LOCUS       sequence-206193-        2697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC29
FEATURES             Location/Qualifiers
     source          1..2697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..43
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(607..625)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        693..797
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1019..1810
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1981..2569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2470..2489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
       61 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct gtggtctcaa
      121 atggcaacga gacggatatt gaaagaattg aaggagttgc aaagggaccc tcctgtatca
      181 tgcagtgcag gtccaacagg agaagatatg ttccactggc aagctactat aatgggtccg
      241 aatgaaagtc cttactccgg aggtgttttc cttgtcaata ttcatttccc tcctgattat
      301 ccttttaaac ctcccaaggt tgtattcaga accaaagtgt ttcacccaaa catcaacagt
      361 aatggaaaca tatgtttgga cattctcaaa gaccaatgga gccctgccct taccatttct
      421 aaggtgcttc tctctatatg ctctcttcta acagatccaa accctgacga ccctctggtg
      481 ccagagatag ctcacatata caaaactgac aagaccaaat atgaagccat ggctcgaagc
      541 tggacccaga agtatgcctt gttttaagct ttgagaccac gaagtggctc ttcagtggac
      601 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      661 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      721 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      781 attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      841 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca
      901 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
      961 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     1021 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     1081 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     1141 cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     1201 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     1261 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     1321 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     1381 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     1441 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     1501 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     1561 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     1621 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     1681 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     1741 gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt
     1801 cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     1861 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     1921 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1981 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2041 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2101 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2161 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2221 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2281 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2341 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2401 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2461 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2521 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2581 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2641 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgc
//
