LOCUS       sequence-206291-        3558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-248
FEATURES             Location/Qualifiers
     source          1..3558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..44
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1469..1487)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1555..1659
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1881..2672
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2843..3431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3332..3351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
       61 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtggtctca
      121 aatggatcaa gagatagaga ttccttcctt cttccttgct ccaatctctc tagatatcat
      181 gaaagatccg gtgatagttt ccaccggaat aacctacgac cgagaaagca tcgagaagtg
      241 gctcttttcc ggtaagaaaa actcatgtcc agtcaccaaa caagtcataa ccgaaactga
      301 tcttacccca aaccacactc ttcgccgttt gattcaatct tggtgtaccc tcaacgcatc
      361 atatggtata gagaggatcc caactccaaa acctccgatc tgtaaatcgg agatcgaaaa
      421 gcttatcaaa gagtcgtcat cttcgcatct aaaccaagtc aaatgcctca aacgacttcg
      481 tcaaatagta tctgagaata caaccaacaa acggtgctta gaagccgcag aagttccgga
      541 gtttttggca aatatcgtaa gcaactctgt agatacgtat aactctcctt cttcttcact
      601 ttcttcttcg aatctcaacg acatgtgtca gtcaaacatg ttggagaatc gatttgattc
      661 ttcaagaagc ttgatggacg aagccctaag cgtactctac catcttgaca catcggagac
      721 agccctcaag agtcttttaa acaacaagaa aggaacaaat cttgtgaaaa cgttgacgaa
      781 gattatgcaa cgtgggatct acgagtcaag agcctatgcg gctttgcttc tcaagaagct
      841 cctggaagtt gcggatccaa tgcagatcat attgttggaa cgagaacttt tcggtgaggt
      901 gattcaaatc ttgcacgacc agatctctca caaggcgaca agatcagcaa tgcaaatctt
      961 ggtgatcaca tgtccatggg gaaggaatag acacaaggca gtggaaggtg gaacaatctc
     1021 gatgataatc gagcttttaa tggacgatac tttctcatca gagagaagga attcagagat
     1081 ggcgatggtg gttcttgata tgttatgtca gtgcgcagaa ggaagagctg agttcttgaa
     1141 tcacggtgcc gctattgcgg ttgtgtctaa gaagatattg agggtatcac aaataactag
     1201 tgaaagggca gttagggttt tgctttcggt tgggaggttc tgcgcgacac cctctttgtt
     1261 gcaggagatg ttgcaattgg gagttgtagc aaagctgtgt ctggtacttc aagttagctg
     1321 tggtaacaag actaaagaaa aggctaaaga attgcttaag cttcacgcta gggtttggag
     1381 ggaatcacct tgtgtcccaa gaaatttgta tgattcgtat cctgcttgag ctttgagacc
     1441 acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat
     1501 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1561 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1621 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc
     1681 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     1741 gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     1801 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     1861 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
     1921 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     1981 acggctccgc agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg
     2041 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2101 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2161 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2221 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2281 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2341 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2401 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2461 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2521 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2581 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtaa
     2641 aaggcgagat caccaaggta gtcggcaaat aactgtcaga ccaagtttac tcatatatac
     2701 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2761 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2821 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2881 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2941 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3001 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3061 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3121 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3181 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3241 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3301 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3361 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3421 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3481 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3541 ttgagtgagc tgataccg
//
