LOCUS       sequence-206254-        9707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose inducible expression of the Vibrio Fischery Lux operon. 
            Used to test burden response in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, 
            Awan AR, Gilbert C, Stan GB, Ellis T
  TITLE     Burden-driven feedback control of gene expression.
  JOURNAL   Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635.
  PUBMED    29578536
REFERENCE   2  (bases 1 to 9707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635."
COMMENT     Sequence Label: pSB1C3-Lux
FEATURES             Location/Qualifiers
     source          1..9707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..58
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(82..99)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(253..841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(333..352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1024..1118)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1139..1798)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1713..1732
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1960..1978
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1982..2025)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2028..2049
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(2050..2928)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2955..3239
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3162..3181
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    9687..9707
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
       61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
     1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
     1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
     2041 cttctagagt tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
     2101 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
     2161 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
     2221 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
     2281 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
     2341 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
     2401 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
     2461 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
     2521 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
     2581 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
     2641 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
     2701 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
     2761 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
     2821 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
     2881 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
     2941 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt
     3001 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta
     3061 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag
     3121 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
     3181 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
     3241 cccgtttttt tgggctagca gggaaaataa tgaataaatg tattccaatg ataattaatg
     3301 gaatgattca agattttgat aattatgcat ataaagaagt taaactaaat aatgataata
     3361 gagtaaaatt atctgtcatt actgaaagtt cagtttcaaa aacattaaat atcaaagata
     3421 gaattaatct aaatttaaat cagattgtga attttttata taccgttggt caacgatgga
     3481 aaagtgaaga atataatcgg cgacgaacct atatccgtga gttaaaaaca tatcttggtt
     3541 attctgatga aatggcaaga ttagaagcga attggattgc aatgttattg tgctctaaaa
     3601 gtgctttgta tgacattgtt aattatgatt tgggctctat acacgtatta gatgaatggc
     3661 ttccacgtgg tgattgctat gttaaagcac aaccgaaagg tgtttctgtt cacttgttag
     3721 ctggtaatgt tccattatca ggagtgacat ctattttgcg tgctatttta acaaaaaatg
     3781 agtgcattat taaaacttcg tcttcagatc cttttactgc aaacgcttta gtttccagtt
     3841 ttattgatgt taatgcagac catccaataa ccaaatcaat gtctgttatg tattggccgc
     3901 atgatgaaga tatgactcta tctcaaagaa taatgaatca tgccgacgtg gttattgctt
     3961 ggggtggaga cgaggcgatt aaatgggcgg taaaatattc accaccgcat gtcgatattc
     4021 tgaaatttgg accaaagaaa agcttaagta ttattgaagc tcctaaagat atagaagccg
     4081 cagcaatggg ggttgctcat gatatttgtt tctatgacca gcaagcctgc ttctctactc
     4141 aagacgttta ttatatagga gataatttac ctttattttt aaatgaactt gaaaaacagc
     4201 tagatcgata cgcgaaaatt ttaccaaaag gttcaaatag ttttgatgaa aaagcggcgt
     4261 ttactcttac tgaaaaagaa agtctatttg ctggatatga agtgagaaag ggagataagc
     4321 aagcttggtt aatagtcgta tcacctacaa atagctttgg aaatcaaccg ctatcacgaa
     4381 gtgtgtatgt tcatcaagta tctgatatta aagagataat tccttttgtt aataaaaata
     4441 gaacacaaac tgtttctatt tatccttggg aagcgtcatt aaaatatcga gataaattag
     4501 caagaagtgg agttgaaaga attgttgaat caggcatgaa taatattttc agagttggag
     4561 gggctcatga ttcattatct cctctccagt acctagttag gtttgtatcg catgagagac
     4621 catttaatta tacgacaaaa gatgttgcgg ttgaaatcga acaaacacgt tacttagagg
     4681 aagataagtt tttagttttt gtcccatagt taaaggaaat tatatgaaag atgaaagtgc
     4741 tttttttacg attgatcaca tcatcaagct tgataatggt cagtctatcc gagtttggga
     4801 aacactccct aaaaagaacg taccagagaa aaaacataca atacttattg cttcgggttt
     4861 tgctagaaga atggatcatt ttgcaggtct tgctgagtat ttatctacta acggttttca
     4921 tgtcattcgc tacgattctt tgcatcatgt tggattaagc agtggatgta taaatgaatt
     4981 tacgatgtcg attggaaaaa atagcctgct tacagtcgta gattggctta cagatcatgg
     5041 tgtcgaacga atagggctga ttgctgctag tttgtcagcg agaatcgcct atgaggtagt
     5101 aaataaaatt aaattatcat ttttaattac ggccgtaggt gtcgttaatc ttagagatac
     5161 attagaaaaa gcattggagt atgactattt gcaattacct atttcagagt taccagaaga
     5221 tcttgacttt gaaggtcata atttaggatc ggaggtcttt gttacagatt gctttaaaca
     5281 taattgggac acgttagact cgacacttaa tagtgttaaa ggattagcga ttccatttat
     5341 tgcttttact gcaaacgatg atagttgggt aaagcaaagt gaagttatag agctcattga
     5401 tagcattgaa tctagtaatt gtaagctcta ttcgctaatt ggaagttcac atgatcttgg
     5461 ggaaaatttg gttgtattaa gaaattttta tcaatcagtg acgaaggcag ccttagcatt
     5521 agatgatggt ttattggatt tagagataga cattattgaa cctcgatttg aggacgttac
     5581 aagtattact gttaaggagc gtagattaaa aaatgaaatt gaaaatgaat tattagaatt
     5641 ggcttaaata aacagaatca ccaaaaagga atagagtatg aagtttggaa atatttgttt
     5701 ttcgtatcaa ccaccaggtg aaactcataa gcaagtaatg gatcgctttg ttcggcttgg
     5761 tatcgcctca gaagaggtag ggtttgatac atattggacc ttagaacatc attttacaga
     5821 gtttggtctt acgggaaatt tatttgttgc tgcggctaac ctgttaggaa gaactaaaac
     5881 attaaatgtt ggcactatgg gggttgttat tccgacagca cacccagttc gacagttaga
     5941 agacgtttta ttattagatc aaatgtcgaa aggtcgtttt aattttggaa ccgttcgagg
     6001 gctataccat aaagattttc gagtatttgg tgttgatatg gaagagtctc gagcaattac
     6061 tcaaaatttc taccagatga taatggaaag cttacagaca ggaaccatta gctctgatag
     6121 tgattacatt caatttccta aggttgatgt atatcccaaa gtgtactcaa aaaatgtacc
     6181 aacctgtatg actgctgagt ccgcaagtac gacagaatgg ctagcaatac aagggctacc
     6241 aatggttctt agttggatta ttggtactaa tgaaaaaaaa gcacagatgg aactctataa
     6301 tgaaattgcg acagaatatg gtcatgatat atctaaaata gatcattgta tgacttatat
     6361 ttgttctgtt gatgatgatg cacaaaaggc gcaagatgtt tgtcgggagt ttctgaaaaa
     6421 ttggtatgac tcatatgtaa atgcgaccaa tatctttaat gatagcaatc aaactcgtgg
     6481 ttatgattat cataaaggtc aatggcgtga ttttgtttta caaggacata caaacaccaa
     6541 tcgacgtgtt gattatagca atggtattaa ccctgtaggc actcctgagc agtgtattga
     6601 aatcattcaa cgtgatattg atgcaacggg tattacaaac attacatgcg gatttgaagc
     6661 taatggaact gaagatgaaa taattgcttc catgcgacgc tttatgacac aagtcgctcc
     6721 tttcttaaaa gaacctaaat aaattactta tttgatacta gagataataa ggaacaagtt
     6781 atgaaatttg gattattttt tctaaacttt cagaaagatg gaataacatc tgaagaaacg
     6841 ttggataata tggtaaagac tgtcacgtta attgattcaa ctaaatatca ttttaatact
     6901 gcctttgtta atgaacatca cttttcaaaa aatggtattg ttggagcacc tattaccgca
     6961 gctggttttt tattagggtt aacaaataaa ttacatattg gttcattaaa tcaagtaatt
     7021 accacccatc accctgtacg tgtagcagaa gaagccagtt tattagatca aatgtcagag
     7081 ggacgcttca ttcttggttt tagtgactgc gaaagtgatt tcgaaatgga attttttaga
     7141 cgtcatatct catcaaggca acaacaattt gaagcatgct atgaaataat taatgacgca
     7201 ttaactacag gttattgtca tccccaaaac gacttttatg attttccaaa ggtttcaatt
     7261 aatccacact gttacagtga gaatggacct aagcaatatg tatccgctac atcaaaagaa
     7321 gtcgtcatgt gggcagcgaa aaaggcactg cctttaacat ttaagtggga ggataattta
     7381 gaaaccaaag aacgctatgc aattctatat aataaaacag cacaacaata tggtattgat
     7441 atttcggatg ttgatcatca attaactgta attgcgaact taaatgctga tagaagtacg
     7501 gctcaagaag aagtgagaga atacttaaaa gactatatca ctgaaactta ccctcaaatg
     7561 gacagagatg aaaaaattaa ctgcattatt gaagagaatg cagttgggtc tcatgatgac
     7621 tattatgaat cgacaaaatt agcagtggaa aaaacagggt ctaaaaatat tttattatcc
     7681 tttgaatcaa tgtccgatat taaagatgta aaagatatta ttgatatgtt gaaccaaaaa
     7741 atcgaaatga atttaccata ataaaattaa aggcaatttc tatattagat tgccttttta
     7801 aatttctgtt gatattaggt attactggag agggtatgac tgtccatact gaatataaaa
     7861 gaaatcaaat cattgctagt tcagaaattg atgatcttat ctttatgacg aaaccacaag
     7921 agtggtcatt tgaagagcaa aaagaaatac gggataaatt agttcgtgag gctttttatt
     7981 ttcactacaa tagaaatgaa gaatatagaa attattgtat caatcagcat gtgagtgata
     8041 atttacacac tattgatgaa atacccgtgt ttccaacatc tgtttttaaa tataagaaat
     8101 tacatactgt cacagccgag gacattgaaa attggtatac aagtagtgga actcgtggag
     8161 taaaaagtca tattgcacgt gatcgtctta gcattgaacg cttgcttggt tctgtcaact
     8221 tcggaatgaa atacgttgga gattggtttg agcatcaaat ggaattgata aatttaggac
     8281 cagatagatt caatacaaat aatatttggt ttaaatatgt catgagtttg gtcgagttac
     8341 tttatccgac tgaatttaca gttgataatg acaaaataga ttttgaaaaa acagtaaaac
     8401 atctatttag aattaagaat agtaaaaaag acatttgctt aattgggcca ccattttttg
     8461 tgtatctttt gtgccaatat atgaaagaaa acaatattga atttaaagga ggagatagag
     8521 tacatattat tactggtgga ggatggaaat ctaatcagaa tgactcttta gatcgtgctg
     8581 attttaatca attattaatg gatactttcc aactcgacaa gattaatcaa attagagata
     8641 cctttaatca agttgagctt aatacttgtt tttttgaaga tgaatttcaa agaaaacatg
     8701 ttccaccgtg ggtatatgct cgggctcttg atcctgaaac cttgaaaccc gtagcagatg
     8761 gtgagatcgg gttgttaagt tatatggacg cctcatcaac tgcttaccct gcttttattg
     8821 ttactgatga tatcggtatt gtaaaagaaa ttagagaacc agatccttac ccaggggtaa
     8881 ctgttgagat tgttcggcgc ttaaatacac gtgcgcaaaa aggatgcgcg ctctctatgg
     8941 ctaatgtcat acaaaagaat atcaaggatt aagttatgat tgttgatggt agagtttcaa
     9001 aaatagtatt agcgtcaata agaaataata tatataaggt atttattact gtaaattcac
     9061 caataaagtt catcgctgga caatttgtaa tggtcacgat taatgggaaa aaatgccctt
     9121 tttcaattgc gaattgcccg acaaaaaatt acgaaataga attgcatatt ggtagttcga
     9181 atagagactg ctcattggat attatcgaat attttgtcga tgctcttgtt gaggaagtcg
     9241 caattgagtt agatgctccc catggaaacg cttggttacg gtctgaaagt aataacccat
     9301 tgctattaat tgcgggaggt acaggtttat catatataaa tagcattcta acaaattgct
     9361 taaataggaa tatacctcaa gatatttatc tttactgggg agtaaaagac agttctcttt
     9421 tgtatgaaga tgaagagtta ctaaacttat cactaaacaa caaaaacttt cattatattc
     9481 ctgttattga agataaaagt gaagaatgga tagggagaaa aggcactgtt cttgatgctg
     9541 tcatggaaga ttttactgat cttacttatt ttgatattta tgtttgtgga cccttcatga
     9601 tggctaaaac agcaaaagaa aaattaattg aagagaaaaa agcaaagtca gaacagatgt
     9661 ttgccgatgc ttttgcatac gtataatact agtagcggcc gctgcag
//
