LOCUS       sequence-206302-        2522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pTEI042
FEATURES             Location/Qualifiers
     source          1..2522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        735..781
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(847..865)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        933..1037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1259..2050
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2221..2522,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagct
      541 tgcatgccgg tcgactctag aggatccggg tgacagccct ccgacgggtg acagccctcc
      601 gacgggtgac agccctccga attctagagg atccgggtga cagccctccg acgggtgaca
      661 gccctccgac gggtgacagc cctccgaatt cgagctcggt acccggggat ctgtcgacct
      721 cgatcgagat cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
      781 acacgctgaa gctagtcgac tctagccaat gtgagaccac gaagtggctc ttcagtggac
      841 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      901 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      961 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1021 attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1081 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca
     1141 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     1201 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     1261 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     1321 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     1381 cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     1441 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     1501 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     1561 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     1621 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     1681 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     1741 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     1801 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     1861 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     1921 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     1981 gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt
     2041 cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2101 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2161 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2221 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2281 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2341 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2401 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2461 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2521 gg
//
