LOCUS       sequence-206191-        2697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC28
FEATURES             Location/Qualifiers
     source          1..2697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..56
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(620..638)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        706..810
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1032..1823
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1994..2582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2483..2502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
       61 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatcac
      121 tctgtggtct caaatggctt cgaaaaggat cttgaaagag ctcaaggatc tccagaagga
      181 tcctccaact tcctgcagtg ctggcccagt tgctgaggac atgtttcatt ggcaagctac
      241 aataatgggt ccatctgata gtccttattc aggcggtgtc tttctcgtaa ccattcactt
      301 ccctccggat tatcctttca aaccaccaaa ggttgcattc aggacaaaag tgttccaccc
      361 taatgtcaac agcaatggaa gcatttgcct tgacattttg aaagaacaat ggagtcctgc
      421 actcaccata tcgaaggttt tgctttcgat atgttcattg ttaacggacc caaacccaga
      481 tgatccattg gttccagaga ttgctcacat gtacaaaacc gatagagcaa agtatgagtc
      541 tactgcgaga agctggactc agaaatatgc aatgggatga gctttgagac cacgaagtgg
      601 ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      661 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      721 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      781 atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg
      841 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      901 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
      961 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     1021 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     1081 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     1141 cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa
     1201 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     1261 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     1321 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     1381 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     1441 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     1501 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     1561 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     1621 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     1681 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     1741 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga
     1801 tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg
     1861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagt
//
