LOCUS       sequence-206205-        2727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-135
FEATURES             Location/Qualifiers
     source          1..2727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     57..74
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(668..686)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        754..858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1080..1871
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2042..2630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2531..2550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
       61 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      121 ggccgattca ttaatcactc tgtggtctca aatgagttcg gaggaagcca aggtcgttgt
      181 tccgaggaac tttagattgt tggaagagct tgaaagaggt gagaaaggta ttggagatgg
      241 tactgttagc tatggaatgg atgatgctga tgatatttat atgcaatctt ggactggcac
      301 cattcttggt cctcctaata ctgcatatga agggaaaatc tttcagctga agcttttctg
      361 tggtaaggag taccctgaaa gtccaccgac tgtgaggttc cagacccgga taaacatggc
      421 ttgtgttaac ccagaaactg gagtggttga accgagtctc ttccctatgc tcactaactg
      481 gcggcgagaa tatacaatgg aggacatact ggttaagctg aaaaaagaaa tgatgacttc
      541 gcataaccgg aagttagctc aacccccgga aggtaacgag gaagctaggg cagatccaaa
      601 aggaccagct aaatgttgtg tgatgtgagc tttgagacca cgaagtggct cttcagtgga
      661 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      721 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      781 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      841 tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      901 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac
      961 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     1021 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     1081 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     1141 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     1201 gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     1261 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     1321 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     1381 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     1441 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     1501 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     1561 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     1621 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     1681 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     1741 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     1801 cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag
     1861 tcggcaaata actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1921 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1981 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2041 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2101 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2161 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2221 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2281 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2341 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2401 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2461 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2521 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2581 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2641 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2701 cgttatcccc tgattctgtg gataacc
//
