LOCUS       sequence-206356-        3910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pTEI044
FEATURES             Location/Qualifiers
     source          1..3910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     455..472
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2249..2267)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2335..2439
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2661..3452
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3623..3910,1..301)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
       61 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      481 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactctg
      541 tggtctcagg agctcaactt ttgattcgct atttgcagtg cacctgtggc gttcatcaca
      601 tcttttgtga cactgtttgc actggtcatt gctattacaa aggaccttcc tgatgttgaa
      661 ggagatcgaa agtaagtaac tgcacgcata accattttct ttccgctctt tggctcaatc
      721 catttgacag tcaaagacaa tgtttaacca gctccgtttg atatattgtc tttatgtgtt
      781 tgttcaagca tgtttagtta atcatgcctt tgattgatct tgaataggtt ccaaatatca
      841 accctggcaa caaaacttgg agtgagaaac attgcattcc tcggttctgg acttctgcta
      901 gtaaattatg tttcagccat atcactagct ttctacatgc ctcaggtgaa ttcatctatt
      961 tccgtcttaa ctatttcggt taattaaagc acgaacacca ttactgcatg tagaagcttg
     1021 ataaactatc gccaccaatt tatttttgtt gcgatattgt tactttcctc agtatgcagc
     1081 tttgaaaaga ccaaccctct tatcctttaa caatgaacag gtttttagag gtagcttgat
     1141 gattcctgca catgtgatct tggcttcagg cttaattttc caggtaaagc attatgagat
     1201 actcttatat ctcttacata cttttgagat aatgcacaag aacttcataa ctatatgctt
     1261 tagtttctgc atttgacact gccaaattca ttaatctcta atatctttgt tgttgatctt
     1321 tggtagacat gggtactaga aaaagcaaac tacaccaagg taaaatactt ttgtacaaac
     1381 ataaactcgt tatcacggaa catcaatgga gtgtatatct aacggagtgt agaaacattt
     1441 gattattgca ggaagctatc tcaggatatt atcggtttat atggaatctc ttctacgcag
     1501 agtatctgtt attccccttc ctctagcttt caatttcatg gtgaggatat gcagttttct
     1561 ttgtatatca ttcttcttct tctttgtagc ttggagtcaa aatcggttcc ttcatgtaca
     1621 tacatcaagg atatgtcctt ctgaattttt atatcttgca ataaaaatgc ttgtaccaat
     1681 tgaaacacca gctttttgag ttctatgatc actgacttgg ttctaaccaa aaaaaaaaaa
     1741 atgtttaatt tacatatcta aaagtaggtt tagggaaacc taaacagtaa aatatttgta
     1801 tattattcga atttcactca tcataaaaac ttaaattgca ccataaaatt ttgttttact
     1861 attaatgatg taatttgtgt aacttaagat aaaaataata ttccgtaagt taaccggcta
     1921 aaaccacgta taaaccaggg aacctgttaa accggttctt tactggataa agaaatgaaa
     1981 gcccatgtag acagctccat tagagcccaa accctaaatt tctcatctat ataaaaggag
     2041 tgacattagg gtttttgttc gtcctcttaa agcttctcgt tttctctgcc gtctctctca
     2101 ttcgcgcgac gcaaacgatc ttcaggtgat cttctttctc caaatcctct ctcataactc
     2161 tgatttcgta cttgtgtatt tgagctcacg ctctgtttct ctcaccacac cattgagacc
     2221 acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2281 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2341 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2401 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc
     2461 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     2521 gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2581 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2641 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2701 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2761 acggctccgc agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg
     2821 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2881 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2941 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3001 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3061 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3121 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3181 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3241 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3301 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3361 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtaa
     3421 aaggcgagat caccaaggta gtcggcaaat aactgtcaga ccaagtttac tcatatatac
     3481 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3541 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3601 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3661 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3721 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3781 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3841 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3901 caagacgata
//
