LOCUS       sequence-206372-        3188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pTEI070
FEATURES             Location/Qualifiers
     source          1..3188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1513..1531)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1599..1703
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1925..2716
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2887..3188,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagga
      541 attgacgatg tagaattgta gatgataaat tttctcaaat atagatgggc ctaatgaagg
      601 gtgccgctta ttggatctga cccattttga ggacattaat attttcattg gttataagcc
      661 ttttaatcaa aattgtcatt aaattgatgt ctccctctcg ggtcattttc ctttctccct
      721 cacaattaat gtagacttta gcaatttgca cgctgtgctt tgtctttata tttagtaaca
      781 caaacatttt gacttgtctt gtagagtttt tctcttttat ttttctatcc aatatgaaaa
      841 ctaaaagtgt tctcgtatac atatattaaa attaaagaaa cctatgaaaa caccaataca
      901 aatgcgatat tgttttcagt tcgacgtttc atgtttgtta gaaaatttct aatgacgttt
      961 gtataaaata gacaattaaa cgccaaacac tacatctgtg ttttcgaaca atattgcgtc
     1021 tgcgtttcct tcatctatct ctctcagtgt cacaatgtct gaactaagag acagctgtaa
     1081 actatcatta agacataaac taccaaagta tcaagctaat gtaaaaatta ctctcatttc
     1141 cacgtaacaa attgagttag cttaagatat tagtgaaact aggtttgaat tttcttcttc
     1201 ttcttccatg catcctccga aaaaagggaa ccaatcaaaa ctgtttgcat atcaaactcc
     1261 aacactttac agcaaatgca atctataatc tgtgatttat ccaataaaaa cctgtgattt
     1321 atgtttggct ccagcgatga aagtctatgc atgtgatctc tatccaacat gagtaattgt
     1381 tcagaaaata aaaagtagct gaaatgtatc tatataaaga atcatccaca agtactattt
     1441 tcacacacta cttcaaaatc actactcaag aaaaatgtga gaccacgaag tggctcttca
     1501 gtggacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     1561 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1621 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1681 aataatattg aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1741 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg
     1801 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     1861 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     1921 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     1981 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     2041 tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga
     2101 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     2161 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     2221 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     2281 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     2341 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     2401 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     2461 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     2521 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     2581 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     2641 ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa
     2701 ggtagtcggc aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2761 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2821 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2881 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2941 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3001 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3061 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3121 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3181 ggataagg
//
