LOCUS       sequence-206360-        4412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG295
FEATURES             Location/Qualifiers
     source          1..4412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaggagatg tcgccttaaa aaatgaatcg aatggtgagt gagacacctg
     2281 atattagttg tgtgaattca cgatgtttta ttagtttatt tccgtcattg gtgatctcct
     2341 actatatttg ttacggtatt agacatttaa ttggatttaa acaatttaat cacttacggt
     2401 atttacggta ttagaattat tattgtttga ttatatatat atatatatat attttttttt
     2461 tggtgtaaat gattttatat tttatcaaac ataatctggt tttatatttt ataaacatga
     2521 tatgattatt ttaaatttat aaaaactcta aaaactctaa aatctctatt aatattaaat
     2581 ttattaaata aattttaatt agttgacccg tgatccaacc tggttgactc gattacccag
     2641 tgacaaagaa agtagtccgg ttcattatcc aggtcgggtt taaaaacatt ggtttttaat
     2701 ttttggattt tggttttagg ttttcgattt ttgtagtttg tgatttttgt tgtagatttt
     2761 aatttttaca gaatcttaaa tggaaattta tttagttttt agttctctac tttaatttat
     2821 agttttttat tttttgttta aaaattacta aaacaatgaa aaattgttta aaaatcttga
     2881 atggaaattc gttaaaggtg gattgtcaaa gtcaatccat aactgaaatt agcaatttta
     2941 gggtaaatta gcaattttct gcgagggtag tagctgttta cccaaaatct tgaatggaaa
     3001 tttgtttcaa cgtttttaag ttctctattt tattatagtt ttttattttc tgtttaaaaa
     3061 ttactaaaac aatgataata aatattttct taataataaa aaattaaaaa gatgcagaaa
     3121 aaaaataatc ataggtatat tgcatataaa catagatttt gtaaataaaa ccaattttta
     3181 cgaaaaacta aaatgaaaaa ctcatattaa tacaataata ctaaaataat gatgtagaaa
     3241 agaaaaaaca tagcatattt aaaataataa aaaagaatta aaaaataaat atcacataaa
     3301 tcttctagaa atttggtttc cttatgtttt agaaaatcta ctttttatta atcataaatg
     3361 agtaattatt agtttttgat ttaatctacg tttttcttaa tcataaatgg gtaattatta
     3421 gtttttgcaa aatcaaaatc caaaaattgt tctaaacact gcaaccattt aaggcctata
     3481 tcactcagaa aatttctggt gggagaacta atcgtttgtc ctttctaaat ctcacatatt
     3541 agaatttaga attagtgtgc tacataaaaa tattagttca gctcggaaca actatttttt
     3601 ggtaaaacag agaacttaaa caaatgcatt attttatcaa catgcatttt gaattgaata
     3661 taaaatttca taattgtaaa gacataaatt acataaaatt ttacatgaaa aaatagatat
     3721 agaaagaaaa tgaaactaac tgatgatatg ctctctaaat tttttaatct cataacaaga
     3781 attcaaatta attagttcat atttttggtt aatataacat ttacctgtct aagttggaac
     3841 tttcattttt tttctgtttt gtttagtcag tattcttaat gtgaaacgga aagttgaatt
     3901 tattcaaact taaattcaat agcattaatt aaaggcgaaa gctattatct ctacatgtgg
     3961 ttcaaactag acatccaatt taattagctt attgacgttg aaatgttttc caaaactact
     4021 atagtttggc aatttgaaag atgcatcaga actactcaga caggtaaaag tagaacctct
     4081 agctgtgtga attgtatgtt agtccataaa gaacatcttg taaacttcat acttaagata
     4141 tatattacaa tatatacttg aatggtagat aaaaacgatt agtctgattg ctagcatact
     4201 cacaactatt tggaaatgag taagatattg gcatctagag ttactactat ggagacaaaa
     4261 gtcgaataaa agagtcctca cgtgaaaatg ttacgagcta gtaactaaag catttacact
     4321 aacggtaaaa aaagtatcta taaatgttta cacaaggtag tagtcattag cactcaactt
     4381 aaactctttt agtaacaatg tgagaccacg aa
//
