LOCUS       sequence-206386-        3264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG535
FEATURES             Location/Qualifiers
     source          1..3264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1589..1607)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1675..1779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2001..2792
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2963..3264,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagca
      541 gcattaaggc acatcagaga ttttttgggt gttaagtttg tcatgaaacc tgatgcctcc
      601 acaggaactg tcaatctcat gtgtcttggc tctggttttc agaatttatc cagaaaagta
      661 tcatgataaa ttaatggtgt ctgtgtttgg tggcttagag tgacggctag atcaacatct
      721 ttgggatgcc ttgtggagtg aaatcaagca tactttatca taggcgaaat tttttgttgt
      781 ggtttgctgc ttgtaatgag agagtgatat aggaagcaaa tgtggagatc acatttgctc
      841 atctccttgt tgcgttgaaa cttttggtgt caagagttct aattcacatg tatttgaaga
      901 ttcctcatat gctgcttttg tttctaatta ttttttctag taagaaaaca tttgttcctg
      961 agtttccaac tagaaaaaaa tatcaagtaa aatagaattc aatcatttcc cttaccaacg
     1021 cttggtactg ccaaccgcaa caaagaatta atgcaaaaca acagtctatt aatatcaacc
     1081 tagactaaac tccttagttt tactttgaaa tgcgaatgat acatgacaca ttagattgta
     1141 cttgcttttt accacagata caacgataca tttgtatatc ttttccctta tagcaaactc
     1201 taatatatca tagtcaagct aacgaaactt atgcaaggga aatatgaaat tagtatgcaa
     1261 gtaaactcaa agaactaatc attgaactga aagatcaata tatcaaaaaa aaaaaaaaac
     1321 aataaaaccg tttaaccgat agattaacca tttctggttc agtttatggg ttaaaccaca
     1381 atttgcacac cctggttaaa caatgaacac gtttgcctga ccaattttat tatataaacc
     1441 taaccatcct cacaacttca agttatcatc ccctttctct tttctcctct tgttcttgtc
     1501 acccgctaaa tctatcaaaa cacaagtagt cctagttgca catatattta atgtgagacc
     1561 acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat
     1621 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1681 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1741 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc
     1801 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     1861 gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     1921 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     1981 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2041 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2101 acggctccgc agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg
     2161 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2221 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2281 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2341 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2401 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2461 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2521 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2581 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2641 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2701 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtaa
     2761 aaggcgagat caccaaggta gtcggcaaat aactgtcaga ccaagtttac tcatatatac
     2821 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2881 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2941 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3001 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3061 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3121 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3181 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3241 caagacgata gttaccggat aagg
//
