LOCUS       sequence-20624-e         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG416GAL-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(125..249)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(326..347)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(587..606)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttcgtttcgg ggagagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
       61 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt
      121 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
      181 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta
      241 caaagtggtg atgggctgca ggaattcgat atcaagctta atggtgagca agggcgagga
      301 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      361 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      421 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
      481 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      541 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      601 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaac
//
