LOCUS       sequence-206335-       14008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses epitope-tagged wild-type murine TTP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14008)
  AUTHORS   Coelho MA, de Carne Trecesson S, Rana S, Zecchin D, Moore C, 
            Molina-Arcas M, East P, Spencer-Dene B, Nye E, Barnouin K, Snijders 
            AP, Lai WS, Blackshear PJ, Downward J
  TITLE     Oncogenic RAS Signaling Promotes Tumor Immunoresistance by 
            Stabilizing PD-L1 mRNA.
  JOURNAL   Immunity. 2017 Dec 19;47(6):1083-1099.e6. doi: 
            10.1016/j.immuni.2017.11.016. Epub 2017 Dec 12.
  PUBMED    29246442
REFERENCE   2  (bases 1 to 14008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2017.11.016"; journalName: "Immunity"; date: 
            "2017-12-19- 19"; volume: "47"; issue: "6"; pages: "1083-1099.e6"
COMMENT     Sequence Label: pTRIPZ Myc2-mouse TTP WT
FEATURES             Location/Qualifiers
     source          1..14008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3032..3061
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3062..3091
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(4051..4062)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        4758..5969
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5806..5825
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5982..6015)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             6022..6729
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(6730..6763)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    6786..7358
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6953..6970)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7180..7199
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7361..7963
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7860..7880
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7979..8567
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8032..8052)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8450..8461)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8774..9007
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9108..9125)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9114..9338
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9384..9812
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9471..9490)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9681..9702
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9821..9841)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9826..10155
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10006..10141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10068..10087
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    11363..11484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11533..11549)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11533..11549)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11546..11568)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11557..11573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11581..11587,11588..11605,11606..11611))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11626..11647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11764..11781)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11935..12523)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12015..12034)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12694..13485,13486..13554))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13317..13336
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13555..13659)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13707..13841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtgct
     3001 agcgtttaaa cttaagcttg gtaccaccat ggagcagaag ctgatctcag aggaggatct
     3061 ggagcagaag ctgatctcag aggaggatct ggatatccag cacagtggcg gccgcgatct
     3121 ctctgccatc tacgaggtga gtacctgtta gacagcatcc cgggatcccc gacgcaccaa
     3181 acttaggccc gggacgcctc cgtaccttct cagactagcc ctggctggct ggaaatgaga
     3241 gagggagcca gcgagcctag cgcctcggtc cagataccgg cgatcccctt gggacgaaaa
     3301 gctggcacac tgctgtcact cgcgtagggc ggaagcggca tcacacctgt ctagtgccct
     3361 tcgctgccca tggcgcggtt tggtggccaa gcaggtgcta aggttgaggt agggggggag
     3421 ttgacagggt aagagttcta gagagatctg gcctcctggg tcagctttgg ccggctcctt
     3481 caagttgtga aacgagcgcc cggtgggcgg tcatatgggg gggggggagt cctagaggcc
     3541 caaagttggc gagcagcagt gtgctttgat ttggggggga accctctctc gatcggggat
     3601 actgggcttc ctagatcctt ctggattacc ttcagtcatg tggaaacact caaatctctg
     3661 tagtcaggtt ctccctggag tttgtgtgag ccgggtgggg ctcaggccga gaacccacaa
     3721 accggactgg caagctcgtg aagtgcttta caacaggggc ggggcgacgt caccttacct
     3781 tttctggtgg cctaaccgaa ccctgttcct ctcagagcct ccagtcgatg agccatgacc
     3841 tgtcatccga ccacggagga accgaatccc tcggaggact ttggaacata aactcggact
     3901 ccatcccgtc tggggtcacc tctcgcctga ctggccgctc cactagcctg gtggagggcc
     3961 gaagctgtgg ctgggtaccc ccaccccctg gttttgcacc tttggctccc cgcccaggcc
     4021 ctgagctgtc accctcacct acttcgccta ctgcaactcc caccacctcc tctcgataca
     4081 agaccgagct ctgtcggacc tactcagaaa gcgggcgttg tcgctacggg gccaagtgcc
     4141 agtttgctca cggcctgggt gaacttcgcc aagccaatcg ccaccccaag tacaaaacgg
     4201 aactctgcca caagttctac ctccagggcc gctgccccta tggctctcga tgccacttca
     4261 tccacaaccc caccgaggac ctagctctcc ctggccagcc ccatgtgctg cgacaaagca
     4321 tcagcttctc cggcttgccc tcaggccgca gaagctcgcc gccacctcca ggcttttctg
     4381 gcccttccct gtcctcttgt tccttttcgc cttccagctc cccaccgccc cctggggatc
     4441 ttccactttc cccttctgcc ttctctgctg cccctgggac ccctgtgact cgaagagacc
     4501 ctaaccaggc ctgttgcccc tcctgccgaa ggtctactac ccccagcacc atctgggggc
     4561 ccttgggtgg cctggctcgg agcccatctg cccactctct gggatccgat cctgatgact
     4621 acgccagcag cggcagcagc ctgggggggt cagactcacc tgtctttgag gcaggggtgt
     4681 ttgggcctcc ccagacccct gcacccccaa ggcgtctccc catcttcaat cgtatctctg
     4741 tctctgagtg aacgcgtggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
     4801 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc
     4861 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca
     4921 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga
     4981 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg
     5041 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
     5101 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag
     5161 tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa
     5221 gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact
     5281 gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct
     5341 tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
     5401 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
     5461 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
     5521 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
     5581 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
     5641 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     5701 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     5761 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     5821 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     5881 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
     5941 tctggccgtt tttggctttt ttgttagacg ctagcggatc cataacttcg tatagtatac
     6001 attatacgaa gttatgccac catgtctagg ctggacaaga gcaaagtcat aaacggagct
     6061 ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     6121 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     6181 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
     6241 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat
     6301 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     6361 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     6421 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     6481 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca
     6541 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     6601 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac
     6661 gattttgact tagacatgct cccagccgat gcccttgacg attttgacct tgacatgctc
     6721 cccgggtaaa taacttcgta tagtatacat tatacgaagt tatggatccg cggccgcaaa
     6781 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     6841 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     6901 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     6961 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     7021 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     7081 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     7141 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     7201 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     7261 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     7321 cacggggacg tggttttcct ttgaaaaaca cgataatacc atggccaccg agtacaagcc
     7381 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     7441 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     7501 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     7561 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     7621 ggtgttcgcc gagatcggct cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     7681 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     7741 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     7801 cggagtggag gcggccgagc gcgctggggt gcccgccttc ctggagacct ccgcgccccg
     7861 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7921 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagttcgcg tctggaacaa
     7981 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     8041 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     8101 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     8161 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     8221 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     8281 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     8341 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     8401 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     8461 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     8521 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     8581 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     8641 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     8701 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     8761 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     8821 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8881 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8941 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9001 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     9061 atatcagaga gtgagaggcc ttgacattgt ttaaacccgc tgatcagcct cgactgtgcc
     9121 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     9181 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     9241 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     9301 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     9361 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     9421 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     9481 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     9541 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     9601 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     9661 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     9721 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     9781 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     9841 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9901 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9961 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    10021 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    10081 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    10141 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
    10201 gtgatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
    10261 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
    10321 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
    10381 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
    10441 attggggaat taattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
    10501 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
    10561 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
    10621 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
    10681 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
    10741 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
    10801 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
    10861 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
    10921 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
    10981 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
    11041 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
    11101 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
    11161 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
    11221 ggcaaaggaa tagcacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
    11281 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    11341 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
    11401 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11461 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
    11521 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    11581 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    11641 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    11701 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    11761 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    11821 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11881 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11941 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    12001 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    12061 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    12121 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    12181 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    12241 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    12301 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    12361 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    12421 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    12481 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    12541 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    12601 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    12661 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    12721 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    12781 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12841 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12901 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12961 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    13021 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    13081 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    13141 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    13201 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    13261 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    13321 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    13381 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    13441 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    13501 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    13561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    13621 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    13681 acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat
    13741 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    13801 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    13861 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    13921 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    13981 gttaaatatg tactacaaac ttagtagt
//
