LOCUS       sequence-206192-       11990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HOXA10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Sugimura R, Jha DK, Han A, Soria-Valles C, da Rocha EL, Lu YF, 
            Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong I, Sousa P, Zhu 
            TN, Ditadi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley 
            GQ
  TITLE     Haematopoietic stem and progenitor cells from human pluripotent stem
            cells.
  JOURNAL   Nature. 2017 May 17. doi: 10.1038/nature22370.
  PUBMED    28514439
REFERENCE   2  (bases 1 to 11990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 May 17. doi: 10.1038/nature22370."
COMMENT     Sequence Label: pINDUCER-21-HOXA10
FEATURES             Location/Qualifiers
     source          1..11990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(70..88,89..111,112..130,131..153,154..172,173..195,
                     196..214,215..237,238..256,257..279,280..298,299..321,
                     322..340)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(112..130,131..153,154..172,173..195,196..214,215..237,
                     238..256,257..279,280..298,299..321,322..340,341..363,
                     364..382)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(154..172,173..195,196..214,215..237,238..256,257..279,
                     280..298,299..321,322..340,341..363,364..382,383..405,
                     406..424)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        457..495
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     492..516
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    625..649
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1838..1862)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1915..1941
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        2161..3339
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2392..3330
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3287..3307
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3409..3442
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(3449..4066,4067..4072,4073..4108,4109..4111,4112..4147,
                     4148..4150,4151..4186,4187..4195)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4248..4822
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4416..4433)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4643..4662
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4838..4840,4841..4843,4844..5557)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4838..4840,4841..4843,4844..5554)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4883..4904)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5144..5163)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5491..5512
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5693..5712
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6000..6588
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6053..6073)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6471..6482)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6663..6896
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7015..7033)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7101..7205
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7206..7274,7275..8066)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7424..7443)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8237..8825
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8726..8745
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8979..8996
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9236..9257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9272..9277,9278..9295,9296..9302)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9310..9326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9315..9337
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9334..9350
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9334..9350
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9400..9419
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9611..10244
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10291..10416
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10913..11146
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11331..11375
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11668..11785
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    11840..11858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11882..11900
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11924..11942
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11966..11984
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
       61 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      121 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      181 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      241 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      301 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      361 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      421 gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta ggcgtgtacg gtgggaggcc
      481 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
      541 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga actagtccag
      601 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc caccatgtca
      661 tgctcggaga gccccgccgc gaactctttt ttggtcgact cgctcatcag ctcgggcaga
      721 ggcgaggcag gcggcggtgg tggtggcgcg gggggcggcg gcggtggcgg ttactacgcc
      781 cacggcgggg tctacctgcc gcccgccgcc gacctgccct acgggctgca gagctgcggg
      841 ctcttcccca cgctgggcgg caagcgcaat gaggcagcgt cgccgggcag cggtggcggt
      901 ggcgggggtc taggtcccgg ggcgcacggc tacgggccct cgcccataga cctgtggcta
      961 gacgcgcccc ggtcttgccg gatggagccg cctgacgggc cgccgccgcc gccccagcag
     1021 cagccgccsc ccccgccgca accaccccag ccagcgccgc aggccacctc gtgctctttc
     1081 gcgcagaaca tcaaagaaga gagctcctac tgcctctacg actcggcgga caaatgcccc
     1141 aaagtctcgg ccaccgccgc cgaactggct cccttcccgc ggggcccgcc gcccgacggc
     1201 tgcgccctgg gcacctccag cggggtgcca gtgcctggct acttccgcct ttctcaggcc
     1261 tacggcaccg ccaagggcta tggcagcggc ggcggcggcg cgcagcaact cggggctggc
     1321 ccgttccccg cgcagccccc ggggcgcggt ttcgatctcc cgcccgcgct agcctccggc
     1381 tcggccgatg cggcccggaa ggagcgagcc ctcgattcgc cgccgccccc cacgctggct
     1441 tgcggcagcg gcgggggctc gcagggcgac gaggaggcgc acgcgtcgtc ctcggccgcg
     1501 gaggagctct ccccggcccc ttccgagagc agcaaagcct cgccggagaa ggattccctg
     1561 ggcaattcca aaggtgaaaa cgcagccaac tggctcacgg caaagagtgg tcggaagaag
     1621 cgctgcccct acacgaagca ccagacactg gagctggaga aggagtttct gttcaatatg
     1681 taccttactc gagagcggcg cctagagatt agccgcagcg tccacctcac ggacagacaa
     1741 gtgaaaatct ggtttcagaa ccgcaggatg aaactgaaga aaatgaatcg agaaaaccgg
     1801 atccgggagc tcacagccaa ctttaatttt tccttggacc cagctttctt gtacaaagtg
     1861 gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaataccca
     1921 tacgacgtcc cagactacgc ttagtaatga ttaattaaac tagaaattct accgggtagg
     1981 ggaggcgctt ttcccaaggc agtctggagt aacccaccca agatctggcc tccgcgccgg
     2041 gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gccgcgtaag atctggcctc
     2101 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc ctagtcgtga
     2161 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     2221 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     2281 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     2341 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     2401 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     2461 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2521 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2581 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2641 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2701 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2761 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     2821 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     2881 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     2941 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3001 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3061 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3121 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3181 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3241 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3301 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccgcca tgctagtaaa
     3361 ttgtccgcta aattctggcc gtttttggct tttttgttag acggatccat aacttcgtat
     3421 aatgtatgct atacgaagtt atgccaccat gtctagactg gacaagagca aagtcataaa
     3481 cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     3541 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     3601 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
     3661 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct
     3721 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     3781 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     3841 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
     3901 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     3961 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
     4021 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc
     4081 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
     4141 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag
     4201 taaggatccg cggccgcact agaggaattc cgcccctctc cccccccccc ctctccctcc
     4261 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     4321 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     4381 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     4441 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     4501 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     4561 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     4621 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     4681 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     4741 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     4801 cctttgaaaa acacgatgat aagcttaccg gtccaccatg gtgagcaagg gcgaggagct
     4861 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     4921 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     4981 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     5041 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     5101 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     5161 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     5221 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     5281 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     5341 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     5401 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     5461 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     5521 cgggatcact ctcggcatgg acgagctgta caagtaagca tgcccgacgg cgaggatctc
     5581 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     5641 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     5701 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     5761 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     5821 tgatgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     5881 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     5941 tgcaattgtt gttgtttagt ccctcccaat tcgatatcaa gcttatcgcg atagatccta
     6001 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6061 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6121 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6181 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6241 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6301 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6361 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     6421 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6481 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6541 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt
     6601 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6661 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6721 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6781 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6841 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6901 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6961 tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg
     7021 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     7081 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7141 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7201 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7261 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7321 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7381 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7441 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7501 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7561 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7621 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7681 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7741 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7801 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7861 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7921 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7981 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8041 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8101 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8161 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8221 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8281 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8341 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8401 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8461 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8521 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8581 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8641 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8701 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8761 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8821 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8881 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8941 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9001 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9061 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9121 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9181 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     9241 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     9301 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
     9361 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9421 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     9481 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     9541 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     9601 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     9661 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     9721 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     9781 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     9841 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     9901 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     9961 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
    10021 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
    10081 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    10141 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    10201 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
    10261 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
    10321 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
    10381 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
    10441 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
    10501 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
    10561 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
    10621 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
    10681 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
    10741 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
    10801 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
    10861 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
    10921 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
    10981 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
    11041 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
    11101 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
    11161 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
    11221 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    11281 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    11341 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    11401 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    11461 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    11521 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
    11581 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa
    11641 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
    11701 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
    11761 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
    11821 gactaggatc ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
    11881 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
    11941 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
//
