgt gtccgaattc atcgcgtgca ttccttctgt
     5881 tcggaatcaa cctcaaggtt aacggccacg atcccctcgt tgttactctt ggtcagccca
     5941 ttgtcggtaa cgctggcttt gctaactggg tcgataaact cttctttggc caggagaacc
     6001 ccgatgtctc caaggtgtcc aaagaccgaa agctctaccg aatcacccac cgaggagata
     6061 tcgtccctca agtgcccttc tgggacggtt accagcactg ctctggtgag gtctttattg
     6121 actggcccct gatccaccct cctctctcca acgttgtcat gtgccagggc cagagcaata
     6181 aacagtgctc tgccggtaac actctgctcc agcaggtcaa tgtgattgga aaccatctgc
     6241 agtacttcgt caccgagggt gtctgtggta tctaagctat ttatcactct ttacaacttc
     6301 tacctcaact atctacttta ataaatgaat atcgtttatt ctctatgatt actgtatatg
     6361 cgttcctcta agacaaatcg aaaccagcat gcgatcgaat ggcatacaaa agtttcttcc
     6421 gaagttgatc aatgtcctga tagtcaggca gcttgagaag attgacacag gtggaggccg
     6481 tagggaaccg atcaacctgt ctaccagcgt tacgaatggc aaatgacggg ttcaaagcct
     6541 tgaatccttg caatggtgcc ttggatactg atgtcacaaa cttaagaagc agccgcttgt
     6601 cctcttcctc gaaactctca aacacagtcc agaagtcctt tatagtttga tctgtatcca
     6661 gatagcctcc gtaattggtg tgtgtcttca aatcccagac gtccacattg gcatgtcctc
     6721 cactgataag catttgaagt tcatctgcgt tgaacattga gacccacgaa gggtcaatga
     6781 gctggtatag accgcccaag aatgcatctg atctgtcata taacttcgta taatgtatgc
     6841 tatacgaagt tataaggagt ttggcgcccg ttttttcgag ccccacacgt ttcggtgagt
     6901 atgagcggcg gcagattcga gcgtttccgg tttccgcggc tggacgagag cccatgatgg
     6961 gggctcccac caccagcaat cagggccctg attacacacc cacctgtaat gtcatgctgt
     7021 tcatcgtggt taatgctgct gtgtgctgtg tgtgtgtgtt gtttggcgct cattgttgcg
     7081 ttatgcagcg tacaccacaa tattggaagc ttattagcct ttctattttt tcgtttgcaa
     7141 ggcttaacaa cattgctgtg gagagggatg gggatatgga ggccgctgga gggagtcgga
     7201 gaggcgtttt ggagcggctt ggcctggcgc ccagctcgcg aaacgcacct aggacccttt
     7261 ggcacgccga aatgtgccac ttttcagtct agtaacgcct tacctacgtc attccatgca
     7321 tgcatgtttg cgcctttttt cccttgccct tgatcgccac acagtacagt gcactgtaca
     7381 gtggaggttt tgggggggtc ttagatggga gctaaaagcg gcctagcggt acactagtgg
     7441 gattgtatgg agtggcatgg agcctaggtg gagcctgaca ggacgcacga ccggctagcc
     7501 cgtgacagac gatgggtggc tcctgttgtc caccgcgtac aaatgtttgg gccaaagtct
     7561 tgtcagcctt gcttgcgaac ctaattccca attttgtcac ttcgcacccc cattgatcga
     7621 gccctaaccc ctgcccatca ggcaatccaa ttaagctcgc attgtctgcc ttgtttagtt
     7681 tggctcctgc ccgtttcggc gtccacttgc acaaacacaa acaagcatta tatataaggc
     7741 tcgtctctcc ctcccaacca cactcacttt tttgcccgtc ttcccttgct aacacaaaag
     7801 tcaagaacac aaacaaccac cccaaccccc ttacacacaa gacatatcta cagcaatggg
     7861 taaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     7921 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcaatgt
     7981 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     8041 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     8101 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     8161 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg caatggatgc
     8221 cattgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     8281 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     8341 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     8401 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     8461 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     8521 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     8581 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     8641 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     8701 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     8761 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     8821 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     8881 ataatcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
     8941 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
     9001 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc
     9061 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgata
     9121 acttcgtata atgtatgcta tacgaagtta tcagcatcgt aatagcctcc aagagattga
     9181 tcatcactct gaatgtacaa gcaacccaag tacctgctcc tgcacctaag ttcgtcaaaa
     9241 tcggttttac tcagagaatc aacaacccta ccaactgtac atactgctaa ccctgattct
     9301 ttgaataacc ccaataaggc tcctgctgac ccttctgccg ttctaggaaa ccagcttgtt
     9361 gagtacgctg tgaacatcac tcttggtata cccggtcagc ccttctcagt tcagattgac
     9421 acaggctcgt ctgacttgtg ggtgaagagt gacggctcct ccggtgcatt caacaagaag
     9481 gcttcttcta cttttcagga ggacgttccc aacggctttg caattgccca cggagacaaa
     9541 acctctgcca ttggagattg ggtcaaagat accatcaata tcggtggtgt tagtattgac
     9601 cagtatagta ttgaccagta tgagttcgcc atggctactc agacaaatac tgacccggtt
     9661 tttggtatcg gctacccgag caacgaggcg tcttatgatc gcgtgcattc gcggccgcat
     9721 ttaaatcccc acttcagaag ttcctatact ttctagagaa taggaacttc actatagagt
     9781 cgaataaggg cgacacccca taattagccc gggcgaaagg cccagtcttt cgactgagcc
     9841 tttcgtttta tttgatgcct ggcagttccc tactctcgca tggggagtcc ccacactacc
     9901 atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga ccaccgcgct
     9961 actgccgcca ggcaaacaag gggtgttatg agccatattc aggtataaat gggctcgcga
    10021 taatgttcag aattggttaa ttggttgtaa cactgacccc tatttgttta tttttctaaa
    10081 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10141 gaaaaaggaa gaatatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10201 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10261 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    10321 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    10381 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10441 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10501 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10561 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10621 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10681 gtgacaccac gatgcctgta gcgatggcaa caacgttgcg caaactatta actggcgaac
    10741 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tccggagccg
    10861 gtgagcgtgg ttctcgcggt atcatcgcag cgctggggcc agatggtaag ccctcccgta
    10921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10981 ctgagatagg tgcctcactg attaagcatt ggtaagcaga gcattacgct gacttgacgg
    11041 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgcgcgt cgttccactg
    11101 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    11161 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    11221 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    11281 tgttcttcta gtgtagccgt agttagccca ccacttcaag aactctgtag caccgcctac
    11341 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    11401 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    11461 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    11521 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    11581 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    11641 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    11701 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    11761 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    11821 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    11881 cgagtcagtg agcgaggaag cggaaggcga gagtagggaa ctgccaggca tcaaactaag
    11941 cagaaggccc ctgacggatg gcctttttgc gtttctacaa actctttctg tgttgtaaaa
    12001 cgacggccag tcttaagctc gggccccctg ggcggttctg ataacgagta atcgttaatc
    12061 cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga
    12121 gcatttgtca gaatatttaa gggcgcctgt cactttgctt gatatatgag aattatttaa
    12181 ccttataaat gagaaaaaag caacgcactt taaataagat acgttgcttt ttcgattgat
    12241 gaacacctat aattaaacta ttcatctatt atttatgatt ttttgtatat acaatatttc
    12301 tagtttgtta aagagaatta agaaaataaa tctcgaaaat aataaaggga aaatcagttt
    12361 ttgatatcaa aattatacat gtcaacgata atacaaaata taatacaaac tataagatgt
    12421 tatcagtatt tattatgcat ttagaataaa ttttgtgtcg cccttattcg actcactata
    12481 gaagttccta ttctctagaa agtataggaa cttcacttca ttttatttaa atttgcggcc
    12541 gc
//
