LOCUS       sequence-206370-        4238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pTEI068
FEATURES             Location/Qualifiers
     source          1..4238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2563..2581)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2649..2753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2975..3766
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3937..4238,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagta
      541 cgtaaactct tgtaaacgga tcacacaaga gtacggaaac cttagttacc gacaactaaa
      601 agtatacaat gatcaaacgg acataagtgg atttgattgg caactagtaa agtcaaatag
      661 atatataaat tgaaataata aataaaatgg atacaaatta aaagaagttt gacccaaaaa
      721 agaagaagaa atggatacat aagagctaat aaaaagaagg tttaaactta aaaaacaact
      781 ggattctctt taacacaaaa aacttgagat gcattttgtt gacgctaatg tttgataaca
      841 ttgctcctta ggcgtgctat actaggggtg tgagccaaag cacaattagc cttacctaaa
      901 tgaattattg tgttgcttag gcatgttaaa ctgagagtat gagtgtaaac aaatatcagc
      961 aagttcgtcc caaaaatgat aaattaagcg atgaggtaaa gagattcctc taatttaaaa
     1021 accacgtagc gctaaacaat taatctaagt tttgaattat gatgcagata ttgctgatcg
     1081 agaattttac aactaggcaa ccaaagttaa gaaatagatt gagatttttg attaggttga
     1141 ggcacaatag ggtgtggtta tggtacattg ttttgtataa ggatcatgta tgcgtgggtg
     1201 gtatgggact atgagagaat aatacgcgaa catgatcaat aaatattgac aaggtggttg
     1261 aagaaaaaaa aagatgaaac tcgggttcat atagaaaaaa atccaaagct gtgaatgatg
     1321 catgcaaaat tcggattaac ccatttattt cttatgtata aaatgattag tgcgtgagat
     1381 aaaaaaatac tcatgataga tagactagat aaaaaaatac tcatgataaa tagactaaga
     1441 tttttattag gattttgtaa aaaaaaacct tcaaacttgt tcatttttgc aattttattt
     1501 ccatttccaa caaatgtaaa ttaatcctat gaagtataaa ataatacgca ttataacatt
     1561 tttgcctatt tttgctatta tcaattttat aaaatattga tatatagttt tttaaaccga
     1621 actcgaaaag aagagtttaa ccttaccttc aaaattttaa aaatataatc taatgtgtgt
     1681 gaccttataa acgtttataa tatacgtata aggatcatat tgcgtatcat tttagacttc
     1741 aaatgatgaa cctgcatttg ttggagatga aggataaaat tccaaaaata gattagttaa
     1801 agaatttttt tgcacaaagc ctattttttt ctttgatgta tgtaagctta tcaactgagg
     1861 ttggtcaaaa atggattgaa taagaacatg tcgaccataa accaaataat ttaggtttta
     1921 gatgttttaa cactttgcta gaagccgagt atatataaaa ggatatatga ctcaagtttt
     1981 aggaccgaat tttgtatagt tggtttggat ctccataatc tcagagcttg gtggtcaaga
     2041 ttgtgcctcg taacctcgtt atccaaaaat gaagtagaat gtgagtcgaa tatcgtgaat
     2101 tcggtaccct ctcgttagct catagtgcgt tgaacccttt atctcgaatt tgtcaaaaca
     2161 aattgatatt ttgtcgtgaa aaaaaaaagt tcagacgaaa aatagtggtc acaagtcttg
     2221 ttcgaattga tacccgaaaa aacatcttaa ccaacttaaa gtaaaaattt aaaatcggaa
     2281 atttaaatat taccctaaag ttttaaaagg cggacttttc aacgaaggtt tatgctaagg
     2341 ttaaaaaaaa tacagaaacg ctttctcaca tttccatttc aataaatata tatcagtcgc
     2401 cacccaaatt tcagaattgc ttctcttagt atttgctttc accaacccac tcgacggagt
     2461 ctccaacgcc ttcctcctca ttctccactc tcacaccacc aacttctgat tccagatctc
     2521 aacaatgtga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg atacgcctat
     2581 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2641 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2701 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct
     2761 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     2821 tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg catccaagca
     2881 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     2941 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
     3001 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     3061 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gcgatattga
     3121 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     3181 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     3241 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     3301 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     3361 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     3421 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     3481 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     3541 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     3601 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     3661 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     3721 tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt cagaccaagt
     3781 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3841 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4081 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4201 taccgggttg gactcaagac gatagttacc ggataagg
//
