LOCUS       sequence-206368-        3856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG648
FEATURES             Location/Qualifiers
     source          1..3856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2181..2199)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2267..2371
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2593..3384
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3555..3856,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagaa
      541 gataaatttg cgagtctaac agacggtgtg ttttcattca agatgataga tttgcaacta
      601 gttctatatc atctgatcaa ctatcaagta ttaaacattt atccatatga tataatcaag
      661 gaacacaaca ccatggataa aacacaaaac caaagcacag atccaattag tgacccaaca
      721 cgaagaaaat ttattcgcta tgagaatgaa gcattatgca tgtgtgaatt tacgccatag
      781 tctagttacc accgccccac cgcatgcgtg ttaatatcta ttttttgggc tcaagaaatt
      841 gcacggcatc aagtggaaga gacatgccaa attttaattt taaattagat gatccattgg
      901 aagcgaagtt caaaaacgaa attttacatt acatgatcgg tttgatcgat agtcgcttac
      961 aattgtggta aaaaagtgct agccattttg gccaagagtg taaaacgtac cgatcaaatg
     1021 tctttataaa aaaaacgtgt tgatgttgtt ctgtgaatac aattagttct ggttaacagc
     1081 tggtcgacca ttttctgatg agaatttatg taaggccatt gctctggtgt tgagaaggtt
     1141 tagtttggtt caagctaacc gtggttagaa agttagaata taatgtgttt cttgatcagt
     1201 gatatcgatc ggatttgtat tattcatatt gtttactctt tgagtaattc atagtggtaa
     1261 ctcttttttt tttttttttt tttttttcat attggtaact ctttgaaatg aaaaacatag
     1321 ctaagaattg ctagctttga tttagtcgag acgtacgaac tctcgatttt ggtttttgat
     1381 ttgttggtgt aaaactctcg atattcataa ctcgtaagat tttgtacgta tcatcttctt
     1441 attctcttca tcgctctgtt ttcaatttta tgtcaaaaca tggttttggt aatttctttt
     1501 actcctactt cacggtttga gttataattt ttttggtaaa cccttaacca cgagttttga
     1561 tgtattttga cacctctaat tatgtgtgta tacgtacaca tataattcgg tattttctta
     1621 acatatatat ccctcataaa aatttcttac atgcattgtt cgtgagtgac ccgttaatat
     1681 atatattgat agatactctt ataaaattat attctaaatt tcagattaag ctggcacaac
     1741 tatatttcca acatcactag ctaccatcaa aagattgact tctcatctta ctcgattgaa
     1801 accaaattaa catagggttt ttatttaaat aaaagtttaa ccttcttttt aaaaaattgt
     1861 tcatagtgtc atgtcagaac aagagctaca aatcacacat agcatgcata agcggagcta
     1921 tgatgagtgg tattgttttg ttcgtcactt gtcactcttt tccaacacat aatcccgaca
     1981 acaacgtaag agcatctctc tctctccaca cacactcatg catgcatgca ttcttacacg
     2041 tgattgccat gcaaatctcc tttctcacct ataaatacaa accaaccctt cactacactc
     2101 ttcactcaaa ccaaaacaag aaaacataca caaatagcaa aaatgtgaga ccacgaagtg
     2161 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     2221 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2281 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2341 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
     2401 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     2461 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     2521 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     2581 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     2641 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     2701 gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta
     2761 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     2821 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     2881 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     2941 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     3001 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     3061 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     3121 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     3181 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     3241 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     3301 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag
     3361 atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt
     3421 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     3481 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3541 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3601 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3661 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3721 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3781 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3841 tagttaccgg ataagg
//
