LOCUS       sequence-206378-        4619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pCK020
FEATURES             Location/Qualifiers
     source          1..4619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2944..2962)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3030..3134
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3356..4147
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4318..4619,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagta
      541 aggctagaca acgtccatag gtgtttatag aaagaaaaaa aaccttccat aggtttccat
      601 tgcattaaca caaacgggtt gattgattgc tctctcgcta atttttttta atactaatag
      661 tcatcataaa cttaaaatta gaaaccaagg ttgctttttg aggttcccaa aacttggaac
      721 ccggacttgt gtaaaaagaa agaaaaaaac aacccaaaaa actatgcaaa gtagtgaatg
      781 ttgtataaaa gcatatatca caacaacaaa aaagaatatt gcaaaaaaac aaagtatatg
      841 atatgtttgg gggcatccaa tacgagagtt ttgatatctc aaatccacga catgctatct
      901 ttaacttttt aatgtacaac tcacaactgt tttatcatcc agtaccagta attgatgact
      961 aattgggtgc tgggtgcatg ccactttttt ctatatatct aacaaatcta agtcataaag
     1021 tttctctaaa ccgaattaaa tccaaaaatc tacttattta gttagactcg agagtaggta
     1081 atgagatatt ctgtaagaaa tttgtaatta aacaaagatg ttatgtaaat taactgggat
     1141 gattccaagt aacataagcc acgtgaactt tgactttgac accgaattga tcaattttaa
     1201 aaacaagatg cagtttatat gcgagagaga caaagatatt tgtaagtact gttatgttgt
     1261 ctagtgaaga gtactttgtt tataaaaaca atgtttggtt ttattcacat tgtcatttta
     1321 ttatttggac cattttcgtc ttgcttagca tcccgtgcat gtctcatcta aatcctcact
     1381 attaagacat tttaacgcac actacatgaa tattataagt ttttcctttc tatttctgct
     1441 ggtgtcatac gtactgttca tatagacatc aataagtttc ttgtacaaac aactagacta
     1501 tgcaaaatta taagagctgg attgtatatt gttaaaactg aggcatgtat gagttgtacg
     1561 cttaaaagtg aaatattttt tcggtcgcag gttatatact agaatgcagt tagattttaa
     1621 ctaagaaata aatttttgac caaagcattt acagtataat gcatgattta tgttactatt
     1681 gctgctgtct cattttcaga tttcaaaaat cagatctaat tgacgtgata gcatatagag
     1741 gctagtaaaa cactatgagt ttatactttt attgatggtt aaaattattt ttacatcttg
     1801 cagtcatctt tcaaccattc ttcattctag aaggagttct tggtgactta ataaatggct
     1861 ttttacaaat attttggtgt aggacttata ttatacatgt gtgtggcgaa ccttctggtt
     1921 ctatctatta aatgcctttt ctttttgtag ttttttatga ttcagaattc agatatttga
     1981 ttttgtaata ttatattttc ttataaaaga gaataaaatt tcaaatttcc tgtctacact
     2041 tgccgaccaa cgttcctcaa gtgtcacatc ttctgtgcaa gagagacaaa actacaatct
     2101 gttcacacaa tgtcatgatg tttataatta atcttatcat cttcattcat atcaaactaa
     2161 tatcggccat ccatccgtct caataatgta attgcataca atctttgttt aggacttgct
     2221 agctatttca acttattatt gattacagat tagatctaca atttgtaaag aacttctaac
     2281 ttcaaaacaa atacaaaaaa aagaaagtga atgtaaaatt tctagtgtca taatttggaa
     2341 tagttgaagc attgaagtta caatatcgaa cgttaaaata ttgacactag atagtacatt
     2401 accaccaagg ctttctgatt attcttatca tgttatatga atagtaagaa attacgtaga
     2461 acgcaattta agtgtgtgcg agtcaccaca aattaaagga gaacaaattc aatgtttcaa
     2521 tcgctggttc cgatatacaa cttatgcatg ccatggcatg aatgcgagat ccaattatat
     2581 actaatatat acacacaaaa acatatgtaa atatagatgg aacagaagcc tagataggtt
     2641 aggataaaga aaacgatcaa atctgcaaag atcagtctct cccaaatccc caaaaaaaac
     2701 aaagcatgca tttcgtaata aacaactcat cataaaacga cacataactc gaaaacctct
     2761 cctcccgaca tttcatcaat tcatttctct ttttattttc gaaaagatga aaacttaata
     2821 aattattatg aacaatctta ctatatatat ggatatatac acaggcccta aaacgtaaaa
     2881 cagttgagga ctttacatct gaacaatgtg agaccacgaa gtggctcttc agtggacgaa
     2941 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     3001 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3061 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3121 gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     3181 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttttg gggtacagtc
     3241 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     3301 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
     3361 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     3421 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     3481 gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     3541 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     3601 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     3661 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3721 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3781 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3841 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     3901 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     3961 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     4021 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     4081 cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg
     4141 caaataactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4201 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4261 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4321 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4381 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4441 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4501 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4561 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataagg
//
