LOCUS       sequence-206338-        2840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BSMT1 in Ecoli off a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, 
            Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ
  TITLE     BioBits Explorer: A modular synthetic biology education kit.
  JOURNAL   Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. 
            eCollection 2018 Aug.
  PUBMED    30083608
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-08-1"; volume: "4(8):eaat5105. doi"; pages: " 
            10.1126/sciadv.aat5105. eCollection 2018 Aug"
COMMENT     Sequence Label: pJL1 BSMT1
FEATURES             Location/Qualifiers
     source          1..2840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     617..636
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        617..635
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             667..689
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1825..1843)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1839..1886
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1904..2719)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2627..2646
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2711..2730
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga
      661 aataattttg tttaacttta agaaggagat atacatatgg aagttgttga agttcttcac
      721 atgaatggag gaaatggaga cagtagctat gcaaacaatt ctttggttca gcaaaaggtg
      781 attctcatga caaagccaat aactgagcaa gccatgattg atctctacag cagcctcttt
      841 ccagaaacct tatgcattgc agatttgggt tgttctttgg gagctaacac tttcttggtg
      901 gtctcacagc ttgttaaaat agtagaaaaa gaacgaaaaa agcatggttt taagtctcca
      961 gagttttatt ttcacttcaa tgatcttcct ggcaatgatt ttaatacact ttttcagtca
     1021 ctgggggcat ttcaagaaga tttgagaaag catatagggg aaagctttgg tccatgtttt
     1081 ttcagtggag tgcctggttc attttatact agacttttcc cttccaaaag tttacatttt
     1141 gtttactcct cctacagtct catgtggcta tctcaggtgc ctaatgggat tgaaaataac
     1201 aagggaaaca tttacatggc aagaacaagc cctctaagtg ttattaaagc atactacaag
     1261 caatatgaaa tagatttttc aaattttctc aagtaccgtt cagaggaatt gatgaaaggt
     1321 ggaaagatgg tgttaacact cctaggtaga gaaagtgagg atcctactag caaagaatgc
     1381 tgttacattt gggagcttct agccatggcc ctcaataagt tggttgaaga gggattgata
     1441 aaagaagaga aagtagatgc attcaatatt cctcaataca caccatcacc agcagaagta
     1501 aagtacatag ttgagaagga aggatcattc accattaatc gcttggaaac atcaagagtt
     1561 cattggaatg cttctaataa tgagaagaat ggtggttaca atgtgtcaag gtgcatgaga
     1621 gctgtggctg agcctttgct tgtcagccac tttgacaagg aattgatgga tttagtgttc
     1681 cacaagtacg aagagattgt ttctgattgc atgtccaaag agaatactga gtttataaat
     1741 gtcatcatct ccttgaccaa aataaattaa taagtcgacc ggctgctaac aaagcccgaa
     1801 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     1861 ctaaacgggt cttgaggggt tttttgctga aagccaattc tgattagaaa aactcatcga
     1921 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     1981 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     2041 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     2101 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     2161 gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     2221 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     2281 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     2341 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     2401 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     2461 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     2521 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     2581 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     2641 tatacccata taaatcagca tccatgttgg aatttaatcg cggcttcgag caagacgttt
     2701 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     2761 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
     2821 cgtgagatca aaggatcttc
//
