LOCUS       sequence-206318-       11729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses acoustic reporter genes or gas vesicles (ARG2) in 
            Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Bourdeau RW, Lee-Gosselin A, Lakshmanan A, Farhadi A, Kumar SR, Nety
            SP, Shapiro MG
  TITLE     Acoustic reporter genes for noninvasive imaging of microorganisms in
            mammalian hosts.
  JOURNAL   Nature. 2018 Jan 3;553(7686):86-90. doi: 10.1038/nature25021.
  PUBMED    29300010
REFERENCE   2  (bases 1 to 11729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2018-01-3- 3"; volume: "553"; issue: "7686"; pages:
            "86-90"
COMMENT     Sequence Label: pET28a_T7-ARG2
FEATURES             Location/Qualifiers
     source          1..11729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(559..1374)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(559..1374)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1282..1301
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1366..1385
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1496..2084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1985..2004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2238..2255
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2270..2409
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2335..2354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2495..2517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2511..2702)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3511..4593)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4555..4574
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4594..4671)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4877..4896
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4980..4999
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4980..4998
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4999..5023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             7909..7926
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(11643..11661)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11657..11704
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
      481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      541 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
      601 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      661 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      721 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
      781 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
      841 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
      901 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
      961 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     1021 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     1081 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     1141 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     1201 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     1261 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     1321 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     1381 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     1441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1621 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     2281 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     2341 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2401 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2521 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     2581 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     2641 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     2701 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     2761 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     2821 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     2881 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     2941 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     3001 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     3061 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     3121 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
     3181 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     3241 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     3301 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     3361 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     3421 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     3481 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3541 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3601 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     3661 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     3721 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     3781 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     3841 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     3901 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     3961 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     4021 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     4081 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     4141 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     4201 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     4261 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     4321 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     4381 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     4441 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     4501 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     4561 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     4621 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     4681 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     4741 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     4801 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     4861 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     4921 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
     4981 aatacgactc actatagggg aattgtgagc ggataacaat tccaagcttc gccagggttt
     5041 tcccagtcga gctcatgact catatttata gttattctcc tcatttccca agtttccgac
     5101 aacaaataac aaggaattaa aaaaatggca gttgaaaaaa ccaattcttc ctccagcttg
     5161 gcagaagtta ttgatagaat cctcgacaaa ggtatcgtaa ttgacgcttg ggttcgtgtt
     5221 tccttagttg gtatcgaact actagcaatt gaagctcgga tcgttatcgc ttccgttgaa
     5281 acctacttga agtatgctga agcagttggt ttgacccaat cagcagcagt acctgcttaa
     5341 tttaattaag caagttacca agtaggagaa taaccatgac tcatatttat agttattctc
     5401 ctcatttccc aagtttccga caacaaataa caaggaatta aaaaaatggc agttgaaaaa
     5461 accaattctt cctccagctt ggcagaagtt attgatagaa tcctcgacaa aggtatcgta
     5521 attgacgctt gggttcgtgt ttccttagtt ggtatcgaac tactagcaat tgaagctcgg
     5581 atcgttatcg cttccgttga aacctacttg aagtatgctg aagcagttgg tttgacccaa
     5641 tcagcagcag tacctgctta atttaattaa gctccataat atcaggatta attaatcctg
     5701 atattggttc tgcttttttt aattatctag atatttggaa atcaacgctt ctcaaaggat
     5761 attacaaaaa agtacgaatc cttataatgt agtacctgga gaagcaattt ctgttaggtc
     5821 tgggagctcc catgggatcg ttatcccgcg gccaagcttg ctagcgctaa tttactattt
     5881 tttggagaac ttcatgattt ctttaatggc aaaaatccgg caagaacatc agtcaatagc
     5941 agagaaagtg gctgaactat ctcttgagac cagagaattc ttgtccgtca cgacagcgaa
     6001 aagacaagag caagctgaaa aacaagctca agaactgcaa gcattccgtc aggatttgtt
     6061 tgtgagtatc tttggttaat aaagatctaa ctattggagg ctactaaaaa tggaaattaa
     6121 aaaaattatg caagccgtga acgacttttt cggtgaacac gtagctcctc ctcataaaat
     6181 tacctcggtg gaagctactg aagatgaagg ttggagagtt attgttgaag tcattgaaga
     6241 acgagaatat atgaaaaaat acgccaaaga tgaaatgctc ggaacgtacg agtgctttgt
     6301 aaataaagaa aaagaagtca tttcattcaa acgactcgac gtcagatata gaagcgccat
     6361 tggcattgaa gcataaacga taagatggca ggaggaacgt aaaaatgacc gtcttaacag
     6421 acaaaaggaa aaaaggcagt ggagctttta tacaagatga cgagacaaaa gaggttcttt
     6481 caagagcgct gagctattta aaatccggct attccattca ttttacaggt cctgccggcg
     6541 gaggcaaaac ctctttagcg cgagcgcttg ctaaaaagag aaagcgtcct gtaatgctga
     6601 tgcacgggaa tcacgagctc aacaacaaag atttaattgg cgattttacg ggatacacga
     6661 gcaaaaaagt aatcgaccag tacgttcgtt ctgtctataa aaaagatgaa caggtgagtg
     6721 aaaactggca ggatggccga ttgcttgaag ctgtaaaaaa tggctatacg ctgatttacg
     6781 acgaatttac tcgttctaag cctgcgacga ataatatctt tctatcgata ttagaagaag
     6841 gcgtgctgcc gctgtatgga gtaaaaatga ccgatccttt tgtgcgcgtg catcccgatt
     6901 tccgcgtcat cttcacaagc aatccagctg agtatgccgg cgtatatgat acgcaagatg
     6961 cgcttctcga caggttaatt accatgttta ttgattataa agacatcgac agagagacag
     7021 cgattttaac ggagaaaacg gacgtagaag aagatgaagc gcgcacaatt gtaacgctcg
     7081 tagcaaacgt gcgaaaccgc tctggagacg aaaacagcag cggacttagc ctgcgggctt
     7141 cgcttatgat cgctaccctt gccacgcagc aagacattcc tatcgatgga agtgacgaag
     7201 attttcaaac gttatgtatc gatattttgc atcatccgct taccaaatgt ttggatgaag
     7261 aaaatgcaaa aagcaaagcc gaaaaaatca ttttagaaga atgtaagaat atagacactg
     7321 aagaaaagta aaggagcttg aaaacatgag tgaaacaaac gaaacaggta tttatatttt
     7381 tagcgccatt caaacggata aagacgaaga atttggcgcc gtggaagtag aaggaacaaa
     7441 agctgaaaca tttttgattc gctacaaaga cgcggctatg gtagcagctg aagtaccgat
     7501 gaaaatttat catcctaatc gccaaaattt attaatgcat caaaacgcag tagcagcgat
     7561 tatggacaag aacgatacgg ttattccaat cagctttggg aatgtattca aatcaaaaga
     7621 agacgtaaaa gttcttttgg aaaaccttta tccgcagttt gaaaagctgt ttccagcgat
     7681 caaaggaaaa attgaagtcg gtttaaaagt aattgggaaa aaagaatggc ttgagaaaaa
     7741 agtaaacgaa aatcctgaac ttgagaaagt atcagcatcc gtaaaaggaa aatcagaagc
     7801 agccggttat tatgagcgta ttcaacttgg aggaatggct caaaagatgt ttacttccct
     7861 gcaaaaagaa gtcaagacag atgtgttttc tccgcttgaa gaagcagcgg aagcagcaaa
     7921 agcaaatgag ccaacgggcg aaacgatgct tttaaacgcg tctttcttaa ttaaccgaga
     7981 agatgaagcg aagtttgatg aaaaagtaaa tgaagcgcat gaaaactgga aagacaaagc
     8041 cgattttcat tacagcggtc cttggcctgc ttataatttt gtgaacattc gcctaaaagt
     8101 agaagagaaa taacgtgctt cacaaattag taaccgcacc cattaacctt gtagtgaaaa
     8161 tcggcgaaaa agtacaggaa gaagctgata aacagctata tgaccttccg acgattcagc
     8221 aaaagctcat tcagcttcaa atgatgtttg agcttggtga aattccagaa gaagcgtttc
     8281 aagaaaaaga agatgaattg ttaatgaggt acgaaattgc gaaacgcaga gaaattgaac
     8341 aatgggaaga gctaacacaa aaaagaaatg aggaatccta gatgggagaa ttactgtatt
     8401 tatacggttt aattccaaca aaagaagcag cagccataga gccgtttcca tcttataagg
     8461 ggtttgacgg agaacattca ctgtacccaa ttgcgtttga tcaggtgacg gctgtagttt
     8521 ctaagctgga tgctgacacc tattcagaaa aagtgattca agaaaaaatg gagcaggata
     8581 tgagctggct gcaggaaaaa gcatttcatc atcacgaaac ggtagccgct ttgtacgaag
     8641 aatttacgat cattccatta aaattttgca ccatttataa aggtgaagaa agtctgcagg
     8701 cagctattga gattaacaaa gaaaagatag agaattcact gacgctgctt caaggaaatg
     8761 aagagtggaa tgtgaaaatt tactgtgatg atacagagct taaaaaagga atcagcgaaa
     8821 cgaatgaaag cgtgaaagcg aaaaaacaag aaattagtca cttatcacca ggaagacagt
     8881 tttttgaaaa gaaaaaaata gatcagctga ttgaaaaaga attagagctt cacaaaaaca
     8941 aagtgtgtga agagatacat gacaagctaa aagaattatc gctttatgac tctgttaaaa
     9001 agaattggag caaagacgta actggcgcag ctgaacagat ggcgtggaac agcgtgtttc
     9061 ttctcccgtc tctgcagatt actaagttcg taaacgaaat agaagagctt cagcaaaggc
     9121 ttgaaaataa aggctggaag tttgaagtga cgggaccatg gccgccctat catttctcga
     9181 gctttgcgta aagtgaggaa ttaacattat gtctcttaaa caatccatgg agaataaaga
     9241 tattgctctt attgatattt tagatgtcat tttagataaa ggagtcgcca ttaaaggaga
     9301 cttaatcatt tccatagctg gcgtcgattt agtgtatttg gatttgcggg tgcttatttc
     9361 ttcggttgaa acgcttgtgc aagcaaaaga aggaaatcac aaaccaatca cttctgaaca
     9421 atttgataaa caaaaggagg aattaatgga tgcaaccggt cagccaagca aatggacgaa
     9481 tccacttgga tcctgatcaa gctgaacaag gcttagcgca gcttgtgatg acagttattg
     9541 agctattgag gcaaatcgtt gaacgtcatg ccatgaggcg ggtggagggt ggaacgttga
     9601 cggacgaaca aattgaaaac ttaggaattg cactaatgaa cttagaagaa aaaatggacg
     9661 agttgaaaga ggtgttcggt ctggatgcag aagatttaaa tattgatctt ggaccgctag
     9721 gcagcctgct ttaagcggtc agtaggagga acagtatggc agtcgaacat aatatgcagt
     9781 caagtacgat tgtagatgtg ctcgaaaaga ttttggataa aggagtcgtt atagcggggg
     9841 acatcaccgt aggaattgca gatgtcgagc tattaacgat aaagatccgc ttgattgtgg
     9901 cttcggttga taaggcaaaa gaaatcggca tggactggtg ggaaaatgat ccgtatctca
     9961 gttcaaaagg agccaataac aaagcgctcg aagaagaaaa taaaatgctg catgagcggt
    10021 taaaaacgct tgaagaaaaa atagaaacga aacgttaaaa actgtacgct acttaaaaaa
    10081 tggagggatt tacaatggca actgaaacaa aattagataa cacacaggca gaaaacaagg
    10141 aaaataaaaa tgcggaaaac ggttcaaaag aaaagaacgg ttcaaaagca agcaaaacaa
    10201 caagcagcgg gccaatcaaa cgagcggtag caggaggcat catcggtgca acgattggat
    10261 atgtatcgac tcctgaaaat cgaaaaagtc tccttgaccg cattgataca gacgaattaa
    10321 aaagcaaagc atctgattta ggaacaaagg taaaagaaaa atcaaaaagc agcgtggcca
    10381 gcctgaaaac atctgcggga agcttgttta aaaaagataa agataaatca aaagatgatg
    10441 aagaaaacgt aaattcttct agtagcgaaa cagaagacga taacgttcaa gagtacgacg
    10501 agttaaaaga agaaaatcaa actcttcaag atcgcttatc acagcttgaa gaaaaaatga
    10561 acatgcttgt tgagcttagc ctcaataaaa atcaagacga agaagcggaa gatacagatt
    10621 ccgacgaaga agagaacgat gagaacgatg aaaacgatga aaacgagcag gacgatgaaa
    10681 acgaagaaga aacatctaag ccacgtaaaa aggataaaaa agaagctgag gaagaagaaa
    10741 gtgaaagtga cgaagacagc gaggaagaag aggaagattc tcgctcaaac aaaaaaaata
    10801 aaaaagtaaa aacagaagaa gaagacgaag atgaaagcga agaagaaaaa aaggaagcga
    10861 aaccaaaaaa gtcaacagct aaaaaatcaa aaaatacaaa agcaaagaaa aacacggacg
    10921 aagaagatga tgaagcaaca tctctttcta gtgaagacga tacaacagcc taagacgtaa
    10981 aggaggaaag aaagacatga gtacaggccc ttctttttca actaaagaca atacgcttga
    11041 atactttgtg aaagcttcta ataaacacgg cttttcactt gatatttcat taaatgtaaa
    11101 cggcgctgtg atttccggta ccatgatttc agcaaaagaa tactttgatt acttaagcga
    11161 aacgtttgaa gaaggcagtg aagtggctca ggcgctaagc gaacaattct ctttagcaag
    11221 cgaagcgagc gaatcaaacg gagaagcaga agcccatttt attcatttga aaaatacaaa
    11281 gatttactgt ggagacagta aatctactcc ttctaaaggc aaaatctttt ggagagggaa
    11341 aatagcagaa gtagacgggt ttttcttagg aaagatttct gatgcaaaat caacgagtaa
    11401 aaagagttca taaaaaacgg cggggtgatt gccccgccgt tttttagtga tgtgatgaga
    11461 tgtgcagctt cttttttcga tacatgagcg gagtcatctt catttctttt cgaaactgat
    11521 taataaaata gcttgtacta ttaaacccta cttgataggc gacttccgtc acatttgctt
    11581 ctgcttgctg caggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
    11641 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
    11701 tttgctgaaa ggaggaacta tatccggat
//
