LOCUS       sequence-206423-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression vector for mCarmine, a far red emitting 
            fluorescent protein (excitation: 603; emission: 675).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Fabritius A, Ng D, Kist AM, Erdogan M, Portugues R, Griesbeck O
  TITLE     Imaging-Based Screening Platform Assists Protein Engineering.
  JOURNAL   Cell Chem Biol. 2018 Dec 20;25(12):1554-1561.e8. doi: 
            10.1016/j.chembiol.2018.08.008. Epub 2018 Sep 13.
  PUBMED    30220597
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2018.08.008"; journalName: "Cell Chem Biol"; 
            date: "2018-12-20- 20"; volume: "25"; issue: "12"; pages: 
            "1554-1561.e8"
COMMENT     Sequence Label: mCarmine in pcDNA3
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..735
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein with 
                     favorable expression and pKa properties and with peak 
                     emission at 675 nm (Fabritius et al., 2018)"
                     /label=mCarmine
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLKVKQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTETLYPADGGLRGYNT
                     MALKLVGGGHLQTFLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 atggtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag
       61 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      121 ggcacccaga cccagagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
      181 atcctggcta ccagcttcat gtacggcagc aagaccttca tcaaccacac ccagggcatc
      241 cccgatttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
      301 gaagacgggg gcgtgctgaa ggtgaaacag gacaccagcc tccaggacgg ctgcctcatc
      361 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
      421 acactcggct gggaggccaa caccgagacc ctgtaccccg ctgacggcgg cctgcgcggc
      481 tataacacga tggccctgaa gctcgtgggc gggggccacc tgcagacgtt cctcaagacc
      541 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctttgtggac
      601 cgcagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg
      661 gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa tggcatggac
      721 gagctgtaca agtgataata g
//
