LOCUS       sequence-206300-        3912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-234
FEATURES             Location/Qualifiers
     source          1..3912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..56
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1835..1853)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1921..2025
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2247..3038
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3209..3797
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3698..3717
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
       61 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatcac
      121 tctgtggtct caaatggcgg gtatcaatag agcgggacca tcgggcgctt attttgttgg
      181 ccacacagac cccgagccag tatcggggca agcacacgga tccggcagcg gcgccagctc
      241 ctcgaacagt ccgcaggttc agccgcgacc ctcgaatact cccccgtcga acgcgcccgc
      301 accgccgcca accggacgtg agaggctttc acgatccacg gcgctgtcgc gccaaaccag
      361 ggagtggctg gagcagggta tgcctacagc ggaggatgcc agcgtgcgtc gtaggccaca
      421 ggtgactgcc gatgccgcaa cgccgcgtgc agaggcaaga cgcacgccgg aggcaactgc
      481 cgatgccagc gcaccgcgta gaggggcggt tgcacacgcc aacagtatcg ttcagcaatt
      541 ggtcagtgag ggcgctgata tttcgcatac tcgtaacatg ctccgcaatg caatgaatgg
      601 cgacgcagtc gctttttctc gagtagaaca gaacatattt cgccagcatt tcccgaacat
      661 gcccatgcat ggaatcagcc gagattcgga actcgctatc gagctccgtg gagcgcttcg
      721 tcgagcggtt caccaacagg cggcgtcagc gccagtgagg tcgcccacgc caacaccggc
      781 cagccctgcg gcatcatcat cgggcagcag tcagcgttct ttatttggac ggtttgcccg
      841 tttgatggcg ccaaaccagg gacggtcgtc gaacactgcc gcctctcaga cgccggtcga
      901 caggagcccg ccacgcgtca accaaagacc catacgcgtc gacagggctg cgatgcgtaa
      961 tcgtggcaat gacgaggcgg acgccgcgct gcgggggtta gtacaacagg gggtcaattt
     1021 agagcacctg cgcacggccc ttgaaagaca tgtaatgcag cgcctcccta tccccctcga
     1081 tataggcagc gcgttgcaga atgtgggaat taacccaagt atcgacttgg gggaaagcct
     1141 tgtgcaacat cccctgctga atttgaatgt agcgttgaat cgcatgctgg ggctgcgtcc
     1201 cagcgctgaa agagcgcctc gtccagccgt ccccgtggct cccgcgaccg cctccaggcg
     1261 accggatggt acgcgtgcaa cacgattgcg ggtgatgccg gagcgggagg attacgaaaa
     1321 taatgtggcc tatggagtgc gcttgcttaa cctgaacccg ggggtggggg taaggcaggc
     1381 tgttgcggcc tttgtaaccg accgggctga gcggccagca gtggtggcta atatccgggc
     1441 agccctggac cctatcgcgt cacaattcag tcagctgcgc acaatttcga aggccgatgc
     1501 tgaatctgaa gagctgggtt ttaaggatgc ggcagatcat cacacggatg acgtgacgca
     1561 ctgtcttgcg ggcggagaat tgtcgctgag taatccggat cagcaggtga tcggtttggc
     1621 gggtaatccg acggacacgt cgcagcctta cagccaagag ggaaataagg acctggcgtt
     1681 catggatatg aaaaaacttg cccaattcct cgcaggcaag cctgagcatc cgatgaccag
     1741 agaaacgctt aacgccgaaa atatcgccaa gtatgctttt agaatagtcc cctgagcttt
     1801 gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag
     1861 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     1921 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1981 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac
     2041 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     2101 tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2161 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2221 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
     2281 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2341 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg
     2401 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2461 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2521 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2581 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2641 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2701 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     2761 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     2821 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     2881 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     2941 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccatt
     3001 acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa gtttactcat
     3061 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3121 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3181 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3241 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3301 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3361 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3421 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3481 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3541 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3601 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3661 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3721 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3781 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3841 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3901 ccgcctttga gt
//
