LOCUS       sequence-206201-        2712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC35
FEATURES             Location/Qualifiers
     source          1..2712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     51..68
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(647..665)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        733..837
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1059..1850
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2021..2609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2510..2529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
       61 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      121 ttcattaatc actctgtggt ctcaaatggc caacagtaat ttgccgcgaa gaatcatcaa
      181 ggaaactcaa cgtctgctca gtgaaccggc tcccggtata agtgcctctc catctgagga
      241 taatatgcgg tatttcaacg ttatgattct tggtcctaca caatcacctt atgaaggagg
      301 agttttcaag ttggagctct ttttgcctga agaataccct atggcagctc ccaaggttag
      361 gtttctcaca aagatatacc atcctaacat tgacaagctt ggaagaatct gtcttgatat
      421 tctcaaggac aaatggagcc ctgcactaca aatacgaaca gtgctcttaa gtattcaagc
      481 tcttctgagt gcaccaaacc cagatgatcc gctttctgag aacattgcta agcactggaa
      541 gagtaatgag gcagaagctg tggatacagc taaggaatgg acccgtcttt atgcaagcgg
      601 cgcatgagct ttgagaccac gaagtggctc ttcagtggac gaaagggcct cgtgatacgc
      661 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      721 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      781 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      841 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
      901 gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct cgggcatcca
      961 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     1021 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg
     1081 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     1141 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata
     1201 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     1261 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     1321 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     1381 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     1441 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     1501 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     1561 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     1621 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     1681 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     1741 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     1801 aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc
     1861 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1921 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1981 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2041 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2101 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2161 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2221 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2281 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2341 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2401 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2461 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2521 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2581 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2641 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2701 ataaccgtat ta
//
