LOCUS       sequence-206388-        1658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and 
            tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, 
            Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
  TITLE     FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's 
            Disease Modeling.
  JOURNAL   Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 
            10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
  PUBMED    28988985
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.08.026"; journalName: "Stem Cell Reports"; 
            date: "2017-11-14- 14"; volume: "9"; issue: "5"; pages: "1423-1431"
COMMENT     Sequence Label: pDONOR-SNCAe3-A53T
FEATURES             Location/Qualifiers
     source          1..1658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(6..797,798..866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     629..648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(867..971)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(997..1452)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1134..1155)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1346..1365
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1579..1601
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1593..1610
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1594..1610
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1620..1639
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1620..1638
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
       61 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
      121 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
      181 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
      241 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
      301 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
      361 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
      421 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
      481 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
      541 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
      601 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
      661 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
      721 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
      781 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
      841 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
      901 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
      961 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     1021 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1081 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     1141 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     1201 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     1261 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     1321 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     1381 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     1441 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     1501 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     1561 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     1621 aatacgactc actatagggc gaattggggc gcgccatt
//
