LOCUS       sequence-206213-        3558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-21
FEATURES             Location/Qualifiers
     source          1..3558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1827..1845)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1913..2017
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2239..3030
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3201..3558,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactctg tggtctcaaa
      481 tggatcaaga gatagagatt ccttccttct tcctttgtcc aatctctcta gatatcatga
      541 aagatccggt gatagtttcc accggaataa cctacgaccg agaaagcatc gagaagtggc
      601 tcttttccgg taagaaaaac tcatgtccag tcaccaaaca agtcataacc gaaactgatc
      661 ttaccccaaa ccacactctt cgccgtttga ttcaatcttg gtgtaccctc aacgcatcat
      721 atggtataga gaggatccca actccaaaac ctccgatctg taaatcggag atcgaaaagc
      781 ttatcaaaga gtcgtcatct tcgcatctaa accaagtcaa atgcctcaaa cgacttcgtc
      841 aaatagtatc tgagaataca accaacaaac ggtgcttaga agccgcagaa gttccggagt
      901 ttttggcaaa tatcgtaagc aactctgtag atacgtataa ctctccttct tcttcacttt
      961 cttcttcgaa tctcaacgac atgtgtcagt caaacatgtt ggagaatcga tttgattctt
     1021 caagaagctt gatggacgaa gccctaagcg tactctacca tcttgacaca tcggagacag
     1081 ccctcaagag tcttttaaac aacaagaaag gaacaaatct tgtgaaaacg ttgacgaaga
     1141 ttatgcaacg tgggatctac gagtcaagag cctatgcggc tttgcttctc aagaagctcc
     1201 tggaagttgc ggatccaatg cagatcatat tgttggaacg agaacttttc ggtgaggtga
     1261 ttcaaatctt gcacgaccag atctctcaca aggcgacaag atcagcaatg caaatcttgg
     1321 tgatcacatg tccatgggga aggaatagac acaaggcagt ggaaggtgga acaatctcga
     1381 tgataatcga gcttttaatg gacgatactt tctcatcaga gagaaggaat tcagagatgg
     1441 cgatggtggt tcttgatatg ttatgtcagt gcgcagaagg aagagctgag ttcttgaatc
     1501 acggtgccgc tattgcggtt gtgtctaaga agatattgag ggtatcacaa ataactagtg
     1561 aaagggcagt tagggttttg ctttcggttg ggaggttctg cgcgacaccc tctttgttgc
     1621 aggagatgtt gcaattggga gttgtagcaa agctgtgtct ggtacttcaa gttagctgtg
     1681 gtaacaagac taaagaaaag gctaaagaat tgcttaagct tcacgctagg gtttggaggg
     1741 aatcaccttg tgtcccaaga aatttgtatg attcgtatcc tgcttgagct ttgagaccac
     1801 gaagtggctc ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     1861 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     1921 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1981 ctgataaatg cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag
     2041 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     2101 ctgttttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     2161 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     2221 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     2281 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     2341 ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg
     2401 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     2461 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     2521 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     2581 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     2641 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     2701 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     2761 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     2821 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     2881 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     2941 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa
     3001 ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt
     3061 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     3121 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3541 cccagcttgg agcgaacg
//
