LOCUS       sequence-206256-        2299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), 
            CmR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, 
            Awan AR, Gilbert C, Stan GB, Ellis T
  TITLE     Burden-driven feedback control of gene expression.
  JOURNAL   Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635.
  PUBMED    29578536
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635."
COMMENT     Sequence Label: pLys-M1 backbone
FEATURES             Location/Qualifiers
     source          1..2299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(20..25,26..42,43..48))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      160..704
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     605..624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     821..838
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1230..1332
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1333..1992
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1399..1418)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctagtagata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaatta
       61 caacttatat cgtatggggc tgacttcagg tgctacattt gaagagataa attgcactga
      121 aatctagaaa tattttatct gattaataag atgatcttct tgagatcgtt ttggtctgcg
      181 cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct
      241 ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact
      301 tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat
      361 taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat
      421 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
      481 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
      541 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg
      601 ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
      661 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg
      721 ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc
      781 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
      841 agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct
      901 gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacac
      961 tccgctagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcagtagctg
     1021 aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa caccatcata
     1081 cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
     1141 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
     1201 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     1261 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1321 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     1381 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     1441 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     1501 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     1561 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     1621 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     1681 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1741 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     1801 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     1861 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     1921 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     1981 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct
     2041 gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg acccggtcgt
     2101 cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta ccggtttatt
     2161 gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt ttgctcaggc
     2221 tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc agagcctgat
     2281 aaactctaga agcggccgc
//
