LOCUS       sequence-206390-        3456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-146
FEATURES             Location/Qualifiers
     source          1..3456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     31..48
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1371..1389)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1457..1561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1783..2574
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2745..3333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3234..3253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
       61 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt
      121 ctcaaatggc gggtatcaat agagcgggac catcgggcgc ttattttgtt ggccacacag
      181 accccgagcc agtatcgggg caagcacacg gatccggcag cggcgccagc tcctcgaaca
      241 gtccgcaggt tcagccgcga ccctcgaata ctcccccgtc gaacgcgccc gcaccgccgc
      301 caaccggacg tgagaggctt tcacgatcca cggcgctgtc gcgccaaacc agggagtggc
      361 tggagcaggg tatgcctaca gcggaggatg ccagcgtgcg tcgtaggcca caggtgactg
      421 ccgatgccgc aacgccgcgt gcagaggcaa gacgcacgcc ggaggcaact gccgatgcca
      481 gcgcaccgcg tagaggggcg gttgcacacg ccaacagtat cgttcagcaa ttggtcagtg
      541 agggcgctga tatttcgcat actcgtaaca tgctccgcaa tgcaatgaat ggcgacgcag
      601 tcgctttttc tcgagtagaa cagaacatat ttcgccagca tttcccgaac atgcccatgc
      661 atggaatcag ccgagattcg gaactcgcta tcgagctccg tggagcgctt cgtcgagcgg
      721 ttcaccaaca ggcggcgtca gcgccagtga ggtcgcccac gccaacaccg gccagccctg
      781 cggcatcatc atcgggcagc agtcagcgtt ctttatttgg acggtttgcc cgtttgatgg
      841 cgccaaacca gggacggtcg tcgaacactg ccgcctctca gacgccggtc gacaggagcc
      901 cgccacgcgt caaccaaaga cccatacgcg tcgacagggc tgcgatgcgt aatcgtggca
      961 atgacgaggc ggacgccgcg ctgcgggggt tagtacaaca gggggtcaat ttagagcacc
     1021 tgcgcacggc ccttgaaaga catgtaatgc agcgcctccc tatccccctc gatataggca
     1081 gcgcgttgca gaatgtggga attaacccaa gtatcgactt gggggaaagc cttgtgcaac
     1141 atcccctgct gaatttgaat gtagcgttga atcgcatgct ggggctgcgt cccagcgctg
     1201 aaagagcgcc tcgtccagcc gtccccgtgg ctcccgcgac cgcctccagg cgaccggatg
     1261 gtacgcgtgc aacacgattg cgggtgatgc cggagcggga ggattacgaa aataatgtgg
     1321 cttatggata agctttgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat
     1381 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1441 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1501 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1561 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     1621 ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca
     1681 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1741 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     1801 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1861 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc
     1921 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     1981 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2041 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2101 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2161 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     2221 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     2281 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     2341 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2401 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2461 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2521 gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca
     2581 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2641 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2701 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2761 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2821 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2881 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2941 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3001 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3061 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3121 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3181 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3241 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3301 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3361 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3421 gtggataacc gtattaccgc ctttgagtga gctgat
//
