LOCUS       sequence-20644-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(27..151)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(159..175)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(159..175)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(181..444)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(264..287)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
       61 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      121 tttctcgttc agcttttttg tacaaacttg tgatggggga tccactagtt ctagaatccg
      181 tcgaaactaa gttctggtgt tttaaaacta aaaaaaagac taactataaa agtagaattt
      241 aagaagttta agaaatagat ttacagaatt acaatcaata cctaccgtct ttatatactt
      301 attagtcaag taggggaata atttcaggga actggtttca accttttttt tcagcttttt
      361 ccaaatcaga gagagcagaa ggtaatagaa ggtgtaagaa aatgagatag atacatgcgt
      421 gggtcaattg ccttgtgtca tcatttactc caggcaggtt gcatcactcc attgaggttg
      481 tgcccgtttt ttgcctgttt gtgcccctgt tctctgtagt tgcgctaaga gaatggacct
      541 atgaactgat ggttggtgaa gaaaacaata ttttggtgct gggattcttt ttttttctgg
      601 atgccagctt aaaaagcggg ctccattata tttagtggat gccaggaata aactgttcac
      661 ccagacacct acgatgttat atattctgtg taacc
//
