LOCUS       sequence-206380-        5303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pAGM1761
FEATURES             Location/Qualifiers
     source          1..5303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             341..1132
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1303..1891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1792..1811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2045..2062
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5232..5250)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
       61 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      121 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
      181 cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc
      241 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
      301 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
      361 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
      421 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga
      481 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
      541 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
      601 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
      661 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
      721 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
      781 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
      841 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
      901 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
      961 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     1021 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     1081 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
     1141 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1201 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1261 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1321 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1381 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1441 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1501 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1561 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1621 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1681 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1741 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1801 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1861 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1921 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1981 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2041 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     2101 cgcgcgttgg ccgattcatt aatcactctg tggtctcagg agctatgaga ttactcgtgt
     2161 atcaattctc taattaatta aaaatagtat aaattaaata atatagttcg atacacgaat
     2221 ataattgcga agaataggca tacaaatttg tcatacatgt ttcgatatgg ctcacgagga
     2281 ggctgatgta acagtttgat gtatacgtat gcaaattgag aagtacttga tcagacctat
     2341 atatgtgatg ctcgaactta tctttttgtt ttggatcatc tatcgaatac aatggtacta
     2401 taatttaaat gttttttttc ttctttttct ttagtatcaa aagcaacgtt agatgctaaa
     2461 taaagagtta gttgattgtg atgactgata gtctgataaa atcattaact ttgcacccga
     2521 agtcaaataa aagtgttcat atttataaat tccaaccaac gttaataagc cacacctaat
     2581 cggtgattgc caacaatatt ataataaaat taaaaaaact acgactaaag ttaatttgct
     2641 ataattttgt ggtatgtttt aaaaacaaag ttctttagtt ctaatatcat gaaaattcag
     2701 tgtactgtaa aatatgtaaa aaggttttag tacagttctt ttttgtatat aacggcaaag
     2761 ttcaatacat attttactat tgaatttttt ttataaaaaa ataacaattg ctaccaactt
     2821 tttgaagcat attgatcgca acttaattag aattcttctt tttttttctt ggaagattaa
     2881 taaaacctaa tttcaatgtg gaacaaataa atgtagaaat attgttatca caaactaata
     2941 tatgatattt tttaatattt tcatatatac ttttgagatt ctgatgatat aacagttttc
     3001 attaaaatac aaattgtcgt gtactaattt ttcttttgtt caagtatgtg ataaaaatat
     3061 gttgcaaatt gcgagttatt ataatgttac aaatatgtag agagaataca tgagaagagt
     3121 taaaagaagc atgcttaagc caacagagag tggatccaaa tgttgctttc cagctttata
     3181 caaacgtatc acccacatta ctgccactgc tacatatatt gaaggagaga gagatgatga
     3241 tcataatgat aaatcgatgt cgatgataaa ttgatgatga tggctccggt atgtgtaccc
     3301 taggagttgt agctagctag ctaggaccat gtatatacat acatacatat attagtgttt
     3361 tttgtaactt gtacgtacct tacatagagt atggagttta ctaaaacggc aacgtttggt
     3421 gggggtagtg aattcgcaag tggggatgag tctatgtaat agaagatgca acatgcaaat
     3481 ggtccccttt ctgtttttat ttaaagaaat gagtgtttac tgaggaggaa acatcccatt
     3541 tatagattca cacccataaa agcaaaccac ttctccttct ttttatttcc ccatgatatt
     3601 acttcgagaa tattttgaaa atttgaagtg tacatttaga gattgtgtac tttgaacact
     3661 catgtcaaat gcatctaaat acataaactc caatttaaaa taatcgtcta aacctagagt
     3721 gccatttgtt tagccatttg ttggtcatca tttctcatgc tttgattaca tgtaccggtt
     3781 gattcatgtg aaaaatcatg tgcataaact aagaaatagc tagcacataa aattttgatt
     3841 taggttggat attactatgt tcactttaag agaaaaaaaa cttatggcaa aaagtgatga
     3901 tggtatatga atatgataat caaagtgcat atgtgaagtg agaggcaact gtagagtaat
     3961 ataataaaat ccaaagaaaa tttttaaata tgagaaaaaa ttatataaaa aggttctttt
     4021 gtaatccact tcttttgata tagggagatt cgttgagcat ccatgtgctc tttcaatcga
     4081 cactattctg tctgtatcta gccaaccaca tataccttta cactagagaa cttcgatgat
     4141 tctttttcaa aatcaatgtg atataatata attaagcata tatgcataaa aaatgaagaa
     4201 gaatggtaga gtcatgttac ttaaggtcat ggtgtgtaaa aacattgata ctttacaata
     4261 tatgagttgt gaagtgctct taaagttata acatccggtt ctacgtattg acctagaact
     4321 agaagaatcg ttttttagtc caaatcaaat caagtcggtt ctttatcagt tttgttgtat
     4381 gtgaattaat ttgaaaatat tagctatgat cttagcttgg gtttttgttt ctaagggtta
     4441 aggatcatat ctctttgtca aatgacatgt ggtctatatg tcatgaatta ggcaccgcta
     4501 tcttttacta ttgattcgac gacattggga ctcctcacta cacttatctt aaaaaaactc
     4561 aaagttggtg ttaatggctt gtcaccataa actttcatga gctctaacaa attaaacttg
     4621 aacttgatca ggtgtcacaa tatatacaat ttcgagggat aaatatttca aaaggataat
     4681 atgatagttg gtagaaatgt atagtttcta gtaataatag agatcgttgg ttaaactccc
     4741 caacttttta aaattaattt gattagtgga tccgcaaaca aatattagat tgggcctata
     4801 tgcatctata ttatttttat ttttctgtaa tttcagtaaa atgggcctat ggtcctatat
     4861 gcatccgaat aattagtata ctgggcttat gggcctatat gcatttgatt ttatcgataa
     4921 aatgtgagtc aaatgtctaa tgtgcgccgt tatgaagtgc aagtggctaa tttttttcac
     4981 ctagattcct tctattgacc gtcgatagac ggatgataac tatgacgtgg cattatcgca
     5041 gccatcaaac aaagtcatgt ataacaaaca agagcacaca aacgaaaaca aattcagttg
     5101 cggaacccaa attcaaatca acggaattag aatcacgctt tcaattccgt aacccgccat
     5161 taaaaacctt gaaccctcga agcaaatcga gctacttgag accacgaagt ggctcttcag
     5221 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5281 ttcttagacg tcaggtggca ctt
//
