LOCUS       sequence-206195-        2715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC31
FEATURES             Location/Qualifiers
     source          1..2715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..60
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(642..660)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        728..832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1054..1845
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2016..2604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2505..2524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
       61 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      121 tcactctgtg gtctcaaatg tttaagaaaa tggataaaaa agcagcgcag agaattgcga
      181 tggaatacag agctatgatc tcgaaagaat ctttgttcag tattggtcaa aactcgaaca
      241 atatatacga atggactgca gtgatccggg gtccagatgg cactccctat gaaggtggca
      301 tgtttaatct cagtattaag tttcctacgg attatccttt taaaccaccc aagtttacgt
      361 ttaaaactcc gatttaccat ccaaatatca atgatgaagg atcgatttgc atgaacattc
      421 ttaaagacaa atggactcct gctcttatgg ttgaaaaggt gcttctgtca atacttttac
      481 tattggaaaa accaaaccca gatgatcctc ttgtacctga aattggacag ctcttcaaga
      541 ataacagatt ccaatttgac cagagagctc gagaattcac tgctcgacat gctaataatt
      601 aagctttgag accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt
      661 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      721 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      781 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc
      841 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
      901 catggcttgt tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag
      961 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     1021 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta
     1081 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     1141 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat
     1201 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     1261 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     1321 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     1381 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     1441 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     1501 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     1561 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     1621 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     1681 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     1741 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     1801 gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt
     1861 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1921 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1981 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2041 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2101 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2161 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2221 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2281 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2341 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2401 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2461 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2521 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2581 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2641 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2701 cgtattaccg ccttt
//
