LOCUS       sequence-206197-        3180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC32
FEATURES             Location/Qualifiers
     source          1..3180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..39
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1086..1104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1172..1276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1498..2289
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2460..3048
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2949..2968
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
       61 cgcaaaccgc ctctccccgc gcgttggccg attcattaat cactctgtgg tctcaaatgg
      121 cggatgagag gtataatcgg aagaatccgg cggtgaagag gattttgcag gaggttaagg
      181 agatgcaagc taatccgtct gatgatttta tgtctcttcc tcttgaggag aatatctttg
      241 agtggcaatt cgccatccga ggtcctggtg atactgaatt cgagggcggg atttatcatg
      301 ggagaattca gttgcctgca gattatccat tcaaacctcc ttcattcatg ttattgaccc
      361 ctaacgggcg ctttgaaact aacaccaaga tttgcttgag catttcaaac taccaccctg
      421 agcattggca accttcatgg agtgttcgga ctgctttggt ggctctcatt gcatttatgc
      481 ccacaagtcc caatggagca ctgggctcag tagattatcc aaaggatgag agacgtacac
      541 tagccattaa atcacgtgag acaccaccta agtatggctc tcctgaacgg caaaaaatta
      601 ttgatgagat tcatcagtac atccttagca aagcaaccgt ggttccaaaa cctcttcctc
      661 tggaatgtag ccaagcacct tccatcgtat cagaagctca ctcccaagtt gagccacagg
      721 aagcgataac tgtagtagaa gagcggtcca tcgctacaac agacaccata gttgatgatc
      781 aaatcataga agagacagct gaagcagtca atacagcagc tagtgtggtt cccgctgcag
      841 cacctttacc agcggttgaa gttgtggtca aagcttctgt aagtggtgag caaaggatgg
      901 ccagaagagc ggctcagaag ccagtcgatg acagactctt cacgtgggcg gcggttgggc
      961 tcacaatcgc tatcatggtt cttcttctga agaagttcat aaaatcaaat ggttacagta
     1021 ctgggtttat ggatgatcag tcttgagctt tgagaccacg aagtggctct tcagtggacg
     1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1261 ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     1321 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttt tggggtacag
     1381 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     1441 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     1501 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     1561 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     1621 ctgaagccac acagcgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     1681 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     1741 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     1801 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     1861 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     1921 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     1981 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2041 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     2101 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     2161 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     2221 cgcgcagatc agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc
     2281 ggcaaataac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2341 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2401 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2461 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2521 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2581 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2641 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2701 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2761 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2821 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2881 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2941 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3001 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3061 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
//
