LOCUS       sequence-206381-        3221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG020
FEATURES             Location/Qualifiers
     source          1..3221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1546..1564)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1632..1736
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1958..2749
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2920..3221,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagat
      541 tacggatatg aatataggca tatccgtatc cgaattatcc gtttgacagc tagcaacgat
      601 tgtacaattg cttctttaaa aaaggaagaa agaaagaaag aaaagaatca acatcagcgt
      661 taacaaacgg ccccgttacg gcccaaacgg tcatatagag taacggcgtt aagcgttgaa
      721 agactcctat cgaaatacgt aaccgcaaac gtgtcatagt cagatcccct cttccttcac
      781 cgcctcaaac acaaaaataa tcttctacag cctatatata caaccccccc ttctatctct
      841 cctttctcac aattcatcat ctttctttct ctacccccaa ttttaagaaa tcctctcttc
      901 tcctcttcat tttcaaggta aatctctctc tctctctctc tctctgttat tccttgtttt
      961 aattaggtat gtattattgc tagtttgtta atctgcttat cttatgtatg ccttatgtga
     1021 atatctttat cttgttcatc tcatccgttt agaagctata aatttgttga tttgactgtg
     1081 tatctacacg tggttatgtt tatatctaat cagatatgaa tttcttcata ttgttgcgtt
     1141 tgtgtgtacc aatccgaaat cgttgatttt tttcatttaa tcgtgtagct aattgtacgt
     1201 atacatatgg atctacgtat caattgttca tctgtttgtg tttgtatgta tacagatctg
     1261 aaaacatcac ttctctcatc tgattgtgtt gttacataca tagatataga tctgttatat
     1321 catttttttt attaattgtg tatatatata tgtgcataga tctggattac atgattgtga
     1381 ttatttacat gattttgtta tttacgtatg tatatatgta gatctggact ttttggagtt
     1441 gttgacttga ttgtatttgt gtgtgtatat gtgtgttctg atcttgatat gttatgtatg
     1501 tgcagcaatg tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc
     1561 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1621 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1681 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc
     1741 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     1801 ttttcatggc ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa
     1861 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1921 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     1981 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     2041 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat
     2101 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     2161 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     2221 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     2281 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     2341 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     2401 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     2461 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     2521 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     2581 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     2641 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2701 atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca
     2761 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2821 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2881 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2941 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3001 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3061 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3121 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3181 tcttaccggg ttggactcaa gacgatagtt accggataag g
//
