LOCUS       sequence-206367-        3234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG467
FEATURES             Location/Qualifiers
     source          1..3234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     455..472
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1573..1591)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1659..1763
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1985..2776
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2947..3234,1..301)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
       61 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      481 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactctg
      541 tggtctcagg agtgacgatg gttgttaaac gaaataattt aaatcaatgg agtttttata
      601 agtttgtaat gcgtttgttt ttgttatagt aatattgagt tggatctttg tttacgggac
      661 gtagaatact aaataatgaa aaaaaccttc tcgatgaatt aagggtttta tgaatttgtt
      721 ttgtattgaa taatataggg atggataaag ttttattatt ctaacaggtt actttattag
      781 gcatttcttc ggctcatgta actcttgtat cgctgaaact atgtaataga tagaagaacc
      841 taaaaaaaga aagaaaacaa gaaatgcaca tagcgaagct caaaagatga gtgttctgct
      901 agcggtaatg ttgttattca gttgggtcaa atgctctaat tgcaaatctt atttgggcct
      961 tatatagact cttatgtgca tatggtccag cctatttggg ccgatgtgtt tgaagatcat
     1021 ttgggaaagt cttgcgcaag gagaacttac ttgggacttg ggagacttgg agtaacaaat
     1081 tcgtcttgga gtaacaaata catgtcaact tattccacaa gttctttgtg ataatgatat
     1141 acatatttaa atgttttaaa aaagggaaag aataaacaaa ctctttgtga ctttttgggt
     1201 atttatgatt cttttggtcc ttctcttgta cacatttgag tcctatctta tatggtaagc
     1261 tttcaaatgc aagcgtgtta tttttattca cacttgttct atcttaatag tatgttgaac
     1321 tcttcgcctt tggcgattct tcccggaaaa gggaccattg actttcagat acacttcttt
     1381 ttttttttta taataattat tatcagttca atgatttttc aatatggact ctcgttctca
     1441 gacatttatt tctctcagtc ttacaatata aattttcatt cttaccatcc ataattttgt
     1501 attgtcttgt ccacagatct attccagctc acgccattga gaccacgaag tggctcttca
     1561 gtggacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     1621 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1681 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1741 aataatattg aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1801 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg
     1861 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     1921 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     1981 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     2041 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     2101 tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga
     2161 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     2221 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     2281 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     2341 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     2401 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     2461 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     2521 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     2581 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     2641 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     2701 ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa
     2761 ggtagtcggc aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2821 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2881 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2941 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3001 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3061 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3121 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3181 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gata
//
