LOCUS       sequence-206272-       13804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13804)
  AUTHORS   Scholl FA, Dumesic PA, Khavari PA
  TITLE     Mek1 alters epidermal growth and differentiation
  JOURNAL   Cancer Res. 2004 Sep 1;64(17):6035-40
  PUBMED    15342384
REFERENCE   2  (bases 1 to 13804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2004-09-1"; volume: "64(17)"; pages: "6035-4"
COMMENT     Sequence Label: LZRS-Rfa
FEATURES             Location/Qualifiers
     source          1..13804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             494..963
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1028..1385
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1450..1866
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1796..1818
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1834..1850
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1876..2250
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2188..2204
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    2280..2404
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2429..2434,2435..2452,2453..2459)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2513..3172
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2579..2598)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3775..4080
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3959..3978
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4121..4245)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             4558..5027
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5157..5174)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5328..5916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5408..5427)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6039..6173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6063..6082)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6117..6136
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6403..7002)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6486..6506)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6983..7002
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7085..7584)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7133..7152)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7526..7549
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             8013..9938
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      10240..12029
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(12557..13348,13349..13417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13180..13199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13418..13522)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcctaataca tcccaaactc aaatatataa agcatttgac ttgttctatg ccctaggggg
       61 cggggggaag ctaagccagc tttttttaac atttaaaatg ttaattccat tttaaatgca
      121 cagatgtttt tatttcataa gggtttcaat gtgcatgaat gctgcaatat tcctgttacc
      181 aaagctagta taaataaaaa tagataaacg tggaaattac ttagagtttc tgtcattaac
      241 gtttccttcc tcagttgaca acataaatgc gctgctgaga agccagtttg catctgtcag
      301 gatcaatttc ccattatgcc agtcatatta attactagtc aattagttga tttttatttt
      361 tgacatatac atgtgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
      421 ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc aaggtcagga
      481 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
      541 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
      601 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct
      661 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
      721 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
      781 ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgattgac
      841 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
      901 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
      961 tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc
     1021 gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg tctatgactg
     1081 attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg cggacccgtg
     1141 gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc agggacttcg
     1201 ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg actctttggt
     1261 gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct aaaacagttc
     1321 ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc gcgtcttgtc
     1381 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
     1441 atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat
     1501 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc
     1561 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga
     1621 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
     1681 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
     1741 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
     1801 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
     1861 ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct tgtaaacttc
     1921 cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca cttacaggct
     1981 ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca agaacaactg
     2041 gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt ccgccgacac
     2101 cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct gaccaccccc
     2161 accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt gaaggctgcc
     2221 gaccccgggg gtggaccatc ctctagactg ccggatccca gtgtggtggt acgggaatca
     2281 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2341 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2401 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
     2461 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
     2521 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     2581 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     2641 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     2701 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
     2761 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     2821 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     2881 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     2941 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     3001 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     3061 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     3121 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg
     3181 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     3241 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     3301 cgtattacag ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     3361 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     3421 gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt
     3481 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
     3541 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
     3601 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
     3661 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
     3721 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag
     3781 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
     3841 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
     3901 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
     3961 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat
     4021 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
     4081 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg
     4141 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
     4201 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatat tccagctgag
     4261 cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc
     4321 atgtctgccc gtatttcgcg taaggaaatc cattatgtac tattttcgag cggccgccag
     4381 cacagtggtc gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     4441 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     4501 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     4561 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     4621 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     4681 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     4741 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     4801 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     4861 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     4921 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     4981 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta
     5041 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa
     5101 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5161 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5221 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5281 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5341 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5401 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5461 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5521 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5581 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5641 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5701 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5761 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5821 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5881 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5941 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6001 aaaaggatct tcacctagat ccttttgcgg ccgaattaga tccagacatg ataagataca
     6061 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     6121 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     6181 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     6241 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagagtcg gtgggcctcg
     6301 ggggcgggtg cggggtcggc ggggccgccc cgggtggctt cggtcggagc catggggtcg
     6361 tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg
     6421 ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc
     6481 gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc
     6541 ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg
     6601 gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg
     6661 cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat
     6721 gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac
     6781 cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt
     6841 gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccgggt cgacggtgtg
     6901 gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc gggggacgtc
     6961 gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggaaggtc gtctccttgt
     7021 gaggggtcag gggcgtgggt caggggatgg tggcggcacc ggtcgtggcg gccgacctgc
     7081 aggtcgaaag gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa
     7141 gcgtgcagaa tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc
     7201 ccctgagccc gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc
     7261 aaagctgcta ttggccgctg ccccaaaggc ctacccgctt ccattgctca gcggtgctgt
     7321 ccatctgcac gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga
     7381 gctgcggggc gggggggaac ttcctgacta ggggaggagt agaaggtggc gcgaaggggc
     7441 caccaaagaa cggagccggt tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga
     7501 ggccacttgt gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc
     7561 ttgggaaaag cgcctcccct acccggtaga attaattctc atgtttgaca gcttatcatc
     7621 gataagctga tcctcacagg ccgcacccag cttttcttcc gttgccccag tagcatctct
     7681 gtctggtgac cttgaagagg aagaggaggg gtcccgagaa tccccatccc taccgtccag
     7741 caaaaagggg gacgaggaat ttgaggcctg gcttgaggct caggacgcaa atcttgagga
     7801 tgttcagcgg gagttttccg ggctgcgagt aattggtgat gaggacgagg atggttcgga
     7861 ggatggggaa ttttcagacc tggatctgtc tgacagcgac catgaagggg atgagggtgg
     7921 gggggctgtt ggagggggca ggagtctgca ctccctgtat tcactgagcg tcgtctaata
     7981 aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac
     8041 ctggaaatgg cctaggagag aagggagaca catctggacc agaaggctcc ggcggcagtg
     8101 gacctcaaag aagagggggt gataaccatg gacgaggacg gggaagagga cgaggacgag
     8161 gaggcggaag accaggagcc ccgggcggct caggatcagg gccaagacat agagatggtg
     8221 tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa agggacccac ggtggaacag
     8281 gagcaggagc aggagcggga ggggcaggag caggaggggc aggagcagga ggaggggcag
     8341 gagcaggagg aggggcagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg
     8401 caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag
     8461 caggaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca ggaggggcag
     8521 gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggagggg
     8581 caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg gcaggagggg
     8641 caggaggggc aggagcagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg
     8701 caggagcagg aggggcagga gcaggagggg caggagcagg aggggcagga gcaggagggg
     8761 caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag
     8821 gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag
     8881 gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag
     8941 gggcaggagc aggaggaggg gcaggagcag gaggggcagg agcaggaggt ggaggccggg
     9001 gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg aggccggggt cgaggaggta
     9061 gtggaggccg ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt gaaagagcca
     9121 gggggagagg tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt cagtcatcat
     9181 catccgggtc tccaccgcgc aggccccctc caggtagaag gccatttttc caccctgtag
     9241 gggaagccga ttattttgaa taccaccaag aaggtggccc agatggtgag cctgacgtgc
     9301 ccccgggagc gatagagcag ggccccgcag atgacccagg agaaggccca agcactggac
     9361 cccggggtca gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga aagcatcgtg
     9421 gtcaaggagg ttccaacccg aaatttgaga acattgcaga aggtttaaga gctctcctgg
     9481 ctaggagtca cgtagaaagg actaccgacg aaggaacttg ggtcgccggt gtgttcgtat
     9541 atggaggtag taagacctcc ctttacaacc taaggcgagg aactgccctt gctattccac
     9601 aatgtcgtct tacaccattg agtcgtctcc cctttggaat ggcccctgga cccggcccac
     9661 aacctggccc gctaagggag tccattgtct gttatttcat ggtcttttta caaactcata
     9721 tatttgctga ggttttgaag gatgcgatta aggaccttgt tatgacaaag cccgctccta
     9781 cctgcaatat cagggtgact gtgtgcagct ttgacgatgg agtagatttg cctccctggt
     9841 ttccacctat ggtggaaggg gctgccgcgg agggtgatga cggagatgac ggagatgaag
     9901 gaggtgatgg agatgagggt gaggaagggc aggagtgatg taacttgtta ggagacgccc
     9961 tcaatcgtat taaaagccgt gtattccccc gcactaaaga ataaatcccc agtagacatc
    10021 atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca ccattttcgt cctcccaaca
    10081 tggggcaatt gggcataccc atgttgtcac gtcactcagc tccgcgctca acaccttctc
    10141 gcgttggaaa acattagcga catttacctg gtgagcaatc agacatgcga cggctttagc
    10201 ctggcctcct taaattcacc taagaatggg agcaaccagc aggaaaagga caagcagcga
    10261 aaattcacgc ccccttggga ggtggcggca tatgcaaagg atagcactcc cactctacta
    10321 ctgggtatca tatgctgact gtatatgcat gaggatagca tatgctaccc ggatacagat
    10381 taggatagca tatactaccc agatatagat taggatagca tatgctaccc agatatagat
    10441 taggatagcc tatgctaccc agatataaat taggatagca tatactaccc agatatagat
    10501 taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat
    10561 taggatagca tatgctaccc agatatagat taggatagca tatgctatcc agatatttgg
    10621 gtagtatatg ctacccagat ataaattagg atagcatata ctaccctaat ctctattagg
    10681 atagcatatg ctacccggat acagattagg atagcatata ctacccagat atagattagg
    10741 atagcatatg ctacccagat atagattagg atagcctatg ctacccagat ataaattagg
    10801 atagcatata ctacccagat atagattagg atagcatatg ctacccagat atagattagg
    10861 atagcctatg ctacccagat atagattagg atagcatatg ctatccagat atttgggtag
    10921 tatatgctac ccatggcaac attagcccac cgtgctctca gcgacctcgt gaatatgagg
    10981 accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc tccagatcgc
    11041 agcaatcgcg cccctatctt ggcccgccca cctacttatg caggtattcc ccggggtgcc
    11101 attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta caatcagcca
    11161 agttattaca cccttatttt acagtccaaa accgcagggc ggcgtgtggg ggctgacgcg
    11221 tgcccccact ccacaatttc aaaaaaaaga gtggccactt gtctttgttt atgggcccca
    11281 ttggcgtgga gccccgttta attttcgggg gtgttagaga caaccagtgg agtccgctgc
    11341 tgtcggcgtc cactctcttt ccccttgtta caaatagagt gtaacaacat ggttcacctg
    11401 tcttggtccc tgcctgggac acatcttaat aaccccagta tcatattgca ctaggattat
    11461 gtgttgccca tagccataaa ttcgtgtgag atggacatcc agtctttacg gcttgtcccc
    11521 accccatgga tttctattgt taaagatatt cagaatgttt cattcctaca ctagtattta
    11581 ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc acaatgccac cactgaaccc
    11641 cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt gttttcgagc acctcacata
    11701 caccttactg ttcacaactc agcagttatt ctattagcta aacgaaggag aatgaagaag
    11761 caggcgaaga ttcaggagag ttcactgccc gctccttgat cttcagccac tgcccttgtg
    11821 actaaaatgg ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa ccgtgacagc
    11881 tcatggggtg ggagatatcg ctgttcctta ggaccctttt actaacccta attcgatagc
    11941 atatgcttcc cgttgggtaa catatgctat tgaattaggg ttagtctgga tagtatatac
    12001 tactacccgg gaagcatatg ctacccgttt agggttaaca agggggcctt ataaacacta
    12061 ttgctaatgc cctcttgagg gtccgcttat cggtagctac acaggcccct ctgattgacg
    12121 ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg tacatgtccc ccagcattgg
    12181 tgtaagagct tcagccaaga gttacacata aaggcaatgt tgtgttgcag tccacagact
    12241 gcaaagtctg ctccaggatg aaagccactc agtgttggca aatgtgcaca tccatttata
    12301 aggatgtcaa ctacagtcag agaacccctt tgtgtttggt ccccccccgt gtcacatgtg
    12361 gaacagggcc cagttggcaa gttgtaccaa ccaactgaag ggattacatg cactgccccg
    12421 cgaagaaggg gcagagatta catgcactgc cccgcgaaga aggggcagag atgtcgtagt
    12481 caggtttagt tcgtccgggg cggggatctg gccgcaaatc aatctaaagt atatatgagt
    12541 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    12601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    12661 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    12721 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    12781 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    12841 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    12901 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    12961 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    13021 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    13081 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    13141 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
    13201 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    13261 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    13321 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    13381 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    13441 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    13501 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccaccagct ttgctcttag
    13561 gagtttccta atacatccca aactcaaata tataaagcat ttgacttgtt ctatgcccta
    13621 gggggcgggg ggaagctaag ccagcttttt ttaacattta aaatgttaat tccattttaa
    13681 atgcacagat gtttttattt cataagggtt tcaatgtgca tgaatgctgc aatattcctg
    13741 ttaccaaagc tagtataaat aaaaatagat aaacgtggaa attacttaga gtttctgtca
    13801 ttaa
//
