LOCUS       sequence-206252-        7044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose inducible VioB-mCherry expression (weaker RBS). Used to 
            test burden response in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, 
            Awan AR, Gilbert C, Stan GB, Ellis T
  TITLE     Burden-driven feedback control of gene expression.
  JOURNAL   Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635.
  PUBMED    29578536
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635."
COMMENT     Sequence Label: pSB1C3-H3
FEATURES             Location/Qualifiers
     source          1..7044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..58
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(82..99)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(253..841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(333..352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1024..1118)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1139..1798)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1713..1732
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1960..1978
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1982..2025)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2028..2049
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(2050..2928)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2955..3239
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3162..3181
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(6479..6497)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6747..6766
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7024..7044
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
       61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
     1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
     1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
     2041 cttctagagt tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
     2101 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
     2161 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
     2221 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
     2281 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
     2341 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
     2401 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
     2461 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
     2521 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
     2581 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
     2641 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
     2701 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
     2761 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
     2821 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
     2881 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
     2941 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt
     3001 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta
     3061 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag
     3121 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
     3181 cataagatta gcggatccta cctgacactt tttatcgcaa ctctctataa tttctccata
     3241 cccgtttttt tgggctagca ttacccgcat cacaaggaaa agcccaatat gagcattctg
     3301 gatttcccgc gtatccactt ccgtggctgg gcccgtgtca atgcgccgac cgcgaaccgc
     3361 gatccgcacg gccacatcga tatggccagc aataccgtgg cgatggcggg tgagccgttc
     3421 gacctggcac gccatcctac ggagttccac cgtcacctgc gctccctggg tccgcgcttc
     3481 ggcttggatg gtcgtgctga cccggaaggc ccgttcagcc tggccgaggg ctacaacgct
     3541 gccggtaaca accacttttc gtgggagagc gcaaccgtta gccacgtgca atgggatggc
     3601 ggtgaggcgg atcgtggtga cggtctggtc ggtgctcgtt tggcactgtg gggtcactac
     3661 aatgattatc tgcgtaccac cttcaatcgt gctcgttggg tcgacagcga cccgacgcgc
     3721 cgtgacgctg cacaaatcta tgcgggccaa ttcaccatta gcccggctgg tgccggtccg
     3781 ggtacgccgt ggctgtttac ggcagacatt gatgatagcc atggtgcacg ttggacgcgt
     3841 ggcggccaca ttgcagagcg tggcggccac ttcttggatg aagagtttgg tctggcacgc
     3901 ctgtttcagt tctctgtgcc gaaagatcac ccacattttc tgtttcaccc gggtccgttt
     3961 gattccgagg cctggcgtcg tctgcaattg gctctggagg atgacgacgt tctgggtctg
     4021 accgtgcaat atgcgttgtt caatatgagc accccgcctc agccgaacag cccggttttt
     4081 cacgatatgg tcggtgttgt cggtctgtgg cgtcgtggtg aactggcgag ctacccggct
     4141 ggtcgtctgc tgcgtccgcg tcaaccgggt ctgggtgacc tgaccctgcg cgtcaacggt
     4201 ggtcgcgttg cgctgaattt ggcgtgtgcc attccgttca gcactcgtgc cgcgcagcca
     4261 agcgcaccgg accgcctgac cccggacctg ggtgccaaac tgccgctggg cgatctgctg
     4321 ctgcgtgatg aggacggcgc actgttggca cgtgtgccgc aggctctgta ccaagactat
     4381 tggacgaatc acggtattgt ggacctgccg ctgctgcgcg aaccgcgtgg tagcttgacc
     4441 ctgagcagcg aactggcgga gtggcgtgag caagactggg tcacccaaag cgacgcgtct
     4501 aacctgtacc tggaggcacc ggatcgccgt cacggtcgct ttttccctga gagcatcgcg
     4561 ctgcgcagct actttcgcgg tgaagcgcgt gcgcgtccgg atatcccgca tcgtatcgag
     4621 ggcatgggcc tggtcggcgt cgaatctcgt caggatggcg acgctgcgga atggcgtctg
     4681 acgggtctgc gtccgggtcc ggcacgcatt gttctggacg atggtgccga ggcgatccct
     4741 ctgcgtgttc tgcctgacga ttgggcgctg gatgacgcga ccgtcgaaga agtggattac
     4801 gcctttttgt accgccacgt tatggcgtat tacgagctgg tgtatccatt catgagcgac
     4861 aaggtgtttt ccctggctga tcgttgcaaa tgtgaaacgt acgcacgtct gatgtggcag
     4921 atgtgtgatc cgcagaaccg caacaagtcc tattacatgc cgagcacccg cgaactgtcg
     4981 gcaccgaaag ctcgtttgtt cttgaagtat ctggcccacg tggaaggcca ggcacgcctg
     5041 caagcacctc cgccagcggg tccggcacgc attgaatcta aagcccagtt ggcggcagag
     5101 ctgcgtaaag ccgtcgacct ggagctgtct gtgatgctgc aatacctgta cgcggcgtat
     5161 agcattccga actatgcaca gggccaacaa cgtgttcgtg acggtgcgtg gaccgccgag
     5221 cagctgcaac tggcgtgcgg tagcggtgac cgtcgccgtg atggcggtat tcgtgcagca
     5281 ctgctggaaa ttgctcatga agaaatgatt cattacctgg tcgttaacaa cctgctgatg
     5341 gccctgggcg agccgttcta cgcgggtgtc ccgctgatgg gcgaagcggc acgtcaggcg
     5401 tttggcctgg acaccgagtt cgctctggaa ccgtttagcg aaagcacgct ggcacgtttt
     5461 gttcgtctgg aatggccgca ctttatccca gcaccgggca aatccatcgc ggactgctat
     5521 gccgccattc gtcaggcgtt tttggatctg ccggacttgt ttggtggcga ggcaggtaag
     5581 cgtggcggtg aacaccacct gttcctgaat gagctgacca accgtgcgca tccgggttat
     5641 caactggaag ttttcgatcg cgactcggcg ctgtttggta ttgcatttgt gaccgatcag
     5701 ggcgaaggtg gcgctctgga cagcccgcac tacgaacata gccattttca acgtctgcgt
     5761 gaaatgagcg cgcgtatcat ggctcaaagc gcaccgttcg aaccggcgct gccggcgttg
     5821 cgtaatccgg ttctggatga gagcccgggt tgccaacgtg tcgcagacgg tcgtgcgcgt
     5881 gcgctgatgg cattgtacca aggcgtttat gagctgatgt ttgcgatgat ggcgcagcac
     5941 ttcgccgtga aaccgctggg tagcttgcgt cgcagccgcc tgatgaacgc agcaatcgat
     6001 ctgatgaccg gtctgttgcg tccgctgagc tgcgcgctga tgaacctgcc aagcggcatc
     6061 gccggtcgca cggccggtcc gccgctgccg ggtccggttg acacccgtag ctatgacgac
     6121 tacgcgctgg gctgtcgcat gctggcacgc cgttgcgagc gtctgctgga gcaggcgagc
     6181 atgctggaac cgggttggct gccggatgcg cagatggagc tgctggattt ctatcgtcgc
     6241 caaatgctgg acttggcgtg cggcaaactg agccgcgagg ccgccgaagc tgcagcaaag
     6301 gaggccgcag ctaaagaggc tgcagcgaaa gcgatggtga gcaagggcga ggaggataac
     6361 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     6421 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     6481 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     6541 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     6601 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     6661 gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa ggtgaagctg
     6721 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     6781 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     6841 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     6901 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     6961 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccacctaa
     7021 taatactagt agcggccgct gcag
//
