LOCUS       sequence-206208-        2691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-137
FEATURES             Location/Qualifiers
     source          1..2691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..55
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(613..631)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        699..803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1025..1816
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1987..2575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2476..2495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
       61 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
      121 ctgtggtctc aaatgactct tggctcagga tcgagtgttg tcgttccgag gaatttccgg
      181 ttgctggagg agcttgaacg tggagagaaa ggtattggag atggaactgt gagctatgga
      241 atggatgatg gagatgacat ttatatgcgc tcttggactg gcactatcat cggtcctcac
      301 aacgtaactg tacatgaggg tcggatttat cagttgaagc tcttctgtga caaagattac
      361 cctgagaaac ctccgactgt tcggttccat tcgcgtatca acatgacttg tgtcaaccat
      421 gataccggcg tggtggattc aaagaagttt ggggttcttg caaactggca aagacagtac
      481 acaatggagg acatactgac tcaattgaag aaggagatgg ctgcatcgca taaccggaag
      541 cttgttcaac ctcccgaagg aactttcttc taagctttga gaccacgaag tggctcttca
      601 gtggacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      661 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      721 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      781 aataatattg aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac
      841 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg
      901 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
      961 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     1021 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     1081 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     1141 tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga
     1201 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     1261 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     1321 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     1381 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     1441 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     1501 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     1561 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     1621 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     1681 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     1741 ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa
     1801 ggtagtcggc aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1861 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1921 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1981 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2041 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2101 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2161 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2221 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2281 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2341 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2401 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2461 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2521 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2581 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2641 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt g
//
