LOCUS       sequence-206353-        3676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG640
FEATURES             Location/Qualifiers
     source          1..3676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     455..472
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1542..1887
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1830..1850
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(2015..2033)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2101..2205
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2427..3218
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3389..3676,1..301)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
       61 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      481 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactctg
      541 tggtctcagg aggaattcca atcccacaaa aatctgagct taacagcaca gttgctcctc
      601 tcagagcaga atcgggtatt caacaccctc atatcaacta ctacgttgtg tataacggtc
      661 cacatgccgg tatatacgat gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg
      721 gagttgcaca caagaaattt gccactatta cagaggcaag agcagcagct gacgcgtaca
      781 caacaagtca gcaaacagac aggttgaact tcatccccaa aggagaagct caactcaagc
      841 ccaagagctt tgctaaggcc ctaacaagcc caccaaagca aaaagcccac tggctcacgc
      901 taggaaccaa aaggcccagc agtgatccag ccccaaaaga gatctccttt gccccggaga
      961 ttacaatgga cgatttcctc tatctttacg atctaggaag gaagttcgaa ggtgaaggtg
     1021 acgacactat gttcaccact gataatgaga aggttagcct cttcaatttc agaaagaatg
     1081 ctgacccaca gatggttaga gaggcctacg cagcaagtct catcaagacg atctacccga
     1141 gtaacaatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     1201 tcaggactaa ttgcatcaag aacacagaga aagacatatt tctcaagatc agaagtacta
     1261 ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag
     1321 tctctaaaaa ggtagttcct actgaatcta aggccatgca tggagtctaa gattcaaatc
     1381 gaggatctaa cagaactcgc cgtcaagact ggcgaacagt tcatacagag tcttttacga
     1441 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct ggtctactcc
     1501 aaaaatgtca aagatacagt ctcagaagat caaagggcta ttgagacttt tcaacaaagg
     1561 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     1621 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     1681 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     1741 gtggaaaaag aagaggttcc aaccacgtct acaaagcaag tggattgatg tgacatctcc
     1801 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     1861 ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctcattt ttacaacaat
     1921 taccaacaac aacaaacaac aaacaacatt acaattacat ttacaattat cgataccatt
     1981 gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag
     2041 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     2101 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2161 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac
     2221 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     2281 tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2341 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2401 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
     2461 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2521 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg
     2581 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2641 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2701 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2761 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2821 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2881 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     2941 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     3001 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     3061 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     3121 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccatt
     3181 acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa gtttactcat
     3241 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3301 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3361 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3421 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3481 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3541 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3601 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3661 tggactcaag acgata
//
