LOCUS       sequence-206204-        3480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC37
FEATURES             Location/Qualifiers
     source          1..3480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..90
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1437..1455)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1523..1627
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1849..2640
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2811..3399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3300..3319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
       61 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      121 cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaaatg gctcaagcag
      181 cgagattaag cctgagaatg cagaaagagc taaagcttct cctctccgat ccacctcacg
      241 gcgcttcgtt tccacatctc tcttccgccg ccaatggctc cggcgatttc tccactttct
      301 ccaccatcga tgctcaaatc gaaggtcctg aagatactgt atatgctaat ggaatcttca
      361 atttgaagat tcaaatccct gagagatatc catttcagcc accaattgtg tcgttcgcta
      421 caccgattta tcacccaaac atcgataaca gtggccggat ttgccttgac attctaaatc
      481 tccctcccaa gggagcttgg caaccatcgt tgaatatctc gacagttttg acgagtatga
      541 gattgttgct tagtgaacca aatcctgatg atggattgat gtgtgaagta agcagggagt
      601 ataaatacaa tagacaaact tttgattata aggcacgaga gatgactgag aagtatgcta
      661 aggttaaagc tgatggatgc agcaccagct tacaaattaa gaatcatgga gatgagaagt
      721 ctggtgaaag tggaaatagt gttaagctta agttgacagt ggaatcttct ttaagcatag
      781 cacatacagt gaaacgtgag actgcaggga gagatgaaca agaggatggc aatgggaaaa
      841 gaaaagcagt ggttggtttt ggcgagggga attcgtttgg aaacgatggg attaaaagga
      901 gtaggaagaa gttatctctt gcattgcctt ctcaatctca aaagaaagac ttgtgtggtg
      961 aagaagagct cacacgtggt gtttcagctg cttgcaaaga aaacaaaaaa ccaaatttga
     1021 atggaaagaa gctttctctg gggctgaagc agccatgtaa tgatactttg gcgagttttc
     1081 gaacttctgc tgcgaagagt gacaacaata gactaagccg aaaactgtcc ctgagatcac
     1141 cacttggaga gttgaatgaa gttagcaaac ctgaggtact tgcgcaaact gatatgaaac
     1201 tggagatgaa tcaaaatgaa gatgcaagaa gtttgcgagg tgagtttgag aattcggtat
     1261 tggaagaaac atcaatggct gaatccattg ttgttctaga cagtgatgat agtggacaag
     1321 aagaagaaga aagagtctct tcttcgaggt caagattatt actggcaaag aggagagtcc
     1381 tcaaatgtag accttgagct ttgagaccac gaagtggctc ttcagtggac gaaagggcct
     1441 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     1501 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1561 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1621 gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     1681 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct
     1741 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     1801 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg
     1861 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     1921 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     1981 cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     2041 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     2101 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     2161 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     2221 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     2281 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     2341 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     2401 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     2461 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     2521 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     2581 cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa
     2641 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2701 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2761 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2821 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2881 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2941 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3001 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3061 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3121 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3181 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3241 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3301 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3361 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3421 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
//
