LOCUS       sequence-206248-        3558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-22
FEATURES             Location/Qualifiers
     source          1..3558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     78..95
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1520..1538)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1606..1710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1932..2723
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2894..3482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3383..3402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
       61 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      121 aaccgcctct ccccgcgcgt tggccgattc attaatcact ctgtggtctc aaatggatca
      181 agagatagag attccttcct tcttcctttg tccaatctct ctagatatca tgaaagatcc
      241 ggtgatagtt tccaccggaa taacctacga ccgagaaagc atcgagaagg cgctcttttc
      301 cggtaagaaa aactcatgtc cagtcaccaa acaagtcata accgaaactg atcttacccc
      361 aaaccacact cttcgccgtt tgattcaatc ttggtgtacc ctcaacgcat catatggtat
      421 agagaggatc ccaactccaa aacctccgat ctgtaaatcg gagatcgaaa agcttatcaa
      481 agagtcgtca tcttcgcatc taaaccaagt caaatgcctc aaacgacttc gtcaaatagt
      541 atctgagaat acaaccaaca aacggtgctt agaagccgca gaagttccgg agtttttggc
      601 aaatatcgta agcaactctg tagatacgta taactctcct tcttcttcac tttcttcttc
      661 gaatctcaac gacatgtgtc agtcaaacat gttggagaat cgatttgatt cttcaagaag
      721 cttgatggac gaagccctaa gcgtactcta ccatcttgac acatcggaga cagccctcaa
      781 gagtctttta aacaacaaga aaggaacaaa tcttgtgaaa acgttgacga agattatgca
      841 acgtgggatc tacgagtcaa gagcctatgc ggctttgctt ctcaagaagc tcctggaagt
      901 tgcggatcca atgcagatca tattgttgga acgagaactt ttcggtgagg tgattcaaat
      961 cttgcacgac cagatctctc acaaggcgac aagatcagca atgcaaatct tggtgatcac
     1021 atgtccatgg ggaaggaata gacacaaggc agtggaaggt ggaacaatct cgatgataat
     1081 cgagctttta atggacgata ctttctcatc agagagaagg aattcagaga tggcgatggt
     1141 ggttcttgat atgttatgtc agtgcgcaga aggaagagct gagttcttga atcacggtgc
     1201 cgctattgcg gttgtgtcta agaagatatt gagggtatca caaataacta gtgaaagggc
     1261 agttagggtt ttgctttcgg ttgggaggtt ctgcgcgaca ccctctttgt tgcaggagat
     1321 gttgcaattg ggagttgtag caaagctgtg tctggtactt caagttagct gtggtaacaa
     1381 gactaaagaa aaggctaaag aattgcttaa gcttcacgct agggtttgga gggaatcacc
     1441 ttgtgtccca agaaatttgt atgattcgta tcctgcttga gctttgagac cacgaagtgg
     1501 ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1561 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1621 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1681 atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg
     1741 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     1801 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     1861 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     1921 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     1981 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     2041 cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa
     2101 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     2161 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     2221 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     2281 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     2341 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     2401 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     2461 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     2521 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     2581 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     2641 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga
     2701 tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg
     2761 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2821 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2881 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2941 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3001 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3061 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3121 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3181 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3241 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3301 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3361 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3421 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3481 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3541 tgttctttcc tgcgttat
//
