LOCUS       sequence-206429-       15213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to dysferlin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15213)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 15213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-DysF
FEATURES             Location/Qualifiers
     source          1..15213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5092..5116
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5117..5126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5123..5818
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(5820..5844)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6539..6550
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(12110..12130)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(12138..12154)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    12170..12304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12194..12213)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12248..12267
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(12743..12930,12931..12942)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(12976..12992)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12976..12992)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13029..13049)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13029..13047)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13068..13084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13081..13103)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13092..13108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13116..13122,13123..13140,13141..13146))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13161..13182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13299..13316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13470..14058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13550..13569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14229..15020,15021..15089))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14852..14871
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15090..15194)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggca cgcgcagatt ccgtgtgggc
     1141 ctggtaatat cttaccattt taagctattt taagattagg gaaaaaaatt aaataaaact
     1201 actttaaaat tactttcctt aaaaagcaac atatttattt agtttttgtt taactggctc
     1261 aagattgtaa tattttactt ttacatgttt tttttaagta cctaaatgtg ttcaaaagta
     1321 aagtatgtta ttgttttttt ttttttaatg ttagctaagc tttaaattgt aatatattac
     1381 ttttaaaagt gactttttaa aaatgtaaat aaaaaagtaa ctataaatgt gtaatgcatg
     1441 ttatttctgt gggtttttta attttcatct caactgggct tacaatttta acataataaa
     1501 aatcaaatca aatcaaatca ggcccttatt tgtcacatac actcttgtat actgaaataa
     1561 actaaatgtt gagaaaaatt taaatacacc aaaataacgc ataccttttt tgtaattacc
     1621 taagtaaaac atttagttcc ttttttaagt gactcaagat tgtaatctat taattgtaaa
     1681 agataaaact agtgatcaat ctgacgtgct tctatgcagc ctgtgagatt aattgtgatg
     1741 tttaattatt aaacaattta aatggatcaa acatttcaca tgtgcttatt gtgtccaaca
     1801 aaaccagcag aagcacttaa aaatattgca tgacaacatg agtttttaat gtaaattata
     1861 tatacttcct aaacgaagtt aatcatattt tctaaaataa taatgtcatt gaaatattta
     1921 aataaatgat aanctttagt tgattcaaca taaaattgaa agcaataagg actttttttt
     1981 ttttacagcg gtgaagcaaa agcntgnaaa taatcatata aatataataa ataaataaat
     2041 aaaataatgg ttcttggtgc aaatatgaac actttaaata aatatagaat aaatatgatc
     2101 aataagtcat aacgacgtgt tttatgttga ttataattta aacatgtttc taacgcaagt
     2161 tttaaaaact gttttaaagt attattttaa gttataagat caatttgact caacattttg
     2221 tagtgaaaat atgttgtgta aaattaatat aatatacact ataaaaatgc aaggttccac
     2281 acaattcatt catgttgtcc caacacaaat cgattaagtt aacaaatata tgtggattga
     2341 acataaaaaa ttaagttgtc ccaatgaaat ctcaagaatt gtgttgtttc agctcatttt
     2401 aaataagtag ttttaacaag taaaaaataa catatttttg agtgtgtata tatatatatt
     2461 tatatattac aaaatataat aatataaaat gtaaaagata taaaccatgc tacatatatt
     2521 taagagctat tttgcacatt acttttttgc aagccatttg agtgtttttt tacaaatata
     2581 tattagctaa atatacaata tcttaaatat acaattaatc agacatgcaa aaaatgcatt
     2641 taacctacat aaaaatacat gatcaacata tgttttatgc tgattacaac taaatcaaac
     2701 gtaatgaaat taatttataa aagcgattta aatatgtcaa tattttaagt taaaatcact
     2761 tgaaacctac tataaatgaa cttaaattta aaaaatctcc tttaattcca atgcagcact
     2821 gttgtttttt cagctgagga cccccggtgc agatggttgg cccccggccc gctattctaa
     2881 ggaagccttt cttaatttta gggcggcgct gcatgcgctc agatcagatt cacgggtgga
     2941 gagggttatt ttctcttttt tattttttct tgcctgctca tatttgggcc tgtgtgaacc
     3001 ccaggctccg cctcggcccc caggagccgc tcttatttaa aggggatcct cgccttcagt
     3061 cttcgtccca gtggaagcat cagtcttgtg ataacaactt ggtgagtcct acagaccagc
     3121 gctcgactct aggctttaaa tgcattgatt tctctggatt agctaccggg agagttttaa
     3181 ctttgcttat tagtcttgtg cttgaactgc taggtttaaa gagggttttc tctaatgcat
     3241 gcatgaagcg acagctgaaa accagaaata gagcgtttgt cctcctgtta aactctagcc
     3301 tttctttggc atttgtcagt ccgagtcttc agctgccggc aggagattgg gtttcagtaa
     3361 cacttgctga gcacaaacta cacgtgcttc cttcagattt aattcattat taatcattct
     3421 agatttaaat gatcttctta ctcattgctg attattttag ccttgcggag cagattaaat
     3481 gagcacgtgt ttattaagat tgatttaaat ataattaata tttcaccact gtagttttaa
     3541 aattgccttc taagtcatgg ttgattttta ataggtatcg gctctaatta gattactatt
     3601 ttggttttta aatgttattt ctatttcatt tgtaggctcg tttttaatgt gattttatta
     3661 gattctagtt ttaaaatcat cttttagcct tactgagcag actgaatgaa catgtttcat
     3721 taagattaac ttcattatag ttaattattt aatcattcta gtggattata aatcagctct
     3781 atttaaattg attgtcgtgt ttatttctat ttgatttttg attatttttt aaggtcgttt
     3841 ttatgtgatt tttccccgat tctaatttta aactaattgt ttgattttta tatgattcga
     3901 ctctttttaa atgaattgtt ttgctttttt tattgagcat atatatatat tatacaattt
     3961 caatcaacaa ttgttgtgct ttttaaatgt tgtttctatt tcatttctag ggtcgatttt
     4021 attcgattct agttttaaaa tcgtgatttc taactcctaa ccttactgag cagactgaat
     4081 gaacatgttt catcaagatt aacttcatta tagttaatta ttgaatcggg ctagtggttt
     4141 ataaatcagc tctattataa attaattgtc atcattattt ctatttaatt tttattattt
     4201 ttttaggatt atttttaagg gatttttttc acgttctagt tttaaactca atgctcatta
     4261 ctgctcaccc ttgctgagca gtctttcgtt aagattaaat taattaataa cccattctag
     4321 ttttaaaaca gtgtcttaag gcatagctga tattttacaa gtttactatt taatatatct
     4381 ccatttcttt ttttagggtc gatttcatct gatttgatta atttttatta tcttcactaa
     4441 gctttttgtt ctaggtttta ttctcagtat tattactgtt atatttgaac tgaatttctc
     4501 ctcatagttt attcatttta tttatttatt ttttgtttat tcgctatttt gtattttagt
     4561 ttttaattag aaattttatt agaatcgttt tgaactagtt tttatgcact ctaacattaa
     4621 tattaaatca taactattat ttttattata atttatttgt gtttatcttt ttgtgtagct
     4681 ttcatgtgtt tttattttag ttttattatg tttgcattga attttttgga ctagtttata
     4741 taacgatata cagaaaagcc atgacaacaa atgtttttat aatacctatt aagtataagc
     4801 taatcagatt tccactattc tgttcaaagt taacattatt agccattctg atggatcatt
     4861 tgacatttgt catatgtctt tatgaacact gctctttcag atgccacttt taaagctgtt
     4921 ttttacagta aatgaacgtt tattattatg acattatttc ctaaggcctc tagagtacag
     4981 caggatgtgg cgtgtgcatt actgtgtaat tatgctgact gtaaatgtgc atttatagac
     5041 tgtcagcatg tctgactgtg cgctgttgtg atttgtcctg cacagaccaa ggcaagtttg
     5101 tacaaaaaag caggctgcca ccatggtgag cgagctgatt aaggagaaca tgcacatgaa
     5161 gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg
     5221 caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg gccctctccc
     5281 cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct tcatcaacca
     5341 cacccagggc atccccgact tctttaagca gtccttcccc gagggcttca catgggagag
     5401 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     5461 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt
     5521 gatgcagaag aaaacactcg gctgggaggc ctccaccgag accctgtacc ccgctgacgg
     5581 cggcctggaa ggcagagccg acatggccct gaagctcgtg ggcgggggcc acctgatctg
     5641 caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt
     5701 ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga
     5761 gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacagaga
     5821 cccagctttc ttgtacaaag tggtgttgag gtgtttgcta cagagagcga ccaacctgag
     5881 aaatgtagag aagggggaaa ggcatagcga cccgcttgct agcatcactt tcagagggac
     5941 aaaaaagaag actaaagtca tcaaaaataa tgtcaatcct gtctggaatg agggctttga
     6001 gtgggacctg aagggtgttc ctttagattc gggagcagag cttcatgttg tcgttaaaga
     6061 ccatgagaaa atgggcagga ataggtttct gggggagtgt cgagttgctc ttcgagatgt
     6121 gctcaattct ccaaacctgg ctgctacttt taccgtgtcc ctggtcgaca caaagagaaa
     6181 cagcactggg gccacaataa ccctgcaggt ctcctacatc cctcctccag ggatgacgcc
     6241 catctttcag cctcctccac agccagaggc ccagcacaca ccggtggagc tggacacagt
     6301 cacagttttc tcattggaca ctatgggcga ggatgacact gagagcatgc tgatgatgga
     6361 ggtggcggag gagcctgaac ctgttccagg accccagggg caggacatcg gcgcccctac
     6421 agcaccaccc aaaaaggctc cacccaactt taaccaggga ctaaagaaag agaaacgcca
     6481 aagcaacaaa tcccccctgt ccgacaaagc ccaggacctc caggttcggc tgcgcatcat
     6541 tgaaggaagg cagcttcctg gtgtcaacat caaaccagtc gtcaaagtca cagttggagg
     6601 acagacgaag agaacccgca ttaaaaaggg caataatcca ttttttgatg agaccttttt
     6661 tctaaacttc tttgagaaac cttcagatct ttttgatgag ccaatattca taaccgtatt
     6721 tgattccaag tcactacgga aagactctgt cattggagaa ttcgagctgg atgttggcat
     6781 ggtttataat gaacaccgac atgctattct cagaaaatgg ttgttgctgg cagaccctga
     6841 tgatctttct gcaggggcca gaggttattt gaaggtcagc atgtttgtgc tggccgcagg
     6901 agatgaacct ccgatggata agaaagaggg tgtggaggaa aaggaggaca tagaggccaa
     6961 tctgttgaga ccagctggtc taaccctgag aggagccacg ttcactctga gagtctacag
     7021 agcagaagac cttcctcaga tgggtgatgc tcttattgac gatgtcaaac atttccttgg
     7081 ctttgacact aatcgaaaga acctggtgga tccattggtg gaggtcaact ttgcaggaaa
     7141 aacggtaagt agtaagattc tggagaaatg cgctaaccct cagtggaacc agtgcctcac
     7201 tctgcccatc aggtttccat ccatgtgtga aaagatgaga atacgtattt tagattggga
     7261 tcgtgcgagt cataatgatg tcattggcac tgcacactta tgcatgtcta aaatctcagc
     7321 ccctggagga gatattgaag tggatgacag tataggcttt cttccgacct ttggaccctg
     7381 ctttgtcaac atgtatggga gtcctcgaga gttcacagct ttctcagacc cacacgaggc
     7441 actaaacctg ggaaagggag agggtgtggc ctatcgggga cgtgtgttgt tggagctcat
     7501 aacaaagctt tctgatcagg tggaccaaag gtctgaagac atcaactctg atgacctgct
     7561 agtggtagaa aaattcatgc ggaagaggaa gttttccctg tttgctgcgt tctactcggc
     7621 cactctcctt caagatgtgg acgatgcagt tcagtttgaa gtcagtatcg gtaactacgg
     7681 caacaagttc gacaacacct gtttgcctct ggcatccacc actcaattca gcagagctgt
     7741 gttcgacggc tgccattact attacctgcc atggggaaat gtgaaacctg tggtggtggt
     7801 gtcttcagag tgggaagaca ttggccctcg tatagaggcc ctcaacatgc tgcttagcat
     7861 agtcgaccga ctggagtcaa acttggaaca ggtacagctg gagctcaaag caagaagtga
     7921 cggtcaggaa gtcgagcaga gggttgtcca gctgcttgat gatgtcattg cagactgcag
     7981 tgaggaactc cctgagttgg acaaatggcc ttgtgtgact ccactggata aagctctgag
     8041 gaatctgcgc aagattaaca tgcagcagat agtgcaggcg gccctggcac tcaaacatgg
     8101 accagagact gaactgagtg cagtgctgga gcaggctgag gactggacca tgagactacg
     8161 ctacctctct gaggagcccc aaaacagtct gccagatata ataatctgga tgcttcaggg
     8221 agacagacga gtggcctacc acagaatccc tgcccatcaa gttctctttt cctatggtta
     8281 ctgtggcaag cactgtggcc aacttcagac ggttttcatg aagtgtccac aggaagatgg
     8341 gggatgtagt aaaatcccag ctcagctcag aatcaaggtg tggttcggac tgactgcgga
     8401 tgtcaaatct tttaaccagt ttgcagaggg caaactctca gtgtttgcag aaacgtatga
     8461 gaaccagact aaactggctc tggtggggaa ctggggtacg acgggtctga cttaccccaa
     8521 gttcagtgat gtgaccggca gagtcaaact gccaaaggag agcttcaaac cctcttcagg
     8581 ctggagctgg gcaggagaat ggttcatcag cccagagaaa acgatgctgt atgatgctga
     8641 tgcaggtcat ataagctttt tggaggaggt gtttgaaaat cagttgcggt tgccaggagg
     8701 acagtggatt ggcatgccgg aggggtacac agatgtgaat ggagaaaagg ctgttcctaa
     8761 agatgagatc gagtgtcccc ctggctgggt gtgggaggat attgagtgga gcgaggatct
     8821 gaagcgtgca gtggatgatc aagggtggga atatggaata accctccccc cggaccgccg
     8881 accaaagtcc tgggttccat ctgagaagat gtaccatacc aaccgtcgga gacgctggat
     8941 cagactccgc cgtagagaca tgcagaaaat ggaggcgctc agaaaacagc ggccagacga
     9001 gtccgagcgt gagggctggg aatatgcctc tttatttggc tggaagtttc acctcaagca
     9061 gaagaaaacc gacttgttcc gtcgccggcg ctggagacgt cgaatggagc cattagaaaa
     9121 gacagggccg gcggccatct ttgccttgga gtgttcgctg agcagcttta ctgatgaaaa
     9181 gaatgacgat aagtcagtca ctacaacgtt tggtgtcagc cggccgacta tctcctgctt
     9241 ctttgatagt gggaccctgt atcatttgcg ctgttacttg tatcaggcca gagacctgct
     9301 ggctatggac aaggacagtt tctcggatgc ctatgccgtt gtatcgttcc tgcatcagag
     9361 tcagaagacg gtgactgttc gaaacacact caaccccacc tgggaccaga ccctcatttt
     9421 ctacgaagtg gaaatttttg gagaccatct tgtgactgag aggagtcctc caaacattgt
     9481 ggtggagctg tacgatcagg acacctatgg agctgatgag ttcatgggcc gctgtgtttg
     9541 ccaaccatca atggcatctt cccctcgcct cgcctggtat cccatccaga aagcagggaa
     9601 ttctgcagga gatctgcttg ctgcttttga actgatccgc agagataaac ctgcagttca
     9661 tcacatcccg ggtcaagagg gagatattat accaccctca catatcatgg atgagcttgt
     9721 gcttccaggg ttcctctctg agaatcaaca gcaatggcca gaggactctg atttgcccta
     9781 tccaccacct cagagggagc caaacgtcta catggtgcca caggggataa aacctgtcct
     9841 acagcgcact gctattgaga tactggcatg gggtttgcgt aacctgaaga gtttccagct
     9901 ggccagtgtc acgtctccca gtcttcaggt ggaatgtggc ggatctgtgg ttcagtcgtg
     9961 cgtcattcga aatattaaga agaaccctaa ttttgacgtc aacatcctgt ccattgatgt
    10021 ccgattgcct aaggaagagc tctacatgcc cccattggtg atcaaggtta tagacaaccg
    10081 gcagtttggc aggaagcctg tggtgggcca gtgttcagtc cactcgctca atgagttcta
    10141 cttccagccg gaaagtctgg agagagactc aacagaagag gaatttgaag ttattgctca
    10201 cacacctaga gatgatgttc tggttgatat tgatgacaag gagcctctaa tccctggaca
    10261 gcaggatgaa tttatggact ggtggagcaa gttttatgcc tccatgggtg aaaggaccaa
    10321 gtgtgggagt tatctggaga aagggttcga tatgctgcag gtgtatgaca gagaactgga
    10381 gaagactgaa ggctttgagc aatttgctga tttctgtcaa acgtttaaac tttaccgtgg
    10441 aagaacccag aatgacagcg aagatccttc cgttgttgga gagttcaagg gttcgtttaa
    10501 gatctatcca ttgcctgatg atccccctat gcctgtaccc acacgtcagt ttcgcaaact
    10561 ccccccgaac ggtatagagg agtgcttggt acgcatctac atcatccagg cttttgggtt
    10621 acagcccaaa gatgccaatg gcaagtgtga cccctacgtt aagatcacgc ttggcaagaa
    10681 atcagtcgat gaccacgaca attacatacc ctgtactttg gagcccgtct ttggaaagat
    10741 gtttgagctg acctgctgtc tgcctcttga gaaagacctg aagattacac tctatgatta
    10801 cgacatggtg actaaagatg agaaaatcgg agagaccatc attgatttgg agaaccgctt
    10861 cttgtctcgt catggtgctc gctgcggcct tccgcagtcc tactgtctgt caggaattaa
    10921 ccagtggcga gatcaactta aaccatctca gctacttcat cgtctgtgtg agaggcgaaa
    10981 ctacaaactc cccgtctaca agcaggatcg aatttacttc agaggacatg aatacactgt
    11041 cgcagacctt gatgaaggga aacctcttaa ccctcacttg ggaccagtga tggagcgtct
    11101 ggctctgttg gtgttgagga ggcatggttt ggtgcctgaa catgtcgaga cgcgacccct
    11161 ctacagcccc cttcagccag atatagaaca ggggcgacta caaatgtggg tggacctgtt
    11221 ccctaagtct ttaggccctc caggtcctcc attcaacatc accccacgca aagctaaaaa
    11281 gttcttcctg cgctgtatta tatggaacac aagagatgta atcttggatg atgtcagcgt
    11341 taccggggag aagatgagtg acatttatgt gaaaggctgg atgcacggcc atgaggagaa
    11401 caagcagaaa acagacgtgc actaccgatc gctgggtgga gagggtaact ttaactggcg
    11461 cttcctcttc cccttccact atttacctgc ggaacaactg tgcaccattg ataaaaagga
    11521 gcacttttgg agtctggata aagttgaaac gaagcttcct cctaaactta ctatccagat
    11581 ttgggacaat gacaagttct cctttgatga tttcctcggc tctttggaga tggatctgaa
    11641 ccacatgctg agtcctgcga agtctccaga gaagtgtggt cttgagatgc tcactcagtc
    11701 ccaggacaaa ctggtctctt tgcttgagca gaaaacggtg aaaggctggt ggccgtgtgt
    11761 ctgtgacaaa gaagacaaga agatacttgc tggaaaggtg gagatgtctc tggaaatagt
    11821 ctgtgaacag gagcaggatg agaggcctgc aggactcggc cgagatgagc ccaacatgaa
    11881 tccccatcta gaggagcctc agcgtcccga gacatcattc ttatggttct cgtccccata
    11941 taagacgttt aggttcattg tgtggaggag gtttaagtgg cttattctgg gcctctttat
    12001 actccttttc attattctct tccttggagt atttctctac gccttcccta actatgctgc
    12061 tatgaagatg gttggaccct tcagtggact aggaaaggga acacagtgac aactttatta
    12121 tacatagttg ataattcact ggccgtcgtt ttacggtacc atcgatgatg atccagacat
    12181 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
    12241 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
    12301 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
    12361 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatca
    12421 gatctgcgaa gatacggcca cgggtgctct tgatcctgtg gctgattttg gactgtgctg
    12481 ctcgcagctg ctgatgaatc acatacttcc tccattttct tccactgatt gactgttata
    12541 atttccctaa tttccaggtc aaggtgctgt gcattgtggt aatagatgtg acatgacgtc
    12601 acttccaaag gaccaatgaa catgtctgac caatttcata taatgtgaaa acgattttca
    12661 taggcagaat aaataacatt taaattaaac tgggcatcag cgcaattcaa ttggtttggt
    12721 aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa
    12781 taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta
    12841 aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag
    12901 tacagtaatc aagtaaaatt actcaagtac tttacacctc tggttcttga ccccctacct
    12961 tcagcaagcc cagcagatcc actagttcta gagcggccgc caccgcggtg gagctccagc
    13021 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
    13081 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
    13141 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    13201 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    13261 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    13321 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    13381 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    13441 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    13501 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    13561 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    13621 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    13681 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    13741 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    13801 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    13861 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    13921 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    13981 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    14041 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    14101 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    14161 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    14221 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    14281 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    14341 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    14401 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    14461 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    14521 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    14581 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    14641 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    14701 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    14761 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    14821 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    14881 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    14941 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    15001 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    15061 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    15121 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    15181 ataggggttc cgcgcacatt tccccgaaaa gtg
//
