LOCUS       sequence-206371-        3262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pTEI069
FEATURES             Location/Qualifiers
     source          1..3262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1587..1605)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1673..1777
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1999..2790
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2961..3262,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagag
      541 aatggtatgt tgagtaaaga gatgcgatta gctgttgctt gatgccacgc acacgtcctt
      601 gtcctcatga aatattacca atactactat ctatatatgg atactgacag actaactctt
      661 ttaactcctg ttttaccaaa atgtgtatac atattataga attgtctaaa aacaggtcag
      721 ctaggctgtt tagatgggct gctgggcatt cagagtgatg gatataagtt gtggccacat
      781 gccatcatat cacttgtcca ttctttatct tatttcgcta tcaacattta catttactac
      841 tctgtgccat tacttcaatt atttgatgtc tgtattagtt tcactttcac ggtacgtaat
      901 cacatttgta tccatagccc aaaaaaaaaa cacatttata tccatatagt ttagttgccc
      961 tccttgtagt tttagacccc aaaagtcatc aaccggccaa aatcctcaat agtggcctta
     1021 ccaaacagag catatatgag tatcattaat tattgggact atctagggtt ttgattgttt
     1081 tcaaatacaa aattacatgc cttcttagca tattattttc acattattaa acataatttc
     1141 ttgtaaccct aaggaaggaa ccgcacatcc ataatgactt gactgacttt gtgtgtatgt
     1201 agctatagcg gcattttgga tcatcaccgg accaatcgac atcacatgtt ccttagtatt
     1261 gattcctgtt ttgaagttaa aaaagatatt acggtatgtg ctggatatgc gtggtctagt
     1321 gttgcaaaaa aaaaggaaga agattcaacg gccaccaaat ttcatcattt tagccaacaa
     1381 tgtatgttaa atgcgatcaa taaattcatg cagctgccat ctgttttcct ctctctcttc
     1441 attaacttaa tttctctctc tcacgcattt tgtctttgca acatcaccac catacataca
     1501 tacaacttac atacatacac gtcttgcaca agactcaaac tccacagaat gtgagaccac
     1561 gaagtggctc ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     1621 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     1681 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1741 ctgataaatg cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag
     1801 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     1861 ctgttttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     1921 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     1981 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     2041 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     2101 ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg
     2161 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     2221 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     2281 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     2341 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     2401 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     2461 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     2521 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     2581 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     2641 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     2701 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa
     2761 ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt
     2821 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2881 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2941 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3001 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3061 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3121 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3181 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3241 agacgatagt taccggataa gg
//
