LOCUS       sequence-206340-        2837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Trx-Bx in Ecoli off a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, 
            Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ
  TITLE     BioBits Explorer: A modular synthetic biology education kit.
  JOURNAL   Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. 
            eCollection 2018 Aug.
  PUBMED    30083608
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-08-1"; volume: "4(8):eaat5105. doi"; pages: " 
            10.1126/sciadv.aat5105. eCollection 2018 Aug"
COMMENT     Sequence Label: pJL1 Trx-Bx
FEATURES             Location/Qualifiers
     source          1..2837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     617..636
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        617..635
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             667..689
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1045..1062
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1822..1840)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1836..1883
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1901..2716)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2624..2643
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2708..2727
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga
      661 aataattttg tttaacttta agaaggagat atacatatga gtgataagat tatccatctt
      721 actgacgact ccttcgatac cgacgtcctg aaagcggatg gagctatcct tgtggacttt
      781 tgggctgagt ggtgtggccc atgtaaaatg atcgccccga ttctggatga gattgcggac
      841 gaatatcaag gaaagttgac cgttgctaag cttaacattg accaaaatcc aggaaccgct
      901 ccaaaatacg gtattcgtgg catcccaacg cttttgcttt tcaaaaatgg tgaagttgcg
      961 gctaccaaag taggtgccct gtcaaaaggt caattaaaag aatttctgga tgcgaacttg
     1021 gcaggttctg gttctggcca catgcaccac catcatcatc atagcagtgg tgtaattggg
     1081 ggggacgagt gcgacatcaa cgagcacccg ttcttagcat tcatgtatta ttctccgcgc
     1141 tacttctgtg gtatgacgct gattaaccaa gagtgggtat tgactgctgc acactgtaac
     1201 cgtcgtttca tgcgcattca cctgggaaag catgcaggga gcgtggctaa ttatgatgaa
     1261 gttgttcgtt atcctaaaga aaaatttatt tgtccaaaca agaaaaaaaa cgtgattacc
     1321 gacaaggata ttatgctgat ccgtttggac cgccctgtca aaaattcgga acacattgcc
     1381 ccattgagcc tgccctcaaa ccccccctcc gtagggtcgg tgtgtcgtat tatggggtgg
     1441 ggtgcaatca cgacgtcgga agatacgtat cccgatgtac cacattgcgc taacatcaat
     1501 cttttcaaca atacagtttg ccgcgaagcg tataacgggc tgcccgcgaa gactctttgt
     1561 gcgggcgtct tgcagggtgg cattgacaca tgtggcggtg actcgggagg tccgttaatt
     1621 tgtaatgggc agtttcaagg aatcttgagc tggggctcgg acccatgtgc ggaacctcgc
     1681 aaacccgcat tttatacaaa ggtattcgat taccttccgt ggatccagtc gattatcgcc
     1741 gggaacaaga ctgcaacttg tccttaataa gtcgaccggc tgctaacaaa gcccgaaagg
     1801 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     1861 aacgggtctt gaggggtttt ttgctgaaag ccaattctga ttagaaaaac tcatcgagca
     1921 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     1981 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     2041 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     2101 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     2161 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     2221 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     2281 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     2341 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     2401 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     2461 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     2521 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     2581 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     2641 acccatataa atcagcatcc atgttggaat ttaatcgcgg cttcgagcaa gacgtttccc
     2701 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     2761 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt
     2821 gagatcaaag gatcttc
//
