LOCUS       sequence-206264-       15198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in 
            vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15198)
  AUTHORS   Akera T, Chmatal L, Trimm E, Yang K, Aonbangkhen C, Chenoweth DM, 
            Janke C, Schultz RM, Lampson MA
  TITLE     Spindle asymmetry drives non-Mendelian chromosome segregation.
  JOURNAL   Science. 2017 Nov 3;358(6363):668-672. doi: 10.1126/science.aan0092.
  PUBMED    29097549
REFERENCE   2  (bases 1 to 15198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2017-11-3- 3"; volume: "358"; issue: 
            "6363"; pages: "668-672"
COMMENT     Sequence Label: 6x Halo-EGFP- 2x PACT
FEATURES             Location/Qualifiers
     source          1..15198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             62..952
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             980..1867
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     regulatory      1898..1907
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1904..2794
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     regulatory      2801..2810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2825..3715
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             3743..4630
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     regulatory      4661..4670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4667..5557
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     regulatory      5564..5573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5570..5572,5573..5575,5576..6286)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5570..5572,5573..5575,5576..6286)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5615..5636)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5876..5895)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6223..6244
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7392..7965
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7415..7967
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7559..7576)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7786..7805
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7967..8674
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8112..8130)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8380..8399
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8693..9166
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             9167..9196
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(9303..9322)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9368..9423
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9422..9441)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    9787..9820
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    9833..9954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9870..9889)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9924..9943
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9973..9993)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9973..9991)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10012..10028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10012..10028)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10025..10047)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10036..10052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10060..10066,10067..10084,10085..10090))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10105..10126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10243..10260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10414..11002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10494..10513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11173..11964,11965..12033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11796..11815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12034..12138)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12211..12259
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    12471..12504
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             12509..13108
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12509..12528)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13005..13025
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(13244..13261)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13250..13361
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        13506..13809
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          14089..15105
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     15029..15051
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     15113..15132
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 taatacgact cactataggg agacccaagc tctagcgcta ccggtcgcca cccgcgccac
       61 aatggcagag atcggtactg gcttcccatt cgatccccat tacgtggaag tcctgggcga
      121 acgcatgcac tatgtcgatg ttggtccgcg cgacggcacc cctgtgctgt tcctgcatgg
      181 taacccgacc tcctcctatg tgtggcgcaa cattatcccg cacgttgcac cgacccatcg
      241 ctgtattgct ccagatctga tcggtatggg caagtccgac aagccagacc tgggttactt
      301 ctttgacgat cacgtccgct ttatggatgc ctttatcgag gccctgggtc tggaagaagt
      361 cgtcctggtc attcatgact ggggctccgc tctgggtttt cactgggcca aacgcaatcc
      421 agaacgcgtc aaaggtatcg catttatgga attcattcgc cctatcccga cctgggacga
      481 atggccagag tttgcccgcg aaaccttcca ggcctttcgc accaccgacg tcggccgcaa
      541 actgatcatt gatcagaatg tttttattga gggtacgctg ccgatgggtg tcgtccgccc
      601 gctgactgag gtcgagatgg atcattatcg cgagccgttt ctgaatcctg ttgatcgcga
      661 gccactgtgg cgctttccaa acgaactgcc aatcgccggt gaaccagcga acattgtcgc
      721 gctggtcgaa gagtacatgg attggctgca ccagtcccct gtcccgaaac tgctgttctg
      781 gggcacccca ggcgttctga ttccaccggc cgaagccgct cgcctggcca agagcctgcc
      841 taactgcaaa gctgtggaca ttggcccggg tctgaatctg ctgcaagagg acaatccgga
      901 cctgattggc agcgagattg cgcgctggct gtcgacgctc gagatctccg gcgcttcgaa
      961 ttctgcagtc gacggtaccg cagaaattgg tactggcttt ccatttgacc cccactatgt
     1021 ggaggtcctg ggcgagcgca tgcattacgt cgacgttggt ccgcgcgatg gcacccctgt
     1081 gctgtttctg cacggtaatc cgacctcctc ctacgtgtgg cgcaatatca ttccgcatgt
     1141 tgcaccgacc caccgctgca tcgctccaga cctgattggt atgggcaaat ccgataaacc
     1201 agatctgggt tattttttcg atgaccatgt ccgcttcatg gacgccttca ttgaagccct
     1261 gggtctggag gaggtcgtcc tggtcatcca cgattggggc tccgctctgg gtttccattg
     1321 ggccaagcgc aacccagagc gcgtcaaggg tattgcattc atggagttta tccgccctat
     1381 tccgacctgg gacgagtggc cagaattcgc ccgcgagacc tttcaggcct tccgcaccac
     1441 cgatgtcggc cgcaagctga ttatcgacca gaacgttttc atcgaaggta cgctgccgat
     1501 gggtgtcgtc cgcccgctga ctgaagtcga aatggaccac taccgcgaac cgttcctgaa
     1561 ccctgttgac cgcgaaccac tgtggcgctt cccaaatgag ctgccaattg ccggtgagcc
     1621 agcgaatatc gtcgcgctgg tcgaggaata tatggactgg ctgcatcagt cccctgtccc
     1681 gaagctgctg ttttggggca ccccaggcgt tctgatccca ccggccgagg ccgctcgcct
     1741 ggccaaaagc ctgcctaatt gcaaggctgt ggatatcggc ccgggtctga acctgctgca
     1801 agaagataac ccggatctga tcggcagcga aatcgcgcgc tggctgtcga cgctcgaaat
     1861 ttccggcagt gctggtagtg ctggtgaatt gatgcgcgcc accatggcag aaatcggtac
     1921 tggctttcca ttcgaccccc attatgtgga agtcctgggc gagcgcatgc actacgtcga
     1981 tgttggtccg cgcgatggca cccctgtgct gttcctgcac ggtaacccga cctcctccta
     2041 cgtgtggcgc aacatcatcc cgcatgttgc accgacccat cgctgcattg ctccagacct
     2101 gatcggtatg ggcaaatccg acaaaccaga cctgggttat ttcttcgacg accacgtccg
     2161 cttcatggat gccttcatcg aagccctggg tctggaagag gtcgtcctgg tcattcacga
     2221 ctggggctcc gctctgggtt tccactgggc caagcgcaat ccagagcgcg tcaaaggtat
     2281 tgcatttatg gagttcatcc gccctatccc gacctgggac gaatggccag aatttgcccg
     2341 cgagaccttc caggccttcc gcaccaccga cgtcggccgc aagctgatca tcgatcagaa
     2401 cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg aagtcgagat
     2461 ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt ggcgcttccc
     2521 aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg aagaatacat
     2581 ggactggctg caccagtccc ctgtcccgaa gctgctgttc tggggcaccc caggcgttct
     2641 gatcccaccg gccgaagccg ctcgcctggc caaaagcctg cctaactgca aggctgtgga
     2701 catcggcccg ggtctgaatc tgctgcaaga agacaacccg gacctgatcg gcagcgagat
     2761 cgcgcgctgg ctgtcgacgc tcgagatttc cggttacgta gccaccatgg tggggcgcgc
     2821 cacaatggca gagatcggta ctggcttccc attcgatccc cattacgtgg aagtcctggg
     2881 cgaacgcatg cactatgtcg atgttggtcc gcgcgacggc acccctgtgc tgttcctgca
     2941 tggtaacccg acctcctcct atgtgtggcg caacattatc ccgcacgttg caccgaccca
     3001 tcgctgtatt gctccagatc tgatcggtat gggcaagtcc gacaagccag acctgggtta
     3061 cttctttgac gatcacgtcc gctttatgga tgcctttatc gaggccctgg gtctggaaga
     3121 agtcgtcctg gtcattcatg actggggctc cgctctgggt tttcactggg ccaaacgcaa
     3181 tccagaacgc gtcaaaggta tcgcatttat ggaattcatt cgccctatcc cgacctggga
     3241 cgaatggcca gagtttgccc gcgaaacctt ccaggccttt cgcaccaccg acgtcggccg
     3301 caaactgatc attgatcaga atgtttttat tgagggtacg ctgccgatgg gtgtcgtccg
     3361 cccgctgact gaggtcgaga tggatcatta tcgcgagccg tttctgaatc ctgttgatcg
     3421 cgagccactg tggcgctttc caaacgaact gccaatcgcc ggtgaaccag cgaacattgt
     3481 cgcgctggtc gaagagtaca tggattggct gcaccagtcc cctgtcccga aactgctgtt
     3541 ctggggcacc ccaggcgttc tgattccacc ggccgaagcc gctcgcctgg ccaagagcct
     3601 gcctaactgc aaagctgtgg acattggccc gggtctgaat ctgctgcaag aggacaatcc
     3661 ggacctgatt ggcagcgaga ttgcgcgctg gctgtcgacg ctcgagatct ccggcgcttc
     3721 gaattctgca gtcgacggta ccgcagaaat tggtactggc tttccatttg acccccacta
     3781 tgtggaggtc ctgggcgagc gcatgcatta cgtcgacgtt ggtccgcgcg atggcacccc
     3841 tgtgctgttt ctgcacggta atccgacctc ctcctacgtg tggcgcaata tcattccgca
     3901 tgttgcaccg acccaccgct gcatcgctcc agacctgatt ggtatgggca aatccgataa
     3961 accagatctg ggttattttt tcgatgacca tgtccgcttc atggacgcct tcattgaagc
     4021 cctgggtctg gaggaggtcg tcctggtcat ccacgattgg ggctccgctc tgggtttcca
     4081 ttgggccaag cgcaacccag agcgcgtcaa gggtattgca ttcatggagt ttatccgccc
     4141 tattccgacc tgggacgagt ggccagaatt cgcccgcgag acctttcagg ccttccgcac
     4201 caccgatgtc ggccgcaagc tgattatcga ccagaacgtt ttcatcgaag gtacgctgcc
     4261 gatgggtgtc gtccgcccgc tgactgaagt cgaaatggac cactaccgcg aaccgttcct
     4321 gaaccctgtt gaccgcgaac cactgtggcg cttcccaaat gagctgccaa ttgccggtga
     4381 gccagcgaat atcgtcgcgc tggtcgagga atatatggac tggctgcatc agtcccctgt
     4441 cccgaagctg ctgttttggg gcaccccagg cgttctgatc ccaccggccg aggccgctcg
     4501 cctggccaaa agcctgccta attgcaaggc tgtggatatc ggcccgggtc tgaacctgct
     4561 gcaagaagat aacccggatc tgatcggcag cgaaatcgcg cgctggctgt cgacgctcga
     4621 aatttccggc agtgctggta gtgctggtga attgatgcgc gccaccatgg cagaaatcgg
     4681 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     4741 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     4801 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     4861 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     4921 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     4981 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     5041 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     5101 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     5161 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     5221 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     5281 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     5341 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     5401 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     5461 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     5521 gatcgcgcgc tggctgtcga cgctcgagat ttccggttac gtagccacca tggtgagcaa
     5581 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5641 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     5701 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5761 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     5821 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     5881 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5941 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     6001 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     6061 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     6121 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     6181 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     6241 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggtg gtgaatccgg
     6301 tgatgcatcg tatcgattgc tagccaacat tgaagccatc attgcctctg aaaaagaagt
     6361 atggaacaga gaaaaattga ctctccagaa atctttgaaa agggcagagg ctgaagtata
     6421 caaactgaaa gctgaactaa gaaatgactc tttacttcaa actctgagcc ctgattctga
     6481 acatgtcact ttaaagagaa tttatggtaa atacttgagg gcagaaagtt ttcgaaaggc
     6541 tctcatttac cagaagaaat acctgctgct gttactgggt gggttccagg aatgtgaaga
     6601 tgccaccttg gccctgcttg cccggatggg ggggcagcca gctttcacgg atctagaggt
     6661 gatcaccaat cgcccaaagg gcttcaccag gtttcggtcg gccgtcagag tatccattgc
     6721 aatttccaga atgaaatttt tggttcgacg gtggcatcga gtcacaggtt ctgtttccat
     6781 caatattaac agagatggct ttggactgaa tcaaggtgca ctgtacaagg gtggtgaatc
     6841 cggtgatgca tcgtatcgat tgctagccaa cattgaagcc atcattgcct ctgaaaaaga
     6901 agtatggaac agagaaaaat tgactctcca gaaatctttg aaaagggcag aggctgaagt
     6961 atacaaactg aaagctgaac taagaaatga ctctttactt caaactctga gccctgattc
     7021 tgaacatgtc actttaaaga gaatttatgg taaatacttg agggcagaaa gttttcgaaa
     7081 ggctctcatt taccagaaga aatacctgct gctgttactg ggtgggttcc aggaatgtga
     7141 agatgccacc ttggccctgc ttgcccggat gggggggcag ccagctttca cggatctaga
     7201 ggtgatcacc aatcgcccaa agggcttcac caggtttcgg tcggccgtca gagtatccat
     7261 tgcaatttcc agaatgaaat ttttggttcg acggtggcat cgagtcacag gttctgtttc
     7321 catcaatatt aacagagatg gctttggact gaatcaaggt gcatccggat gacgataagc
     7381 ttaaggatcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     7441 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     7501 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     7561 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     7621 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     7681 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     7741 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     7801 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     7861 gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     7921 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg tgagcaaggg
     7981 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     8041 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     8101 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     8161 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     8221 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     8281 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     8341 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     8401 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     8461 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     8521 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     8581 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     8641 ccactccacc ggcggcatgg acgagctgta taaggccggc ctggagtcta gaatcagtct
     8701 gattgcggcg ttagcggtag atcgcgttat cggcatggaa aacgccatgc cgtggaacct
     8761 gcctgccgat ctcgcctggt ttaaacgcaa caccttaaat aaacccgtga ttatgggccg
     8821 ccatacctgg gaatcaatcg gtcgtccgtt gccaggacgc aaaaatatta tcctcagcag
     8881 tcaaccgggt acggacgatc gcgtaacgtg ggtgaagtcg gtggatgaag ccatcgcggc
     8941 gtgtggtgac gtaccagaaa tcatggtgat tggcggcggt cgcgtttatg aacagttctt
     9001 gccaaaagcg caaaaactgt atctgacgca tatcgacgca gaagtggaag gcgacaccca
     9061 tttcccggat tacgagccgg atgactggga atcggtattc agcgaattcc acgatgctga
     9121 tgcgcagaac tctcacagct attgctttga gattctggag cggcgggaac aaaaactcat
     9181 ctcagaagag gatctttaat taagcgctcg aggcggccgc aattcactcc tcaggtgcag
     9241 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     9301 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     9361 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     9421 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     9481 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     9541 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     9601 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     9661 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     9721 atagctgtcc ctcttctctt atggagatcc ctcgacctgc accgtcgagc tagcatatgg
     9781 atccatataa cttcgtataa tgtatgctat acgaagttat tacgataccg tcaacttgtt
     9841 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9901 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaggtacc
     9961 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
    10021 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10081 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    10141 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    10201 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    10261 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10321 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10381 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10441 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10501 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10561 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10621 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10681 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10741 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10801 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    10861 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10921 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10981 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    11041 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    11101 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    11161 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    11221 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    11281 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11341 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11401 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11461 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    11521 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11581 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    11641 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    11701 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11761 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11821 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11881 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11941 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    12001 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    12061 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    12121 acaaataggg gttccgcgca catttccccg aaaagtgcca agcttaataa acacggaagg
    12181 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
    12241 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
    12301 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
    12361 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
    12421 atagccactg gccccgtggg ttagggacgg ggtcccccat tcgagaataa ataacttcgt
    12481 ataaagtatc ctatacgaag ttatcaccat gaccgagtac aagcccacgg tgcgcctcgc
    12541 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
    12601 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
    12661 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
    12721 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
    12781 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
    12841 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
    12901 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
    12961 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
    13021 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    13081 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
    13141 aaggagcgca cgaccccatg gctccgaccg aagccacccg gggcggcccc gccgaccccg
    13201 cacccgcccc cgaggcccac cgactctaga gctcgctgat cagcctcgac tgtgccttct
    13261 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
    13321 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
    13381 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
    13441 agcactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
    13501 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
    13561 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
    13621 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
    13681 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    13741 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    13801 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
    13861 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
    13921 gggggggggg ggcgcgcgcc gggggggggg gggggggggg gggggggggg gggggggggg
    13981 cggggggggg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
    14041 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
    14101 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
    14161 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
    14221 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
    14281 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
    14341 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
    14401 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
    14461 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
    14521 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
    14581 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
    14641 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
    14701 gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc
    14761 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
    14821 ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
    14881 cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
    14941 tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc
    15001 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
    15061 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
    15121 ctggttattg tgctgtctca tcattttggc aaagaattgc tcgaccaccg gcgcgctgca
    15181 gtacggcgcg atcgaaat
//
