LOCUS       sequence-206382-        3220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG575
FEATURES             Location/Qualifiers
     source          1..3220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     455..472
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1559..1577)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1645..1749
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1971..2762
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2933..3220,1..301)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
       61 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      481 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactctg
      541 tggtctcagg agattacgga tatgaatata ggcatatccg tatccgaatt atccgtttga
      601 cagctagcaa cgattgtaca attgcttctt taaaaaagga agaaagaaag aaagaaaaga
      661 atcaacatca gcgttaacaa acggccccgt tacggcccaa acggtcatat agagtaacgg
      721 cgttaagcgt tgaaagactc ctatcgaaat acgtaaccgc aaacgtgtca tagtcagatc
      781 ccctcttcct tcaccgcctc aaacacaaaa ataatcttct acagcctata tatacaaccc
      841 ccccttctat ctctcctttc tcacaattca tcatctttct ttctctaccc ccaattttaa
      901 gaaatcctct cttctcctct tcattttcaa ggtaaatctc tctctctctc tctctctctg
      961 ttattccttg ttttaattag gtatgtatta ttgctagttt gttaatctgc ttatcttatg
     1021 tatgccttat gtgaatatct ttatcttgtt catctcatcc gtttagaagc tataaatttg
     1081 ttgatttgac tgtgtatcta cacgtggtta tgtttatatc taatcagata tgaatttctt
     1141 catattgttg cgtttgtgtg taccaatccg aaatcgttga tttttttcat ttaatcgtgt
     1201 agctaattgt acgtatacat atggatctac gtatcaattg ttcatctgtt tgtgtttgta
     1261 tgtatacaga tctgaaaaca tcacttctct catctgattg tgttgttaca tacatagata
     1321 tagatctgtt atatcatttt ttttattaat tgtgtatata tatatgtgca tagatctgga
     1381 ttacatgatt gtgattattt acatgatttt gttatttacg tatgtatata tgtagatctg
     1441 gactttttgg agttgttgac ttgattgtat ttgtgtgtgt atatgtgtgt tctgatcttg
     1501 atatgttatg tatgtgcagc cattgagacc acgaagtggc tcttcagtgg acgaaagggc
     1561 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     1621 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1681 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1741 aggaagagta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     1801 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc
     1861 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     1921 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag
     1981 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2041 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2101 cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2161 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     2221 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2281 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2341 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     2401 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     2461 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     2521 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     2581 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     2641 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     2701 atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat
     2761 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2821 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2881 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2941 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3001 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3061 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3121 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3181 gcgataagtc gtgtcttacc gggttggact caagacgata
//
