LOCUS       sequence-206379-        5564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pCK021
FEATURES             Location/Qualifiers
     source          1..5564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3889..3907)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3975..4079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4301..5092
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(5263..5564,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagtc
      541 gttgaagatg cctggattta atactgatta tataaagtta gggttgagat gacataacag
      601 aaataaatta tgggttcaaa agtataaaca cataaacaat aataataggt ttattcatct
      661 ttgggcttcc taaattaaat gggtcgtcat aaaaagggta atacttttaa ggcccattta
      721 atatactcat gaaacgacgc cgtgcacaag attggtctag ttggcatcta cctacctttt
      781 tggtattcat cctcaatatg caatttatcc atcaacaatg aacacttgta attaatttaa
      841 atgaaatctc ttagattgtt tggctaatat aaattttcat aaaatgtttt atataataaa
      901 tacccgaaaa atggtagtat ttaatttccc tccttgttat aaaatagcca ggtgattcag
      961 taattcacct gaaccaaacg gtgtacaaca catatatata tattgtatac taaaggtaat
     1021 tgcgtatcac tttgaatttt atacgtacac ttaattagcc ataatgggga ttctaaaata
     1081 agattataag tttcagaaca tgttggtatc tctatatgat taaacgctca ttaataaggg
     1141 gtttaatgca tgacgtgttc gtgaaggcaa cggcacatct caataagata atattgggcc
     1201 aacagaaact tgtaagtttc ataaattgtt ttacgaagag attatttacc gtgagaatcc
     1261 aatgttttta tgtgtttcgt gtatcttaca tggaatttag gaaattaaaa gttaccattc
     1321 atatcatatt cgtttttatc tttaatagtt ttatgtaatt cgtttgggga aaaaagtgag
     1381 aagaagataa attttgaggc gaatctcaag aagagagagt gaagtcaaac gagggacctt
     1441 gaattgtgga tttggtgttg gaccatcacg tgctctcctc cacggccttt caggattttg
     1501 cttcttctac cgcacgtgcc acactcctcc tcctacgtac cggtttccat tattgctttt
     1561 ttcttctttg cattccaatt cttcttctta ttctttttgt caaaggctct aaagtctcta
     1621 tatctatttg ttcgtttagt gaactttttt atttatatca aatggattat ttcataaact
     1681 tgtaagagtt atatttcagg aaatcatatc cacagttttg aagatcgatc ccccatgcct
     1741 ttttatatat acaaattcag ttatgattgt ttgacgcaac tgcagataaa tcatgtgtat
     1801 acacattata gggaaacatt aatagtaatc ctgctatttg cagaacctta agaacgtctg
     1861 tgaactgaac gtacgttatt atgtattaat atttcaactt taaagtttaa actaaaaaaa
     1921 aattagcaga agaaaattac atgaaaattg tcttacaaaa tgttaagaaa aaaaaacgct
     1981 agtggaaatg cgccaatatt acttgcacgt tcaatcatat agtataatta gctgttcatt
     2041 taaatcccat agtttctcta acgttttttt tttttttgat gaacactcta acgttattca
     2101 tataactaat atggtaagta aggagcaaat atggaacttt aaggattaag atcatttgta
     2161 gaaaatctta tttctaaatt tccaacgaaa ctgcactgaa attgatgcat taaaaaagta
     2221 agtgggagca taattcaagg tacgatacat catttttctt cccaacattt gactgcaatt
     2281 ttgagggcat agaagcttca attcctgttt tgttttgtcc ttgggataca taaaccagat
     2341 cttgtattat attatattta ccatgtacta tatagtattc gaagttcatt ctcattaatc
     2401 gattcctact tgttttcaaa gcaaattagt cagccttact atataacagc tgtaatccat
     2461 agtgattact gattagtata gttaatcctc ataatcatac ttacgtcaca gtagaaaata
     2521 ataatattgt agctatctaa tacgtaatat atacacaatt ctcttcttct ttttttttgt
     2581 caaagatagt atacacaatt ctttagatgt atatctaggt atttatactc gatgatttgt
     2641 acaaatatgt aaatcgaaga acttgatatt aattagaaac aaaaaacgaa acatagtgtc
     2701 gaagaaaaag attattttcg gcacaacatc atgcacaatc ttcattcgct atagaccttc
     2761 taagccctag ctaggcacaa cattggacat ggaacggtga atcttcaaaa ttatttgcag
     2821 atcaccatgt aagagagata catatatata gtctagatag gaatgatctg atcatcttag
     2881 ctgatgttta taccaatatg aataggttta tatatgtggc aagaaaaatt atttaatgga
     2941 aactgcctat acatattaaa gtatttctca tatatttctg tgaccagctt tggtttatta
     3001 aatttaatac aaaataatag tggcactgat ccatagaagc caagcttctc tatctttctc
     3061 tttccacact ttctttctat ctcaaagaag aagaaagaga gagagagaga gatagaaacc
     3121 ctaaacatcg tgcccttgaa gctaagatat cttcagcttt gtagaaattc attttgctta
     3181 cttcattgcg ctctctctct ttcattgtct actacctctt gcctctaaca gaagcaatac
     3241 tttccggcct ccaaggttcg tttctttcct catccatctt atatctttca ttgtcgtcat
     3301 catcatctgc atctacatat atatacacac acgcactccc tcacacttcc tcatatatat
     3361 catgtgtact tttcgtctta aacatctctc tattttcatt tcttttcttt gattacttaa
     3421 gaacatgact aagtctctat cattaaacct ctcttgttta agaagcagac aaagatcgtt
     3481 cagtttgcag caccttcatg tttctcttgg acattcaggt tttacttatg tgggtatatg
     3541 taatagaaga ttcatctttt gttttaaaat taaaggttaa taagttggag cctttgttct
     3601 tcgtgactat atgcatgcat ttggtaaacc tagggttttg atttttagat ctaatataac
     3661 ccaaacccca attaacacct tgagattatt ggatacatac ttgcatggtc aaacccattc
     3721 atttctcaat ttgttaaaac ttaaaacctt caatataatg tgtgtttctt tctttggggt
     3781 taattaatat ttcattaggg caaaggtcga aaagaaagcg tcaaagtttg cttaaagaag
     3841 agtaaggata atgtgagacc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac
     3901 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3961 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4021 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4081 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     4141 cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc
     4201 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     4261 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     4321 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     4381 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga
     4441 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     4501 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     4561 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     4621 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     4681 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     4741 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     4801 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     4861 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     4921 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     4981 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     5041 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
     5101 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5161 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5221 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5281 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5341 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5401 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5461 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5521 gtgtcttacc gggttggact caagacgata gttaccggat aagg
//
