LOCUS       sequence-206377-        4324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pTEI73
FEATURES             Location/Qualifiers
     source          1..4324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2649..2667)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2735..2839
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3061..3852
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4023..4324,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagca
      541 cgttgtatcc atgaagctaa aattcatgaa ccaaaattag caaaggatgt ccacaacact
      601 gactgtcatt atatgatgtg tgtccacata tccaacgttt tatggtagag tttatatagt
      661 atagcatgtg aatgtattaa ccttatggat gtaattgtat ggttaatttt gttcatttaa
      721 taagattaac cagattaatt ggtcatggtt tccaaaatca tctcaaatcc tttgtaaaaa
      781 aaaaactaat ctgcgaaata taagtgaaga gaatcagagc tagatccacg tttgagagga
      841 taacgtcctc ttgacggaga tatgattgtt ggagcggcga agaaataggg tttagaactt
      901 cgagagaaag agagaaacgt atcttgccca agtggttaca tttttgatgc ggttgttgaa
      961 atttgggaga ggcttcacga tcatggaatc aaaatcgaaa gagagaatgt cgatagcgtt
     1021 gttgtaattt tcgagaaaac ttctgacgga aattctgtga ccagagactt tcacatgttt
     1081 tggaatcaga tccaacggag aaaggtggat ttgagaatat atttgaaaaa gatctcgaga
     1141 gaggaagaag aagagaatca atagattgtt gagagatagg gaagatgatg ataaacctta
     1201 agagattcaa ttttgatttt gaatattcag aatgtcctaa aagattgaat ttttattctg
     1261 gaaagagaga tgaagaagaa gataggccaa aaatgttttt gtattgtgaa agaagagaga
     1321 taacgaagag atattactaa ggaaaagaag agatagttag agagtagtga tgttaattca
     1381 catcaaaaaa gtgtctaatg attgtaaagt gcttaattac ggctaaattt tcagaaaagt
     1441 gcctcaacac gtaatattct ctagaaaaga tccattacat ctacacacaa cagtatagtt
     1501 taatttcttg gatttttttt ctgacaattt tattaaaatt tgaataccaa tgtcgatgta
     1561 gaactacagt tgtccacata tgaagttgaa tccataatct aaaatctaaa tttatataat
     1621 taaaaaaaaa aggttaacta acaagccaca gaagaaaaac acgatctttt gatgaaggta
     1681 aatttagaaa ctaatctcca acaaaatcca tttcaatgag ctatatatca actcacaagt
     1741 cgtaacccgt gccatcatta atattgttca cgtacatttt cgttttggca taagagtatg
     1801 gtttgagtta tatttcgtga aagtacatat tttaaaaaaa ctacttcttt gtttgtgtaa
     1861 gtgattggaa acgacatgtt tgatctcttt ttgttgttat tgaaaatgat tttaattgaa
     1921 aatcgaaatt cgtgttagga ctttacatat tttgttccct ttttagccta acacaataac
     1981 ctattcttgg tccgtccata actaaattct cagttctcga tcgcctacat atactttttt
     2041 tttttttttt tttgttagat tgatgtacgt aacttaaagt atatgtacct actcattcct
     2101 taatctagga ggacaacata tatgactaag atctagttct tggaactaca tgtttaacaa
     2161 cattagtact aattgggtga cacatgcata taaatataca aatggttata tctaaggata
     2221 ataattggat ctctaatttt caaactaata gcattatgga acgtatgtta gtgaaaaaag
     2281 tactctttaa tttattatta acatcaatat tagtcgccat tttgtaaagt catacgacac
     2341 ataatttttt aaattaccat cgaacctaaa agacattctc gtagttcaac gaacgcaaac
     2401 acacgtattc acttgttttc ttctggccag ctatatagtc tacgcatcgc atacgcaaac
     2461 catatataga tccatcactc tatatttaca tatatcaatg tatacacaga gagctatata
     2521 tagagaatac acaatacatt ctattcttgt atactcttat tacaaaaaaa aaaggagaga
     2581 gagagagata accaatttct ctccctgaaa atgtgagacc acgaagtggc tcttcagtgg
     2641 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     2701 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2761 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2821 atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     2881 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttttggggta
     2941 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     3001 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     3061 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     3121 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     3181 ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     3241 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     3301 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     3361 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     3421 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     3481 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     3541 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     3601 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     3661 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     3721 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     3781 tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta
     3841 gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3901 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3961 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4021 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4081 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4141 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     4201 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4261 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4321 aagg
//
