LOCUS       sequence-206207-        2730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-136
FEATURES             Location/Qualifiers
     source          1..2730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..80
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(677..695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        763..867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1089..1880
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2051..2639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2540..2559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
       61 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
      121 cgcgttggcc gattcattaa tcactctgtg gtctcaaatg ggttcggaag aagagaaggt
      181 cgttgttccc agaaacttta gattattgga agagctagaa agaggtgaga aaggaattgg
      241 agatgggaca gtgagttatg gaatggatga tgctgatgat attttgatgc aatcttggac
      301 tggtaccatt cttggtcctc ataatactgc gtatgaaggg aaaatctttc agctgaagct
      361 attctgtgga aaggattatc cggaaagtcc acctactgtg aggtttcaga gtcgtataaa
      421 catggcttgt gtcaatcctg agaatggagt ggttgatccg agtcacttcc ctatgctttc
      481 caactggaga agagaattca caatggagga tctactgatt cagcttaaaa aagaaatgat
      541 gtcatcgcag aaccgcaagt tagctcaacc cttggaaggt aatgaggaag gcaggacaga
      601 cccaaaggga ctagtggtga aatgttgcgt gatgtgagct ttgagaccac gaagtggctc
      661 ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      721 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      841 cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc
      901 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
      961 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     1021 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     1081 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     1141 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     1201 tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc
     1261 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     1321 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     1381 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     1441 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     1501 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     1561 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     1621 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     1681 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     1741 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     1801 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca
     1861 ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt
     1921 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1981 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2041 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2101 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2161 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2221 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2281 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2341 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2401 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2461 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2521 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2581 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2641 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2701 tctttcctgc gttatcccct gattctgtgg
//
