LOCUS       sequence-206387-        3266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG577
FEATURES             Location/Qualifiers
     source          1..3266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     455..472
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1605..1623)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1691..1795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2017..2808
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2979..3266,1..301)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
       61 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      481 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactctg
      541 tggtctcagg agcagcatta aggcacatca gagatttttt gggtgttaag tttgtcatga
      601 aacctgatgc ctccacagga actgtcaatc tcatgtgtct tggctctggt tttcagaatt
      661 tatccagaaa agtatcatga taaattaatg gtgtctgtgt ttggtggctt agagtgacgg
      721 ctagatcaac atctttggga tgccttgtgg agtgaaatca agcatacttt atcataggcg
      781 aaattttttg ttgtggtttg ctgcttgtaa tgagagagtg atataggaag caaatgtgga
      841 gatcacattt gctcatctcc ttgttgcgtt gaaacttttg gtgtcaagag ttctaattca
      901 catgtatttg aagattcctc atatgctgct tttgtttcta attatttttt ctagtaagaa
      961 aacatttgtt cctgagtttc caactagaaa aaaatatcaa gtaaaataga attcaatcat
     1021 ttcccttacc aacgcttggt actgccaacc gcaacaaaga attaatgcaa aacaacagtc
     1081 tattaatatc aacctagact aaactcctta gttttacttt gaaatgcgaa tgatacatga
     1141 cacattagat tgtacttgct ttttaccaca gatacaacga tacatttgta tatcttttcc
     1201 cttatagcaa actctaatat atcatagtca agctaacgaa acttatgcaa gggaaatatg
     1261 aaattagtat gcaagtaaac tcaaagaact aatcattgaa ctgaaagatc aatatatcaa
     1321 aaaaaaaaaa aaaacaataa aaccgtttaa ccgatagatt aaccatttct ggttcagttt
     1381 atgggttaaa ccacaatttg cacaccctgg ttaaacaatg aacacgtttg cctgaccaat
     1441 tttattatat aaacctaacc atcctcacaa cttcaagtta tcatcccctt tctcttttct
     1501 cctcttgttc ttgtcacccg ctaaatctat caaaacacaa gtagtcctag ttgcacatat
     1561 atttaccatt gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct
     1621 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     1681 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1741 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg
     1801 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     1861 tttcatggct tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag
     1921 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     1981 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
     2041 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     2101 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt
     2161 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     2221 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     2281 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     2341 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     2401 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     2461 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     2521 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     2581 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     2641 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     2701 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     2761 tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa
     2821 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2881 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2941 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3001 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3061 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3121 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3181 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3241 cttaccgggt tggactcaag acgata
//
