LOCUS       sequence-206258-        8694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  V5 and His-tagged human ANGPTL8 in a lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Chi X, Britt EC, Shows HW, Hjelmaas AJ, Shetty SK, Cushing EM, Li W,
            Dou A, Zhang R, Davies BSJ
  TITLE     ANGPTL8 promotes the ability of ANGPTL3 to bind and inhibit 
            lipoprotein lipase.
  JOURNAL   Mol Metab. 2017 Oct;6(10):1137-1149. doi: 
            10.1016/j.molmet.2017.06.014. Epub 2017 Jun 29.
  PUBMED    29031715
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molmet.2017.06.014"; journalName: "Mol Metab"; date: 
            "2017-10"; volume: "6"; issue: "10"; pages: "1137-1149"
COMMENT     Sequence Label: pEB31 (hANGPTL8-V5-His lenti)
FEATURES             Location/Qualifiers
     source          1..8694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        29..133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(134..202,203..994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(352..371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1165..1753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1654..1673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1907..1924
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2041..2062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2077..2082,2083..2100,2101..2107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2115..2131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2120..2142
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2139..2155
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2139..2155
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2174..2194
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2176..2194
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2222..2448
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2449..2629
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2676..2801
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3294..3527
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3712..3756
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4023..4140
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4162..4465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4466..4669
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4620..4640
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4666..4690
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5346..5387
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5397..5414
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    5615..6077
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5671..5688)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5898..5917
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6110..6709
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(6110..6129)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6606..6626
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6757..7345
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6810..6830)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7228..7239)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7431..7664
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7736..7857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7773..7792)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7827..7846
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7897..8032
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8052..8071)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8053..8071)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8081..8098)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8081..8097)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8090..8112)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8239..8694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8326..8345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8536..8557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
       61 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      121 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      241 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      301 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      361 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      421 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      541 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      661 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
      721 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
      781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      841 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      961 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1021 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1081 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1801 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1921 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1981 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2041 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2101 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2161 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     2221 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     2281 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     2341 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     2401 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     2461 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2521 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2581 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     2641 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     2701 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2761 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     2821 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     2881 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     2941 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     3001 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     3061 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     3121 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     3181 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     3241 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     3301 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     3361 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     3421 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     3481 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     3541 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     3601 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     3661 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     3721 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     3781 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     3841 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     3901 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     3961 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     4021 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     4081 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     4141 ccgataagct tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     4201 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4261 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4321 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4381 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4441 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     4501 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     4561 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     4621 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     4681 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact
     4741 ctagaactag tatgccagtg cctgctctgt gcctgctctg ggccctggca atggtgaccc
     4801 ggcctgcctc agcggccccc atgggcggcc cagaactggc acagcatgag gagctgaccc
     4861 tgctcttcca tgggaccctg cagctgggcc aggccctcaa cggtgtgtac aggaccacgg
     4921 agggacggct gacaaaggcc aggaacagcc tgggtctcta tggccgcaca atagaactcc
     4981 tggggcagga ggtcagccgg ggccgggatg cagcccagga acttcgggca agcctgttgg
     5041 agactcagat ggaggaggat attctgcagc tgcaggcaga ggccacagct gaggtgctgg
     5101 gggaggtggc ccaggcacag aaggtgctac gggacagcgt gcagcggcta gaagtccagc
     5161 tgaggagcgc ctggctgggc cctgcctacc gagaatttga ggtcttaaag gctcacgctg
     5221 acaagcagag ccacatccta tgggccctca caggccacgt gcagcggcag aggcgggaga
     5281 tggtggcaca gcagcatcgg ctgcgacaga tccaggagag actccacaca gcggctctcc
     5341 cagccggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc
     5401 atcaccatca ccattgaaga cccgttcgaa ttcgaagctt gagctcgaga tctgagtccg
     5461 gtagcgctag ttcgaaggat ccgcatgcat ctagggcggc caattccgcc cctctccccc
     5521 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     5581 gattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     5641 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     5701 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     5761 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     5821 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     5881 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     5941 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     6001 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     6061 gaaaaacacg atgataagct tgccacaacc cacaaggaga cgaccttcca tgaccgagta
     6121 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     6181 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     6241 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6301 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6361 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6421 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6481 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6541 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6601 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgagtg
     6661 cccgaaggac cgcgcgacct ggtgcatgac ccgcaagccc ggtgcctgat aataggcggc
     6721 cgctcgagac ctagaaaaac aatcgatacc gtcgacaatc aacctctgga ttacaaaatt
     6781 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6841 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6901 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6961 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7021 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7081 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7141 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7201 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7261 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7321 atctcccttt gggccgcctc cccgcctgaa tacgagctcg gtacctttaa gaccaatgac
     7381 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7441 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     7501 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7561 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7621 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7681 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7741 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7801 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7861 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7921 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7981 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     8041 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     8101 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     8161 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     8221 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     8281 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     8341 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     8401 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     8461 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     8521 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     8581 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     8641 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attt
//
