LOCUS       sequence-206355-        3912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG603
FEATURES             Location/Qualifiers
     source          1..3912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2237..2255)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2323..2427
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2649..3440
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3611..3912,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagct
      541 caacttttga ttcgctattt gcagtgcacc tgtggcgttc atcacatctt ttgtgacact
      601 gtttgcactg gtcattgcta ttacaaagga ccttcctgat gttgaaggag atcgaaagta
      661 agtaactgca cgcataacca ttttctttcc gctctttggc tcaatccatt tgacagtcaa
      721 agacaatgtt taaccagctc cgtttgatat attgtcttta tgtgtttgtt caagcatgtt
      781 tagttaatca tgcctttgat tgatcttgaa taggttccaa atatcaaccc tggcaacaaa
      841 acttggagtg agaaacattg cattcctcgg ttctggactt ctgctagtaa attatgtttc
      901 agccatatca ctagctttct acatgcctca ggtgaattca tctatttccg tcttaactat
      961 ttcggttaat taaagcacga acaccattac tgcatgtaga agcttgataa actatcgcca
     1021 ccaatttatt tttgttgcga tattgttact ttcctcagta tgcagctttg aaaagaccaa
     1081 ccctcttatc ctttaacaat gaacaggttt ttagaggtag cttgatgatt cctgcacatg
     1141 tgatcttggc ttcaggctta attttccagg taaagcatta tgagatactc ttatatctct
     1201 tacatacttt tgagataatg cacaagaact tcataactat atgctttagt ttctgcattt
     1261 gacactgcca aattcattaa tctctaatat ctttgttgtt gatctttggt agacatgggt
     1321 actagaaaaa gcaaactaca ccaaggtaaa atacttttgt acaaacataa actcgttatc
     1381 acggaacatc aatggagtgt atatctaacg gagtgtagaa acatttgatt attgcaggaa
     1441 gctatctcag gatattatcg gtttatatgg aatctcttct acgcagagta tctgttattc
     1501 cccttcctct agctttcaat ttcatggtga ggatatgcag ttttctttgt atatcattct
     1561 tcttcttctt tgtagcttgg agtcaaaatc ggttccttca tgtacataca tcaaggatat
     1621 gtccttctga atttttatat cttgcaataa aaatgcttgt accaattgaa acaccagctt
     1681 tttgagttct atgatcactg acttggttct aaccaaaaaa aaaaaaatgt ttaatttaca
     1741 tatctaaaag taggtttagg gaaacctaaa cagtaaaata tttgtatatt attcgaattt
     1801 cactcatcat aaaaacttaa attgcaccat aaaattttgt tttactatta atgatgtaat
     1861 ttgtgtaact taagataaaa ataatattcc gtaagttaac cggctaaaac cacgtataaa
     1921 ccagggaacc tgttaaaccg gttctttact ggataaagaa atgaaagccc atgtagacag
     1981 ctccattaga gcccaaaccc taaatttctc atctatataa aaggagtgac attagggttt
     2041 ttgttcgtcc tcttaaagct tctcgttttc tctgccgtct ctctcattcg cgcgacgcaa
     2101 acgatcttca ggtgatcttc tttctccaaa tcctctctca taactctgat ttcgtacttg
     2161 tgtatttgag ctcacgctct gtttctctca ccacagcaat gtgagaccac gaagtggctc
     2221 ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     2281 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2341 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2401 cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc
     2461 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     2521 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     2581 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     2641 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     2701 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     2761 tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc
     2821 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2881 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2941 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     3001 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     3061 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     3121 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     3181 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     3241 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     3301 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     3361 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca
     3421 ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt
     3481 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3541 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3601 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3661 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3721 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3781 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3841 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3901 taccggataa gg
//
